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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2017 / Cilt: 64 - Sayı: 1

Düşük doz kapsaisinin rat duodenumunda substans P ekspresyonu üzerine etkileri

The effects of low dose capsaicin on substance P expression in rat duodenum

Tuncay İLHAN
Cansel Güzin AKKOÇ ÖZGÜDEN
Berrin ZIK
Sabire PEKER
Pages
31–36
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Low dose CAP did not alter the depth of crypt in the duodenum but the height of the villus was higher than the other group (p

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of the present study was to investigate effects of low dose capsaicin (CAP) on substance P (SP) expression and histomorphometric properties of duodenum.
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Key Findings & Data:In this study, 21-day-old rats were divided into three groups as control, CAP-treated and vehicle.
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Conclusion & Impact:Low dose CAP did not alter the depth of crypt in the duodenum but the height of the villus was higher than the other group (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of the present study was to investigate effects of low dose capsaicin (CAP) on substance P (SP) expression and histomorphometric properties of duodenum. In this study, 21-day-old rats were divided into three groups as control, CAP-treated and vehicle. The control group remained without any treatment. 0.5 mg/kg CAP prepared in a solvent and injected subcutaneously to CAP-treated group and vehicle group was injected with only solvent for 20 days. All rats were weighed daily. At the end of the experiment, tissue samples were collected and Crossman's triple staining was used for histomorphometric examinations and labelled streptavidin-biotin immunohistochemical technique was used for SP expression. The body weight gain was lower in the CAP treated group but no significant differences between the groups. Low dose CAP did not alter the depth of crypt in the duodenum but the height of the villus was higher than the other group (p

Özet

Sunulan çalışmanın amacı, düşük doz kapsaisinin (CAP) duodenumun histomorfometrik yapısı ve substans P (SP) ekspresyonu üzerine etkisini incelemektir. Çalışmada kullanılan ratlar (21 günlük), kontrol grubu, CAP enjekte edilen grup ve çözücü solüsyon enjekte edilen olmak üzere üç gruba ayrıldı. Kontrol grubuna enjeksiyon yapılmadı. 20 gün boyunca CAP grubuna çözücü solüsyon içerisinde hazırlanan 0.5 mg/kg kapsaisin, taşıyıcı grubuna ise sadece çözücü solüsyon subkutan enjekte edildi. Ratlar her gün tartıldı. Deney sonrası dokular alınarak, doku kesitlerine histomorfometrik incemeler için Crossman'ın üçlü boyaması, SP ekspresyonunu belirlemek için ise işaretlenmiş streptavidin-biotin immunohistokimyasal tekniği uygulandı. CAP uygulanan grupta canlı ağırlık kazancı kontrol gruplarına göre düşüktü, fakat gruplar arasında istatiksel farklılık gözlenmedi. Düşük doz CAP duodenumun kript derinliğini etkilemezken, villus uzunluğu CAP uygulanan grupta diğer gruplara göre daha yüksek belirlendi (p

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Cite This Article

İLHAN, T., ÖZGÜDEN, C. G. A., ZIK, B., PEKER, S. (2022). Düşük doz kapsaisinin rat duodenumunda substans P ekspresyonu üzerine etkileri. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 64 - Sayı: 1, 31–36. https://ajindex.com/articles/444645

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