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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2012 / Cilt: 59 - Sayı: 4

Türkiye’de hayvanlardan izole edilen Escherichia coli suşlarında plasmid ilişkili kinolon direnci

Plasmid-mediated quinolone resistance in escherichia coli strains isolated from animals in Turkey

Tuba İÇA
Alper ÇİFTCİ
K. Serdar DİKER
H. Kaan MÜŞTAK
Pages
255–258
Publication Date
2022-10-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This is the sole report from Turkey that evaluated the plasmidmediated quinolone resistance in animal isolates of E. coli strains.

📌 3 Key Takeaways

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Objective & Hypothesis:Chromosomal mutations and resistance genes transferred by plasmids are the main factors of quinolone resistance particularly in Escherichia coli strains isolated from both animals and humans.
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Key Findings & Data:In this study a total of 259 E. coli strains were examined for the resistance to nalidixic acid and ciprofloxacin by agar dilution method and for the presence of qnrA, qnrB and qnrS genes that are known as plasmid-mediated quinolone resistance determinants by PCR.
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Conclusion & Impact:This is the sole report from Turkey that evaluated the plasmidmediated quinolone resistance in animal isolates of E. coli strains.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Chromosomal mutations and resistance genes transferred by plasmids are the main factors of quinolone resistance particularly in Escherichia coli strains isolated from both animals and humans. In this study a total of 259 E. coli strains were examined for the resistance to nalidixic acid and ciprofloxacin by agar dilution method and for the presence of qnrA, qnrB and qnrS genes that are known as plasmid-mediated quinolone resistance determinants by PCR. According to antimicrobial susceptibility tests 47 (50.0%) of 94 chicken, 3 (4.5%) of 66 sheep, 5 (9.6%) of 52 cattle and 3 (6.4%) of 47 dog E. coli strains were found to be resistant to ciprofloxacin. Among all plasmid-mediated quinolone resistance determinants that were investigated in this study, only qnrA gene was found in 5 (5.3%) of 94 E. coli chicken isolates. This is the sole report from Turkey that evaluated the plasmidmediated quinolone resistance in animal isolates of E. coli strains.

Özet

Hayvanlarda ve insanlarda çok çeşitli infeksiyonlara neden olan Escherichia coli’lerde kinolon direnç gelişimine neden olan başlıca faktörler kromozomal mutasyonlar ve plazmidler ile aktarılan direnç genleridir. Bu çalışmada toplam 259 E. coli suşu, nalidiksik asit ve siprofloksasin direnci, agar dilusyon yöntemi kullanılarak ve plazmid ilişkili kinolon direnç belirleyicilerinden olan qnrA, qnrB ve qnrS genlerinin varlığı PCR yöntemi kullanılarak araştırıldı. Antibiyotik duyarlılık test sonuçlarına göre 94 tavuk E. coli suşundan 47 (%50)’si, 66 koyun E. coli suşundan 3 (%4.5)’ü, 52 sığır E. coli suşundan 5 (%9.6)’i, 47 köpek E. coli suşundan 3 (%6.4)’ü siprofloksasin’e dirençli bulundu. Bu çalışmada araştırılan bütün plazmid ilişkili kinolon direnç belirleyicileri içerisinden, izole edilen 94 E. coli tavuk suşu arasında qnrA geni, sadece 5 (%5.3) tavuk izolatında bulundu. Bu çalışma, Türkiye’de E. coli hayvan izolatlarında plazmid ilişkili kinolon direncinin değerlendirildiği tek rapordur.

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Cite This Article

İÇA, T., ÇİFTCİ, A., DİKER, K. S., MÜŞTAK, H. K. (2022). Türkiye’de hayvanlardan izole edilen Escherichia coli suşlarında plasmid ilişkili kinolon direnci. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 59 - Sayı: 4, 255–258. https://ajindex.com/articles/444897

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