SavedAdd your journal

Journals / Anadolu Kardiyoloji Dergisi / 2009 / Cilt: 9 - Sayı: 4

Sıçan torasik aortunda doksorubisinin oluşturduğu fonksiyon bozukluğunun resveratrol uygulaması ile düzelmesi: Nitrik oksit sentaz inhibisyonunun olası rolü var mı?

Reversal of doxorubicin-induced vascular dysfunction by resveratrol in rat thoracic aorta: Is there a possible role of nitric oxide synthase inhibition?

Murat OLUKMAN
Mehtap Gülcihan ÇINAR
Cenk CAN
Onur ORAL
Ayşe EROL
Gülperi ÖKTEM
Pages
260–266
Publication Date
2022-10-10
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results: Doxorubicin (20 mg/kg, i.p), not only decreased the contractile responses to phenylephrine (p

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: The natural antioxidant, resveratrol has been suggested to protect against doxorubicin-induced cardiotoxicity.
📊
Key Findings & Data:We investigated the impact of resveratrol on doxorubicin-induced vascular dysfunction in rat thoracic aorta with regard to NO synthesis in an experimental, prospective, controlled study.Methods: Wistar rats were assigned to 5 groups; doxorubicin (n=9), vehicle (dimethylsulphoxide) (n=8), resveratrol (n=8), doxorubicin+resveratrol (n=10), controls (n=9).
💡
Conclusion & Impact:Results: Doxorubicin (20 mg/kg, i.p), not only decreased the contractile responses to phenylephrine (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The natural antioxidant, resveratrol has been suggested to protect against doxorubicin-induced cardiotoxicity. Although derangements in nitric oxide (NO) synthesis contribute to vascular endothelial dysfunction caused by doxorubicin, the effects of resveratrol on these parameters have not been evaluated yet. We investigated the impact of resveratrol on doxorubicin-induced vascular dysfunction in rat thoracic aorta with regard to NO synthesis in an experimental, prospective, controlled study.Methods: Wistar rats were assigned to 5 groups; doxorubicin (n=9), vehicle (dimethylsulphoxide) (n=8), resveratrol (n=8), doxorubicin+resveratrol (n=10), controls (n=9). Contractile and relaxant responses were evaluated on the isolated thoracic aortas. The expressions of endothelial (eNOS) and inducible (iNOS) isoforms of NO-synthase were also examined histopathologically on the aortas. Statistical analysis was performed by ANOVA for repeated measures for the response curves and one-way ANOVA for the pD2 (-log EC50) and Emax (maximum phenylephrine contraction) values with subsequent Bonferroni test. Results: Doxorubicin (20 mg/kg, i.p), not only decreased the contractile responses to phenylephrine (p

Özet

Amaç: Doğal bir antioksidan olan resveratrolün doksorubisine bağlı kardiyotoksisiteye karşı koruduğu öne sürülmektedir. Nitrik oksit (NO) sentezindeki düzensizliklerin doksorubisine bağlı damar endotel disfonksiyonuna katkısı olduğu düşünülmekle birlikte, resveratrolün söz konusu parametreler üzerindeki etkileri henüz araştırılmamıştır. Deneysel, prospektif, kontrollü çalışmada resveratrol uygulamasının sıçan torasik aortunda doksorubisinin oluşturduğu endotel fonksiyon değişiklikleri üzerine etkileri ve bu etkilerin NO sentezi ile ilişkisi incelenmiştir. Yöntemler: Wistar sıçanları 5 gruba ayrıldı; doksorubisin (n=9), çözücü (dimetilsülfoksit) (n=8), resveratrol (n=8), doksorubisin+resveratrol (n=10), kontrol (n=9). Gruplardan izole edilen torasik aort preparatlarında kasılma ve endotel aracılı/aracısız gevşeme yanıtları ile değişik NO-sentaz (NOS) izoformlarının ekspresyon düzeyleri histopatolojik olarak değerlendirildi. Doz-yanıt eğrilerinin istatistiksel değerlendirmesinde tekrarlayan ölçümler için ANOVA, eğrilerin pD2 ve Emax (maksimum fenilefrin kontraksiyonu) değerleri için ise tek yönlü ANOVA yöntemlerinin ardından Bonferroni testi uygulandı. Bulgular: Doksorubisin uygulaması (20 mg/kg, i.p.), endotelli preparatlarda fenilefrinle indüklenen kontraktil yanıtların (p

