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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2021 / Cilt: 68 - Sayı: 3

Molecular identification of Paramphistomidae obtained from ruminants in Van province

Van ilindeki ruminatlardan elde edilen Paramphistomidae’lerin moleküler olarak belirlenmesi

Ayşe KARAKUŞ
Yusuf PADAK
Pages
223–228
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, C. daubneyi, which was previously detected by morphological methods in Turkey, in this study, itwas identified for the first time by using molecular methods.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study was aimed to identify Paramphistomum species from infected ruminants (sheep and cattle) by molecularmethods between March 2018 and September 2018 at Van municipality slaughterhouse.
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Key Findings & Data:In the research, the rDNA ITS-2 gene regionof adult Paramphistomums was amplified by PCR method.
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Conclusion & Impact:In conclusion, C. daubneyi, which was previously detected by morphological methods in Turkey, in this study, itwas identified for the first time by using molecular methods.
🔬Design:Research Article
📍Scope:2018 Verileri
⚙️Variables:Domain Parameters

Abstract

This study was aimed to identify Paramphistomum species from infected ruminants (sheep and cattle) by molecularmethods between March 2018 and September 2018 at Van municipality slaughterhouse. In the research, the rDNA ITS-2 gene regionof adult Paramphistomums was amplified by PCR method. Amplicons 399 bp long were viewed in agarose gel. As a result ofbidirectional sequence analysis made from PCR amplicons, Paramphistomum leydeni and Calicophoron daubneyi species wereidentifyed. Amplicon sequences were compared by BLAST with reference sequences from Genbank. Phylogenetic tree was createdwith the Neighbor-Joining method by using the MEGA 7 program. Genotypes obtained from isolates were compared for exact orclosest similarities. In conclusion, C. daubneyi, which was previously detected by morphological methods in Turkey, in this study, itwas identified for the first time by using molecular methods. Also in this study, P. leydeni was reported for the first time in Turkey.

Özet

Bu çalışmada, Mart 2018-Eylül 2018 tarihleri arasında Van belediyesi mezbahasında enfekte ruminantlardan (koyun ve sığır) elde edilen Paramphistomum türlerinin moleküler yöntem kullanılarak belirlenmesi amaçlanmıştır. Araştırmada, erişkin Paramphistomum’ların rDNA ITS-2 gen bölgesi PCR yöntemi ile çoğaltıldı. Agaroz jelde, 399 bp uzunluğunda amplikonlar görüntülendi. PCR amplikonlarından yapılan çift yönlü sekans analizi sonucunda Paramphistomum leydeni ve Calicophoron daubneyi türleri belirlendi. Amplicon dizileri, BLAST ile Genbank'taki referans dizilerle karşılaştırıldı. MEGA 7 programı kullanılarak Neighbor-Joining yöntemi ile filogenetik ağaç oluşturuldu. İzolatlardan elde edilen genotipler tam yada en yakın benzerlikleri karşılaştırıldı. Sonuç olarak, Türkiye'de daha önce morfolojik yöntemlerle tespit edilen C. daubneyi, bu çalışmada moleküler yöntemler kullanılarak ilk kez tespit edildi. Ayrıca bu çalışmada, Türkiye'de ilk kez P. leydeni bildirildi.

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Cite This Article

KARAKUŞ, A., PADAK, Y. (2022). Molecular identification of Paramphistomidae obtained from ruminants in Van province. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 68 - Sayı: 3, 223–228. https://ajindex.com/articles/444413

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References (16)

  1. Anderson GR, Barker SC (1998): Inference of phylogeny and taxonomy within the didymozoidae (Digenea) from the second internal transcribed spacer ITS2 of ribosomal DNA. Syst Parasitol, 41, 87-94
  2. Celep A, Açıcı M, Çetindağ M, et al (1990): Samsun yöresi sığırlarında helmintolojik araştırmalar. Etlik Vet Mikrobiyol Derg, 6, 117-30
  3. Coşkun ŞZ (1988): The discovery and spread of Paramphistomum species in ruminants.Turk J Vet Anim Sci, 12, 168-179
  4. Goswami LM, Prasad PK, Tandon V, et al (2009): Molecular characterization of Gastrodiscoides hominis (Platyhelminthes: Trematoda: Digenea) inferred from ITS rDNA sequence analysis. Parasitol Res, 104, 1485-1490
  5. Güralp N (1981): The Helminthology. Ankara University Faculty of Veterinary Medicine. Ankara University Press, Ankara, Turkey
  6. Horak IG (1971): Paramphistomiasis of domestic ruminants. Adv Parasitol, 9, 33-72
  7. Ichikawa M, Kondoh D, Bawn S, et al (2013): Morphological and molecular characterization of Explanatum explanatum from cattle and buffaloes in Myanmar. JVMS, 75, 309-314
  8. Itagaki T, Tsumagari N, Tsutsumi K, et al (2003): Discrimination of three amphistome species by PCR-RFLP based on rDNA ITS2 markers. J Vet Med Sci, 65, 931-933
  9. Jones A (1990): Techniques for hand-sectioning thickbodied Platyhelminths. Syst Parasitol, 15, 211-218
  10. Khan UJ (2008): Epidemiology, Economic Importance and Therapy of Paramphistomosis in Cattle and Buffaloes. PhD Thesis Department of zoology, University of Punjap, Lahor, Pakistan
  11. Lotfy WM, Brant SV, Ashmawy KI, et al (2010): A molecular approach for identification of paramphistomes from Africa and Asia. Vet Parasitol, 174, 234-240
  12. Lotfy WM, Brant SV, DeJong RJ, et al (2008): Evolutionary origins, diversification, and biogeography of liver flukes (Digenea, Fasciolidae). Am J Trop Med Hyg, 79, 248-255
  13. Luton K, Walker D, Blair D (1992): Comparisons of ribosomal internal transcribed spacers from two congeneric species of flukes (Piatyhelminthes: Trematoda: Digenea). Mol Biochem Parasit, 56, 323-328
  14. Martínez-Ibeas AM, González-Warleta M, MartínezValladares M, et al (2013): Rumen fluke in Irish sheep: prevalence, risk factors and molecular identification of two paramphistome species. BMC Vet Res, 12, 143
  15. Özdal N, Gül A, İlhan F, et al (2010): Prevalence of Paramphistomum infection in cattle and sheep in Van Province, Turkey. Helminthologia, 47, 20-24
  16. Reinitz DM, Yoshino TP, Cole RA (2007): A Ribeiroia spp. (Class: Tremato