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Journals / Türk Mikrobiyoloji Cemiyeti Dergisi / 2017 / Cilt: 47 - Sayı: 2

Comparative Evaluation of in Vitro Virulence of Mould Isolates from Clinical Cases and from Air Samples and Their Mortality Rates in Galleria mellonella Larvae

Klinik Örneklerden ve Havadan İzole Edilen Küf Mantarlarının İn Vitro Virülans Faktörlerinin ve Galleria mellonella Larva Ölüm Hızlarının Karşılaştırılması

Hatice ERDEM
Ahmet Kamil KARAKUŞ
Ayşe KALKANCI
Zemfira ALİYEVA
Pages
67–77
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Results: We found no difference between in vitro and in vivo virulence features of the moulds isolated from different sources.Conclusion: The projections of this study on human infections should be evaluated

📌 3 Key Takeaways

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Objective & Hypothesis:Comparative Evaluation of in Vitro Virulence of Mould Isolates from Clinical Cases and from Air Samples and Their Mortality Rates in Galleria mellonella LarvaeObjective: Air-borne moulds are the causative agents of oppurtunistic fungal infections.
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Key Findings & Data:Material and Methods: A total number of isolates from 12 clinical samples and 14 air-borne moulds were evaluated as for casein hydrolysis, phospholipase, esterase activities, DNAse production, esculin hydrolysis, catalase activity and aspartyl proteinase production.
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Conclusion & Impact:Results: We found no difference between in vitro and in vivo virulence features of the moulds isolated from different sources.Conclusion: The projections of this study on human infections should be evaluated
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Comparative Evaluation of in Vitro Virulence of Mould Isolates from Clinical Cases and from Air Samples and Their Mortality Rates in Galleria mellonella LarvaeObjective: Air-borne moulds are the causative agents of oppurtunistic fungal infections. However limited number of in vitro and in vivo comparative studies have been performed about virulence features of these moulds. In this study, we compared the virulence features of different moulds isolated from patients and air samples. Material and Methods: A total number of isolates from 12 clinical samples and 14 air-borne moulds were evaluated as for casein hydrolysis, phospholipase, esterase activities, DNAse production, esculin hydrolysis, catalase activity and aspartyl proteinase production. Galleria mellonella larvae were infected with these clinical and air-borne moulds. In vitro virulence factors and in vivo mortalities were compared. Results: We found no difference between in vitro and in vivo virulence features of the moulds isolated from different sources.Conclusion: The projections of this study on human infections should be evaluated

Özet

Amaç: Havada bulunan küfler fırsatçı mantar enfeksiyonlarının etkenidir. Fakat bu küflerin virülans özellikleri konusunda in vitro ve in vivo karşılaştırmalı çalışmalar sınırlıdır. Bu çalışmada, hastalardan ve havadan izole edilen küflerin virülans özellikleri karşılaştırılmıştır.Gereç ve Yöntem: Toplam olarak 12 klinik izolat ve 14 hava izolatı kazein hidrolizi, fosfolipaz, esteraz, DNAz üretimi, eskülin hidrolizi, katalaz aktivitesi ve salgısal aspartil proteinaz üretimi bakımından incelenmiştir. Bu küfler Galleria mellonella larvalarına enjekte edilerek enfeksiyon oluşturulmuştur. In vitro virülans faktörleri ve in vivo mortaliteleri karşılaştırılmıştır. Bulgular: Farklı kaynaklardan elde edilen küflerin in vitro ve in vivo virülans özellikleri arasında bir fark gösterilememiştir. Sonuç: Bu çalışmanın gerçek insan enfeksiyonundaki yansımaları değerlendirilmelidir

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Cite This Article

ERDEM, H., KARAKUŞ, A. K., KALKANCI, A., ALİYEVA, Z. (2022). Comparative Evaluation of in Vitro Virulence of Mould Isolates from Clinical Cases and from Air Samples and Their Mortality Rates in Galleria mellonella Larvae. Türk Mikrobiyoloji Cemiyeti Dergisi, Cilt: 47 - Sayı: 2, 67–77. https://ajindex.com/articles/635866

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References (10)

  1. Paulussen C, Hallsworth JE, Álvarez-Pérez S, et al. Ecology of aspergillosis: insights into the pathogenic potency of Aspergillus fumigatus and some other Aspergillus species. Microb Biotechnol 2017; 10:296- 322.
  2. McCormick A, Loeffler J, Ebel F. Aspergillus fumigatus: contours of an opportunistic human pathogen. Cell Microbiol 2010; 12:1535-43.
  3. Hogan LH, Klein BS, Levitz SM. Virulence factors of medically important fungi. Clin Microbiol Rev 1996; 9:469-88
  4. Kasprzyk I. Aeromycology-main research fields of interest during the last 25 years. Ann Agric Environ Med 2008; 15:1-7
  5. Jabra-Rizk MA, Kong EF, Tsui C, et al. Candida albicans pathogenesis: Fitting within the host-microbe damage response framework. Infect Immun 2016; 84:2724-39. https://doi.org/10.1128/IAI.00469-16 6. de Repentigny L. Animal models in the analysis of Candida host-pathogen interactions. Curr Opin Microbiol 2004; 7:324-9.
  6. Arvanitis M, Glavis-Bloom J, Mylonakis E. Invertebrate models of fungal infection. Biochim Biophys Acta 2013; 1832:1378-83.
  7. Ortiz GE, Noseda DG, Ponce Mora MC, Recupero MN, Blasco M, Albertó E. A comparative study of new Aspergillus strains for proteolytic enzymes production by solid state fermentation. Enzyme Res 2016; 2016:3016149.
  8. Ellepola AN, Samaranayake LP, Khan ZU. Extracellular phospholipase production of oral Candida albicans isolates from smokers, diabetics, asthmatics, denture wearers and healthy individuals following brief exposure to polyene, echinocandin and azole antimycotics. Braz J Microbiol 2016; 47:911-6.
  9. Fatahinia M, Poormohamadi F, Zarei Mahmoudabadi A. Comparative study of esterase and hemolytic activities in clinically important Candida species, isolated from oral cavity of diabetic and nondiabetic individuals. Jundishapur J Microbiol 2015; 8:e20893.
  10. Jeffries CD, Holtman DF, Guse DG. Rapid method for determining the activity of microorganisms on nucleic acids. J Bacteriol 1957; 73:590-1. 12. de Paula Menezes R, de Melo Riceto ÉB, Borges AS, de Brito Röder DV, dos Santos Pedroso R. Evaluation of virulence factors of Candida albicans isolated from HIV-positive individuals using HAART. Arch Oral Biol 2016; 66:61-5. https://doi.org/10.101