Enterotoxin Production Potential of Bacillus Species Isolated from Ropy Bread

Objective & Hypothesis:In this study, Bacillus species isolated from ropy bread crumb in our previous study were investigated for the production of hemolytic (Hbl) and non-hemolytic (Nhe) enterotoxins.

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Key Findings & Data:Of the 39 isolates, only one produced both Hbl and Nhe enterotoxins, 22 produced only Hbl, 2 produced only Nhe and the remaining 14 isolates were found to produce none of the two enterotoxins.

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Conclusion & Impact:The results showed that Bacillus strains that are responsible for rope disease in bread could produce enterotoxins.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Abstract

In this study, Bacillus species isolated from ropy bread crumb in our previous study were investigated for the production of hemolytic (Hbl) and non-hemolytic (Nhe) enterotoxins. Of the 39 isolates, only one produced both Hbl and Nhe enterotoxins, 22 produced only Hbl, 2 produced only Nhe and the remaining 14 isolates were found to produce none of the two enterotoxins. Bacillus cereus 2248, which is known to produce both Hbl and Nhe, was used as the positive control strain for the enterotoxin assays. Toxin assays were also performed for the standard strains Bacillus subtilis PY22, B. subtilis RSK 244 and B. subtilis RSK 246. It was determined that amongst these strains only B. subtilis RSK 246 has the potential of producing the Nhe enterotoxin. Furthermore, the test results of Bacillus cereus 2455/2, which is known as an emetic strain, have shown that the strain was potentially positive in terms of Nhe enterotoxin. The results showed that Bacillus strains that are responsible for rope disease in bread could produce enterotoxins.

Özet

Bu çalışmada, daha önceki bir çalışmamızda sünme (rope) hastalığı oluşmuş ekmek içlerinden izole edilen Bacillustürleri, hemolitik (Hbl) ve hemolitik olmayan (Nhe) enterotoksin üretimleri açısından incelenmiştir. İncelenen toplam 39izolattan sadece birinin hem Hbl hem de Nhe enterotoksinlerini, 22 izolatın sadece Hbl, 2 izolatın ise sadece Nheenterotoksinini üretme kapasitesinin olduğu, kalan 14 izolatın ise her iki enterotoksini de üretmedikleri tespit edilmiştir.Enterotoksin analizleri için pozitif kontrol suşu olarak, her iki enterotoksini de ürettiği bilinen Bacillus cereus 2248kullanılmıştır. Toksin analizleri, standart suşlar olan Bacillus subtilis PY22, B. subtilis RSK 244 ve B. subtilis RSK 246için de yapılmış, aralarından sadece B. subtilis RSK 246’nın Nhe enterotoksinini üretme potansiyelinin olduğubelirlenmiştir. Ayrıca emetik toksin ürettiği bilinen Bacillus cereus 2455/2 suşu da Nhe enterotoksini açısından pozitifsonuç vermiştir. Sonuçlar, ekmeklerde sünme (rope) hastalığından sorumlu olan Bacillus türlerinin enterotoksinüretebileceğini gösterir niteliktedir.

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Cite This Article

EREM, F., CERTEL, M., BUDAK, B. K. (2022). Enterotoxin Production Potential of Bacillus Species Isolated from Ropy Bread. Akademik Gıda, Cilt: 18 - Sayı: 1, 64–72. https://ajindex.com/articles/432475

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References (13)

Schoeni, J.L., Wong, A.C.L. (2005). Bacillus cereus food poisoning and its toxins. Journal of Food Protection, 68(3), 636-648
Salkinoja-Salonen, M.S., Vuorio, R., Andersson, M.A., Kämpfer, P., Andersson, M.C., HonkanenBuzalski, T., Scoging, A.C. (1999). Toxigenic strains of Bacillus licheniformis related to food poisoning. Applied and Environmental Microbiology, 65(10), 4637-4645
Suominen, I., Andersson, M.A., Andersson, M.C., Hallaksela, A., Kämpfer, P., Rainey, F.A., Salkinoja-Salonen, M. (2001). Toxic Bacillus pumilus from indoor air, recycled paper pulp, Norway spruce, food poisoning outbreaks and clinical samples. Systematic and Applied Microbiology, 24, 267-276
From, C., Pukall, R., Schumann, P., Hormazábal, V., Granum, P.E. (2005). Toxin-producing ability among Bacillus spp. outside the Bacillus cereus group. Applied and Environmental Microbiology, 71(3), 1178-1183
Lindbäck, T., Granum, P.E. (2006). Detection and purification of Bacillus cereus enterotoxins. In Food Borne Pathogens: Methods and Protocols Edited by C.C. Adley, Humana Press, Totowa, 15-26p
Granum, P.E., Brynestad, S., O’sullivan, K., Nissen, H. (1993). Enterotoxin from Bacillus cereus: production and biochemical characterization. Netherlands Milk and Dairy Journal, 47, 63-70
Senesi, S., Ghelardi, E. (2010). Production, secretion and biological activity of Bacillus cereus enterotoxins. Toxins, 2, 1690-1703
Mckillip, J.L. (2000). Prevalence and expression of enterotoxins in Bacillus cereus and other Bacillus spp., a literature review. Antonie von Leeuwenhoek, 77, 393-399
Cai, Y., Huang, T., Xu, Y., Zhou, G., Zou, P., Zeng, G., Liu, X. (2017). Genetic and genomic diversity of NheABC locus from Bacillus strains. Archieves of Microbiology, 199, 775-785
Agata, N., Ohta, M., Arakawa, Y., Mori, M. (1995). The bceT gene of Bacillus cereus encodes an enterotoxic protein. Microbiology, 141, 938-988
Choma, C., Granum, P.E. (2002). The enterotoxin T (BcET) from Bacillus cereus can probably not contribute to food poisoning. FEMS Microbiology Letters, 217(1), 115-119
Hansen, B.M., Høiby, P.E., Jensen, G.B., Hendriksen, N.B. (2003). The Bacillus cereus bceT enterotoxin sequence reappraised. FEMS Microbiology Letters, 223, 21-24
Asano, S.I., Nukumizu, Y., Bando, H., Iizuka, T., Yamamoto, T. (1997). Cloning of novel enterotoxin genes from Bacillus cereus and Bacillus thuringiensis. Applied and Environmental Microbiology, 63(3), 1054-10