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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2012 / Cilt: 59 - Sayı: 2

Bordetella bronchiseptica fimbriya proteinlerinin farelerde immunojenite ve koruyuculuk etkisinin araştırılması

Investigation of immunogenicity and protective efficiency of Bordetella bronchiseptica fimbriae in mice

Özlem BÜYÜKTANIR
Erkan ÖZCENGİZ
Mehmet AKAN
Pages
95–101
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, fimbrial antigens were determined to have important protective efficacy and anti-fimbriae antibody levels were found correlated with that protective efficacy.

📌 3 Key Takeaways

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Objective & Hypothesis:Summary: Fimbrial proteins are important for adhesion and colonization of Bordetella bronchiseptica in the upper respiratory tract.
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Key Findings & Data:In microagglutination test, whole cell F415 was used as the antigen and the agglutination of sera from mice immunized with the purified fimbrial proteins was observed at titers of 1:128 for strains 219 and 4617 and the isolate CA; 1:256 for strain F415 and the isolate UK7; 1:512 for the isolate UK6 and 1:4096 for strain 3036.
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Conclusion & Impact:In conclusion, fimbrial antigens were determined to have important protective efficacy and anti-fimbriae antibody levels were found correlated with that protective efficacy.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Summary: Fimbrial proteins are important for adhesion and colonization of Bordetella bronchiseptica in the upper respiratory tract. In this study, determination of immunogenicity of the fimbrial proteins isolated from different strains of B. bronchiseptica and their protective efficacy against letal challenge was aimed. For this purpose, fimbrial proteins of seven standard strains and clinical isolates of B. bronchiseptica were partially purified by gel filtration chromatography and analysed by SDSPAGE. In microagglutination test, whole cell F415 was used as the antigen and the agglutination of sera from mice immunized with the purified fimbrial proteins was observed at titers of 1:128 for strains 219 and 4617 and the isolate CA; 1:256 for strain F415 and the isolate UK7; 1:512 for the isolate UK6 and 1:4096 for strain 3036. In addition, by ELISA against F415 fimbrial antigen, the antifimbriae antibody levels of the immune mouse sera were observed at serum dilutions of 1:6400 for UK7; 1:3200 for UK6, F415 and 3036; 1:1600 for CA; 1:800 for 219 and 1:400 for 4617. The protective values of the fimbrial proteins against letal challenge with strain F415 in mice immunized with the fimbrial proteins (2x25 μg/dose) were; 100% for F415 and 3036, 84% for UK6 and 50% for UK7, but no protection was observed with the other strains and isolates. In conclusion, fimbrial antigens were determined to have important protective efficacy and anti-fimbriae antibody levels were found correlated with that protective efficacy.

Özet

Özet: Bordetella bronchiseptica’nın üst solunum yollarında adhezyonu ve kolonize olmasında fimbriya proteinleri önemlidir. Bu çalışmada, B. bronchiseptica’nın farklı suşlarından elde edilen fimbriyal proteinlerin immunojenitesi ve canlı bakteri uygulamasına karşı koruyucu etkinliğinin belirlenmesi amaçlandı. B. bronchiseptica’ya ait toplam 7 standart suş ve klinik izolata ait fimbriya proteinleri, jel filtrasyon kromatografisi yoluyla kısmi olarak saflaştırıldı ve SDS-PAGE ile analiz edildi. Saflaştırılan fimbriya proteinleri ile bağışıklanan farelerin serumlarıyla F415 suşuna karşı yapılan mikroaglutinasyon testinde; 219 ve 4617 suşları ile CA izolatı için 1:128, F415 suşu ve UK7 izolatı için 1:256, UK6 izolatı için 1:512 ve 3036 suşu için 1:4096 titrede aglutinasyon gözlendi. Ayrıca, bağışık fare serumlarında, F415 fimbriya antijeni kullanılarak yapılan ELISA’da, UK7 için 1:6400; UK6, F415 ve 3036 için 1:3200; CA için 1:1600; 219 için 1:400 ve 4617 için 1:400 titrede antikor düzeyi belirlendi. Fimbriya proteinleri ile bağışıklanan farelerde (2x25 μg/doz), F415 suşu ile yapılan canlı bakteri uygulamasında ise fimbriya proteinlerinin koruyucu değerleri; F415 ve 3036 suşları için %100, UK6 için %84 ve UK7 için %50 olarak saptandı, diğer suş ve izolatlar için herhangi bir koruyucu değer gözlenmedi. Sonuç olarak, fimbriya antijenlerinin önemli koruyucu etkinliğe sahip olduğu ve anti-fimbriya antikor düzeyi ile bu koruyucu etkinlik arasında uyumlu bir ilişki olduğu belirlendi.

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Cite This Article

BÜYÜKTANIR, Ö., ÖZCENGİZ, E., AKAN, M. (2022). Bordetella bronchiseptica fimbriya proteinlerinin farelerde immunojenite ve koruyuculuk etkisinin araştırılması. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 59 - Sayı: 2, 95–101. https://ajindex.com/articles/444892

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