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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2019 / Cilt: 66 - Sayı: 1

Relationships between Abelson tyrosine kinase (c-abl) and spindle during meiotic division in mouse oocyte in vitro

Fare oositlerinde mayoz bölünme sırasında Abelson tirozin kinaz (c-abl) ile mekik iplikleri arasındaki in vitro iliş

Hakan ÖNER
Duygu MUTLUAY
Pages
89–93
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results demonstrate that c-Abl may play role in asymmetric division of the egg to produce polar body, positioning of the spindle, and may regulate the microtubule cytoskeleton or have some roles in this process.Keywords: c-Abl, meiotic division, oocyte activation, spindle positioning

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The c-Abl gene is a nonreceptor tyrosine kinase that has roles in cell growth, control of the cell cycle, initiation of pronuclear movement and actin cytoskeleton dynamics.
📊
Key Findings & Data:The present study has examined the localization of c-Abl protein during oocyte activation in mouse in vitro.
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Conclusion & Impact:These results demonstrate that c-Abl may play role in asymmetric division of the egg to produce polar body, positioning of the spindle, and may regulate the microtubule cytoskeleton or have some roles in this process.Keywords: c-Abl, meiotic division, oocyte activation, spindle positioning
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The c-Abl gene is a nonreceptor tyrosine kinase that has roles in cell growth, control of the cell cycle, initiation of pronuclear movement and actin cytoskeleton dynamics. The present study has examined the localization of c-Abl protein during oocyte activation in mouse in vitro. Confocal immunofluorescence analysis showed that c-Abl originates at the cortex with a crescent shape before the activation and continues up along both sides of the cortex after activation during meiotic division until pronuclear stage. These results demonstrate that c-Abl may play role in asymmetric division of the egg to produce polar body, positioning of the spindle, and may regulate the microtubule cytoskeleton or have some roles in this process.Keywords: c-Abl, meiotic division, oocyte activation, spindle positioning

Özet

c-Abl geni, hücre büyümesi, hücre döngüsünün kontrolü, pronüklear hareketin başlatılması ve aktin sitoskeleton dinamikleri üzerinde rol oynayan bir nonreseptör tirozin kinazdır. Bu çalışmada, fare de oosit aktivasyonu sırasında c-Abl proteininin lokalizasyonu in vitro olarak incelenmiştir. Konfokal immünfloresans analizi, c-Abl'in aktivasyon öncesinde kortekste yarım ay şeklinde lokalize olurken aktivasyon sonrasında mayoz bölünmeden pronükleus evresine kadar korteksin her iki tarafını sararak yukarı doğru devam ettiği gösterilmiştir. Bu sonuçlar, c-Abl'nin yumurta hücresinin kutup cisimciğinin oluşumu için gerekli olan asimetrik bölünmede, mekik ipliği pozisyonlandırılmasında rol oynayabileceğini ve mikrotübül hücre iskeletini düzenleyebileceğini veya bu süreçte bazı rollere sahip olabileceğini göstermektedir.

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Cite This Article

ÖNER, H., MUTLUAY, D. (2022). Relationships between Abelson tyrosine kinase (c-abl) and spindle during meiotic division in mouse oocyte in vitro. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 66 - Sayı: 1, 89–93. https://ajindex.com/articles/444547

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