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Journals / Akademik Gıda / 2020 / Cilt: 18 - Sayı: 3

Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method

Ultrason Destekli Ekstraksiyon Yöntemi ile Elde Edilen Violasinin Antibakteriyel ve Antikanser Aktiviteleri

Seyhan Ulusoy
Çağdaş Deniz Periz
Turgut Şekerler
Gülgün Tınaz
Pages
241–246
Publication Date
2022-10-11
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was found out thatviolacein extracts had an antibacterial effect against Staphylococcus aureus, S. aureus (MRSA) and Bacillus cereus.The violacein extract had a strong anticancer effect on cervical cancer (HeLa) cells.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Violacein is a natural violet pigment produced by various bacterial species, especially by the Gram-negative bacteriaChromobacterium violaceum.
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Key Findings & Data:Violacein was quantified usingHigh Performance Liquid Chromatography (HPLC).
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Conclusion & Impact:It was found out thatviolacein extracts had an antibacterial effect against Staphylococcus aureus, S. aureus (MRSA) and Bacillus cereus.The violacein extract had a strong anticancer effect on cervical cancer (HeLa) cells.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Violacein is a natural violet pigment produced by various bacterial species, especially by the Gram-negative bacteriaChromobacterium violaceum. Violacein has antibacterial, antifungal, antioxidant and anticancer activities and hasvarious applications in food, cosmetic, textile and pharmaceutical industries. In this study, violacein was extractedfrom the Chromobacterium violaceum culture through ultrasonic-assisted (UA) and microwave-assisted (MA)extraction methods using methanol, ethanol, acetone, and ethyl acetate as solvent. Violacein was quantified usingHigh Performance Liquid Chromatography (HPLC). Then, antibacterial and anticancer activities of these extracts wereinvestigated. In this study, for the first time, the violacein extract was obtained by using UA and MA extractionmethods. The highest violacein concentration could be achieved by UAE method with methanol. It was found out thatviolacein extracts had an antibacterial effect against Staphylococcus aureus, S. aureus (MRSA) and Bacillus cereus.The violacein extract had a strong anticancer effect on cervical cancer (HeLa) cells. Finally, the UA and MA extractionmethods were successfully applied to bacterial cultures in order to extract the violacein.

Özet

Viyolasin, özellikle Gram-negatif bakteri Chromobacterium violaceum tarafından üretilen ve antibakteriyel, antifungal, antioksidan ve antikanser etkilere sahip çeşitli bakteri türleri tarafından üretilen doğal mor bir pigmenttir. Bu çalışmada, viyolasin, Ultrason destekli (UA) ve mikrodalga destekli (MA) ekstraksiyon prosedürleri ile C. violaceum suşundan metanol, etanol, aseton ve etil asetat kullanılarak ekstrakte edilmiştir. Elde edilen viyolasin miktarı, yüksek performanslı sıvı kromatografisi (HPLC) kullanılarak belirlenmiştir. Daha sonra bu ekstraktların antibakteriyel ve antikanser aktiviteleri incelenmiştir. Bu çalışmada, viyolasin ilk kez UA ve MA ekstraksiyon yöntemleri kullanılarak elde edilmiştir. Viyolasin ekstrelerinin Staphylococcus aureus, S. aureus (MRSA) ve Bacillus cereus'a karşı antibakteriyel aktiviteye sahip olduğu belirlenmiştir. Viyolasin ekstresi rahim ağzı kanseri hücreleri (HeLa) üzerinde güçlü anti-kanser etkisi göstermiştir. Sonuçta, viyolasini ekstrakte etmek için, UA ve MA ekstraksiyon yöntemleri bakteri kültürlerine başarıyla uygulanmıştır.

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Cite This Article

Ulusoy, S., Periz, Ç. D., Şekerler, T., Tınaz, G. (2022). Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method. Akademik Gıda, Cilt: 18 - Sayı: 3, 241–246. https://ajindex.com/articles/432471

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