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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2020 / Cilt: 67 - Sayı: 2

Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour

Venereal tümörlü Kangal köpeklerinde 8-hidroksi-2’deoksiguanozin, malondialdehit düzeyleri ile antioksidan enzim aktivitelerinin belirlenmesi

Murat YÜKSEL
Nazlı ERCAN
Mustafa KOÇKAYA
Pages
121–125
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Serum 8-OHdG and MDA levels of dogs with TVT were found to be significantly increased compared to the control group (P

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Canine transmissible venereal tumour (TVT) is a contagious cancer and sexually transmitted one dog to another by allogenic transfer of living cancer cells.
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Key Findings & Data:The aim of this study is to examine the relationship between antioxidative metabolism and venereal tumour at Kangal Dogs and to show the size of oxidative damage it causes through 8-hydroxy-2’deoxyguanosine (8-OHdG).
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Conclusion & Impact:Serum 8-OHdG and MDA levels of dogs with TVT were found to be significantly increased compared to the control group (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Canine transmissible venereal tumour (TVT) is a contagious cancer and sexually transmitted one dog to another by allogenic transfer of living cancer cells. The aim of this study is to examine the relationship between antioxidative metabolism and venereal tumour at Kangal Dogs and to show the size of oxidative damage it causes through 8-hydroxy-2’deoxyguanosine (8-OHdG). The materials of this study included the blood sera of 15 Kangal Dogs with TVT, and as a control group of 15 healthy Kangal Dogs. The sera levels of 8-OHdG were determined by competitive enzyme linked immunosorbent assay (ELISA) method. The level of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) which are antioxidative enzymes were investigated by spectrophotometric methods as described. Serum 8-OHdG and MDA levels of dogs with TVT were found to be significantly increased compared to the control group (P

Özet

Transmissible (bulaşıcı) venereal tümör (TVT), canlı kanser hücrelerinin allojenik transferiyle bir köpekten diğer bir köpeğe çiftleşme ile bulaşan bir kanser türüdür. Bu çalışmada, Kangal köpeklerinde önemli bir bulaşıcı kanser türü olan venereal tümör ile antioksidatif metabolizmanın arasındaki ilişkinin incelenmesi ve oksidatif hasarın boyutunun 8 hidroksi-2’deoksiguanozin (8-OHdG) düzeyleri üzerinden gösterilmesi amaçlandı. Bu amaçla, 15 adet TVT’li, kontrol grubu olarak 15 adet sağlıklı Kangal köpeklerinden alınan toplam 30 adet kan serumu çalışmanın materyalini oluşturdu. Bu hayvanlara ait kan serumlarında 8-OHdG kompetitif enzim bağımlı immunosorbent analiz (ELISA) yöntemi ile belirlendi. Malondialdehit (MDA), antioksidan enzimler olan süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx) ve katalaz (CAT) seviyeleri ise spektrofotometrik yöntemlerle analiz edildi. TVT teşhisli köpeklerin serum 8-OHdG ve MDA düzeyleri kontrol grubundaki köpeklere göre istatiksel olarak anlamlı derecede artış gösterdiği tespit edildi (P

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Cite This Article

YÜKSEL, M., ERCAN, N., KOÇKAYA, M. (2022). Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 67 - Sayı: 2, 121–125. https://ajindex.com/articles/438618

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References (15)

  1. Aebi H (1984): Catalase in vitro. Methods Enzymol, 105, 121-6
  2. Aydın İ, Bülbül A, Avcı GE, et al (2009): Serum oxidative status and adenosine deaminase activity in dogs with transmissible venereal tumour. Bull Vet Inst Pulawy, 53, 771-774
  3. Baştan A, Baki Acar D, Cengiz M (2008): Uterine and ovarian metastasis of transmissible venereal tumour in a bitch. Turk J Vet Anim Sci, 32, 65-66
  4. Dinçer Y, Akçay T, Kural AR, et al (2011): Evaluation of 8-hydroxy-2'-deoxyguanosine concentration and antioxidant enzyme activities in bladder cancer patients. Turkiye Klinikleri J Med Sci, 31, 553-558
  5. Durak I, Yurtaslanı Z, Canbolat O, et al (1993): A methodological approach to superoxide dismutase (SOD) activity assay based on inhibition of nitroblue tetrazolium (NBT) reduction. Clin Chim Acta, 214,103-104
  6. Ercan N, Fidancı UR (2012): Piyodermalı köpeklerde 8- hidroksi-2’-deoksiguanozin (8-OHdG) düzeyleri. Ankara Üniv Vet Fak Derg, 59, 163-168
  7. Fraga C, Shigenaga M, Park J, et al (1990): Oxidative damage to DNA during aging: 8-Hydroxy-2’- deoxyguanosine in rat organ DNA urine. Proc Natl Acad Sci, 87, 4533-4537
  8. Gencer M, Ceyla E, Aksoy A, et al (2005): Oksidatif stres benign ve malign akciğer hastalıklarının ayırıcı tanısında belirteç olabilir mi? Akciğer Arşivi, 6, 89-92
  9. Haliwell B (2007): Oxidative stress and cancer: have we moved forward? Biochem J, 401, 1-11
  10. Hattori Y, Nishigori C, Tanaka T, et al (1996): 8- hydroxy-2’-deoxyguanosine is increased in epidermal cells of hairless mice after chronic ultraviolet B exposure. J Invest Dermatol, 107, 733-737
  11. Janero DR (1990): Malondialdehyde and thiobarbituric acid-reactivy as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med, 9, 515-540
  12. Jayasri K, Padmaja K, Saibaba M (2016): Altered oxidative stress and carbohydrate metabolism in canine mammary tumors. Veterinary world, 9, 1489
  13. Kumaraguruparan R, Balachandran C, Manohar BM, et al (2005): Altered oxidant-antioxidant profile in canine mammary tumours, Vet Res Commun, 29, 287-96
  14. Lin H, Jenner A, Ong C, et al (2004): A high-throughput and sensitive methodology for the quantification of urinary 8-hydroxy-2’-deoxyguanosine: measurement with gas chromatography-mass spectrometry after single solidphase extraction. Biochem J, 380, 541-548
  15. Macotpet A, Suksawat F, Sukon P, et al (2013): Oxidative stress in cancer-bearing dogs assessed by measuring serum malondialdehyde. BMC Vet Res, 9,