SavedAdd your journal

Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2018 / Cilt: 65 - Sayı: 2

Akkaraman koç spermasının 7-dehidrokolesterol ile dondurulması ve değerlendirilmesi

Cryopreservation and evaluation of Akkaraman ram semen with 7-dehydrocholesterol

Muhammed Enes İNANÇ
Ongun UYSAL
Ayhan ATA
Pages
187–192
Publication Date
2022-10-12
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, it can be said that adding 7-DCLC to Akkaraman ram semen improved cell membrane cryosurvival.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The experiment was conducted to determine the in vitro results of 7-dehydrocholesterol loaded cyclodextrin compound (7-DCLC) addition to tris extenders for freezing ram semen.
📊
Key Findings & Data:Semen samples were extended with tris containing 0 mg (control), 1.5 mg, 2.5 mg and 3.5 mg/120x106 7-DCLC and they were equilibrated to 4°C and frozen in mini straws then stored in liquid nitrogen.
💡
Conclusion & Impact:In conclusion, it can be said that adding 7-DCLC to Akkaraman ram semen improved cell membrane cryosurvival.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The experiment was conducted to determine the in vitro results of 7-dehydrocholesterol loaded cyclodextrin compound (7-DCLC) addition to tris extenders for freezing ram semen. Three Akkaraman rams, local species in Turkey, were used. Semen samples were extended with tris containing 0 mg (control), 1.5 mg, 2.5 mg and 3.5 mg/120x106 7-DCLC and they were equilibrated to 4°C and frozen in mini straws then stored in liquid nitrogen. Straws were thawed at 37°C for 30 s in water bath for spermatological examination. Statistically significant differences were observed between 7-DCLC and control groups in terms of acrosome integrity, CASA motility, HOS test and viability after thawing (P 0.05). In conclusion, it can be said that adding 7-DCLC to Akkaraman ram semen improved cell membrane cryosurvival. Besides, 7-DCLC can be used in tris extender instead of cholesterol loaded cyclodextrin (CLC) compounds for sperm cryopreservation

Özet

Bu çalışma, tris sulandırıcısına 7-dehidrokolesterol ile doyurulmuş siklodekstrin bileşimi (7-DCLC) eklenerek dondurmaçözdürme sonrası in vitro sonuçlarını değerlendirmek üzere yapıldı. Çalışmada yerli bir ırk olarak üç adet Akkaraman koç kullanıldı. Sperma örnekleri 0 mg (kontrol), 1.5, 2.5 ve 3.5 mg/120x106 7-DCLC içeren tris sulandırıcısı ile sulandırıldı, 4 C?'de alışıma bırakıldı ve mini payetlerde dondurularak sıvı azotta saklandı. Daha sonra payetler spermatolojik analizler için 37 C?'de 30 saniyede su banyosunda çözdürüldü. Çözüm sonu, 7-DCLC grupları ve kontrol grubu arasında CASA motilitesi, canlılık, akrozomal bütünlük ve HOS test yönünden istatiksel olarak farklılık önemli bulundu (P0.05). Çalışma sonucunda, 7-DCLC'nin Akkaraman koç spermasına eklenmesinin hücre membranlarını soğuktan koruduğu söylenebilir. Ayrıca, spermanın dondurulması için tris sulandırıcılarında kolesterol ile doyurulmuş siklodekstrin (CLC) bileşimi yerine 7-DCLC'nin kullanılabileceği tespit edildi

🔖

Cite This Article

İNANÇ, M. E., UYSAL, O., ATA, A. (2022). Akkaraman koç spermasının 7-dehidrokolesterol ile dondurulması ve değerlendirilmesi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 65 - Sayı: 2, 187–192. https://ajindex.com/articles/444583

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (16)

  1. Aisen EG, Medina VH, Venturino A (2002): Cryopreservation and post-thwed fertility of ram semen frozen in different trehalose concentrations. Theriogenology, 57, 1801-1808
  2. Amorim EAM, Graham JK, Spizziri B, et al. (2009): Effect of cholesterol or cholesteryl conjugates on the cryosurvival of bull sperm. Cryobiology, 58, 210-214
  3. Award MM (2011): Effects of sub-optimal glycerol concentration and cholesterol-loaded cyclodextrin in a Tris-based diluent on cryopreserved ram sperm longevity and acrosomal integrity. Small Rumin Res, 100, 164-168
  4. Barrera-Compean MH, Purdy PH, Dzakuma JM et al. (2005): Cholesterol-loaded cyclodextrin improves postthaw goat sperm motility. J Anim Sci, 83 (Suppl. 1), abstract T91
  5. Başpinar N, Çoyan K, Bucak MN, et al. (2011): Effects of dithioerythritol on ram semen after the freeze–thawing process. Cryobiology, 63, 152-156
  6. Bucak MN, Tekin N (2007): Kriyoprotektanlar ve gamet hücrelerinin dondurulmasında kryoprotektif etki. Ankara Üniv Vet Fak Derg, 54, 67-72
  7. Bucak MN, Keskin N, Taşpınar M, et al. (2013): Raffinose and hypotaurine improve the post-thawed Merino ram. Cryobiology, 67, 34-39
  8. Bucak MN, Ataman MB, Başpınar N, et al. (2015): Lycopene and resveratrol improve post-thaw bull sperm parameters: Sperm motility, mitochondrial activity and DNA integrity. Andrologia, 47, 545-552
  9. Comercio EA, Monachesi E, Loza ME, et al. (2013): Hypo-osmotic test in cat spermatozoa. Andrologia, 45, 310- 314
  10. Conell MO, McClure N, Lewis SEM (2002): The effect of cryopreservation on sperm morphology, motility and mitochondrial function. Human Reprod, 17, 704-709
  11. Çoyan K, Başpınar N, Bucak MN, et al. (2011): Effects of cysteine and ergothioneine on post-thawed Merino ram sperm and biochemical parameters. Cryobiology, 63, 1-6
  12. Garner DL, Hafez ESE (1993): Reproduction in Farm Animals, Spermatozoa and seminal plasma. Lea & Febier, Philadelphia
  13. Garner DL, Johnson LA (1995): Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biol Reprod, 53, 276-284
  14. Graaf De SP, Evans G, Gillan L, et al. (2007): The influence of antioxidant, cholesterol and seminal plasma on the in vitro quality of sorted and nonsorted ram spermatozoa. Theriogenology, 67, 217-227
  15. Holt WV (2000): Basic aspects of frozen storage of semen. Anim Reprod Sci, 62, 3-22
  16. Inoue K, Kubota S, Seyama Y (1999): Cholestanol induces apoptosis of cerebellar neuronal cells, biochemical and biophysical. Biochemical an