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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2017 / Cilt: 64 - Sayı: 4

Isolation of Listeria monocytogenes in lamb meat and determination of the antibiotic resistance

Kuzu etlerinden Listeria monocytogenes izolasyonu ve antibiyotik dirençliliklerinin belirlenmesi

Nurcay KOCAMAN
Belgin SARIMEHMETOĞLU
Pages
273–279
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Among the five isolates, four (6.9%) displayed multiple resistance, only one isolate was resistant to tetracycline

📌 3 Key Takeaways

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Objective & Hypothesis:In this study the occurrence of Listeria monocytogenes and other Listeria species were investigated from a total of 120 retail raw lamb meat samples and L. monocytogenes isolates were verified by using PCR based on hylA gene which is responsible for virulence.
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Key Findings & Data:L. monocytogenes was isolated from 19 (15.8%) of the samples examined.
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Conclusion & Impact:Among the five isolates, four (6.9%) displayed multiple resistance, only one isolate was resistant to tetracycline
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study the occurrence of Listeria monocytogenes and other Listeria species were investigated from a total of 120 retail raw lamb meat samples and L. monocytogenes isolates were verified by using PCR based on hylA gene which is responsible for virulence. All L. monocytogenes isolates were analyzed by antimicrobial susceptibility tests. L. monocytogenes was isolated from 19 (15.8%) of the samples examined. The other species isolated were L. innocua, L. weshimeri and L. grayi 26 (21.6%), nine (7.5%) 2 (1.6%) strains respectively. According to the PCR analysis, hlyA gene was detected from the 58 of the isolates which were isolated from L. monocytogenes positive 19 lamb meat samples. The antibiotic resistance profiles of the 58 isolates to nine antibiotics were detected by disc diffusion method. Five (8.6%) isolates were found to be resistant to antibiotics. Among the five isolates, four (6.9%) displayed multiple resistance, only one isolate was resistant to tetracycline

Özet

Bu çalışmada, satışa sunulan toplam 120 çiğ kuzu eti örneklerinde Listeria monocytogenes ve diğer Listeria türlerinin varlığı araştırılmış ve bulunan L. monocytogenes izolatları virülensten sorumlu hylA geni esas alınarak PZR ile doğrulanmıştır. Bütün L. monocytogenes izolatları antibiyotik duyarlılık testi ile analiz edilmiştir. Test edilen örneklerin 19'undan (15.8%) L. monocytogenes izole edilmiştir. İzole edilen diğer türler sırasıyla 26 adet (21.6%) L. innocua, dokuz adet (7.5%) L. weshimeri ve iki adet (1.6%) L. grayi'dir. PZR analizine göre 19 kuzu eti örneğinden izole edilen L. monocytogenes pozitif izolatların 58'inde hlyA geni belirlenmiştir. 58 izolatın dokuz antibiyotiğe karşı antibiyotik direnç profili disk difüzyon metodu kullanılarak belirlenmiştir. Beş izolatın (8.6%) antibiyotiklere karşı dirençli olduğu bulunmuştur. Bu beş izolat arasında dördü (6.9%) çoklu direnç gösterirken, bir izolat sadece tetrasikline karşı dirençli bulunmuştur

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Cite This Article

KOCAMAN, N., SARIMEHMETOĞLU, B. (2022). Isolation of Listeria monocytogenes in lamb meat and determination of the antibiotic resistance. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 64 - Sayı: 4, 273–279. https://ajindex.com/articles/444649

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