🔖

Cite This Article

OLUKMAN, M., ÇINAR, M. G., CAN, C., ORAL, O., EROL, A., ÖKTEM, G. (2022). Sıçan torasik aortunda doksorubisinin oluşturduğu fonksiyon bozukluğunun resveratrol uygulaması ile düzelmesi: Nitrik oksit sentaz inhibisyonunun olası rolü var mı?. Anadolu Kardiyoloji Dergisi, Cilt: 9 - Sayı: 4, 260–266. https://ajindex.com/articles/436912

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Lefrak EA, Pitha J, Rosenheim S, Gotlieb JA. A clinicopathologic analysis of adriamycin cardiotoxicity. Cancer 1973; 32: 302-14
  2. Bristow MR, Thompson PD, Martin RP, Mason JW, Billingham ME, Harrison DC. Early anthracycline cardiotoxicity. Am J Med 1978; 65: 823-32
  3. Murata T, Yamawaki H, Hori M, Ozaki H, Karaki H. Chronic vascular toxicity of doxorubicin in an organ-cultured artery. Br J Pharmacol 2001; 132: 1365-73
  4. Wu S, Ko Y, Teng MS, Ko YL, Hsu LA, Hsueh C, et al. Adriamycin-induced cardiomyocyte and endothelial cell apoptosis: in vitro and in vivo studies. J Mol Cell Cardiol 2002; 34: 1595-607
  5. Wolf MB, Baynes JW. The anti-cancer drug, doxorubicin, causes oxidant stress-induced endothelial dysfunction. Biochim Biophys Acta 2006; 1760: 267-71
  6. Vasquez-Vivar J, Martasek P, Hogg N, Masters BS, Pritchard KA Jr, Kalyanaraman B. Endothelial nitric oxide synthase-dependent superoxide generation from adriamycin. Biochemistry 1997; 36: 11293-7
  7. Duquaine D, Hirsch GA, Chakrabarti A, Han Z, Kehrer C, Brook R, et al. Rapid-onset endothelial dysfunction with adriamycin: evidence for a dysfunctional nitric oxide synthase. Vasc Med 2003; 8: 101-7
  8. Kalivendi WSV, Kotamraju S, Zhaao H, Joseph J, Kalyanaraman B. Doxorubicin-induced Apoptosis Is Associated with Increased Transcription of Endothelial Nitric-oxide Synthase. J Biol Chem 2001; 276: 47266-77
  9. Soleas GJ, Diamandis E, Goldberg DM. Resveratrol: a molecule whose time has come? And gone? Clin Biochem 1997; 30: 91-113
  10. Fremont L. Biological effects of resveratrol. Life Sci 2000; 66: 663-73
  11. Ray PS, Maulik G, Cordis GA, Bertelli AAE, Bertelli A, Das DK. The red wine antioxidant resveratrol protects isolated rat hearts from ischemia reperfusion injury. Free Radic Biol Med 1999; 27: 160-9
  12. Hattori R, Otani H, Maulik N, Das DK. Pharmacological preconditioning with resveratrol: a role of nitric oxide. Am J Physiol Heart Circ Physiol 2002; 282: H1988-95
  13. Rezk YA, Balulad SS, Keller RS, Bennett JA. Use of Resveratrol to improve the effectiveness of cisplatin and doxorubicin: Study in human gynecologic cancer cell lines and in rodent heart. Am J Obstet Gynecol 2006; 194: e23-e26
  14. Yu BB, Han XZ, Lou HX. Oligomers of resveratrol and ferulic acid prepared by peroxidase-catalysed oxidation and their protective effects on cardiac injury. J Agric Food Chem 2007; 55: 7753-7
  15. Nwankola RN, West WL. Inhibition of alpha-tocopherol and calcium calmodulin-stimulated phosphodiesterase a