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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2012 / Cilt: 59 - Sayı: 3

Urine 8-Hydroxy-2’-Deoxyguanosine (8-OHdG) Levels of Dogs in Pyoderma

Piyodermalı köpeklerde idrarda 8-hidroksi-2’-deoksiguanozin (8-OHdG) düzeyleri

Nazlı ERCAN
Ulvi Reha FİDANCI
Pages
163–168
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion in our opinion untreated pyoderma cases could result in DNA damage.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, it is aimed to investigate correlation between antioxidative metabolism and the significant skin disease pyoderma in dogs and to demonstrate the extent of oxidative damage with 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels.
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Key Findings & Data:Healthy (n=13), females diagnosed as pyoderma (untreated) (n=16), heathy (n=14) males and males diagnosed as pyoderma (untreated) (n=12) consisted the four study groups.
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Conclusion & Impact:In conclusion in our opinion untreated pyoderma cases could result in DNA damage.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, it is aimed to investigate correlation between antioxidative metabolism and the significant skin disease pyoderma in dogs and to demonstrate the extent of oxidative damage with 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels. Healthy (n=13), females diagnosed as pyoderma (untreated) (n=16), heathy (n=14) males and males diagnosed as pyoderma (untreated) (n=12) consisted the four study groups. In this aspect two control and two diseased groups were formed according to gender. Blood and urine samples were obtained from all the groups and bacterial swabs were collected from the diseased groups as well. Plasma malondialdehyde (MDA) besides urine 8-hydroxy-2'-deoxyguanosine levels measured with LC/MS/MS. MDA and 8- OHdG levels were increased in diseased groups. Differences among groups were found to be significant (p ≤ 0.05). In conclusion in our opinion untreated pyoderma cases could result in DNA damage.

Özet

Bu çalışmada, köpeklerin önemli bir deri hastalığı olan piyoderma ile antioksidatif metabolizma arasındaki ilişkinin incelenmesi ve oksidatif hasarın boyutunun 8-hidroksi-2’-deoksiguanozin (8-OHdG) üzerinden gösterilmesi amaçlanmıştır. Çalışmada sağlıklı dişi köpekler (n=13) ile piyoderma tanısı konmuş (tedavi almamış) dişi köpekler (n=16) ve sağlıklı erkek köpekler (n=14) ile piyoderma tanısı konmuş (tedavi almamış) erkek köpekler (n=12) olmak üzere cinsiyete göre iki kontrol ve iki hasta grubu oluşturulmuştur. Hasta gruptan kan, idrar ve svap, sağlıklı gruptan kan ve idrar örnekleri alınmıştır. Plazmada malondialdehit (MDA) ve idrarda LC/MS/MS ile 8-hidroksi-2’-deoksiguanozin düzeyleri ölçülmüştür. Piyoderma tanısı konmuş erkek ve dişi köpeklerde MDA ve 8-OHdG düzeylerinin yükseldiği saptanmıştır. Bu artış, istatistik olarak da anlamlı bulunmuştur (p ≤ 0.05). Sonuç olarak piyodermaların, tedavi edilmediği takdirde DNA hasarına yol açabileceği kanısına varılmıştır.

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Cite This Article

ERCAN, N., FİDANCI, U. R. (2022). Urine 8-Hydroxy-2’-Deoxyguanosine (8-OHdG) Levels of Dogs in Pyoderma. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 59 - Sayı: 3, 163–168. https://ajindex.com/articles/444869

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