SavedAdd your journal

Journals / Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi / 2018 / Cilt: 18 - Sayı: 3

In Vitro Investigation of the Anticancer Activity of Friedelin in Glioblastoma Multiforme

Glioblastoma Multiformede Friedelinin Antikanser Aktivitesinin In Vitro İncelenmesi

Bugrahan EMSEN
Hasan TÜRKEZ
Tubanur ENGİN
Pages
763–773
Publication Date
2022-10-12
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Consequently, the results obtained from the present study proved thepotential of natural product with an antioxidant capacity as a source for anticancer compound againstGBM.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:There are different herbal methods used for support in many cancer diseases.
📊
Key Findings & Data:It was used human U87MG-GBM cancer cell lines and primaryrat cerebral cortex (PRCC) non-cancerous cells isolated from Sprague-Dawley rats in order to determineside effect level of FRI.
💡
Conclusion & Impact:Consequently, the results obtained from the present study proved thepotential of natural product with an antioxidant capacity as a source for anticancer compound againstGBM.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

There are different herbal methods used for support in many cancer diseases. Lichens are importantorganisms containing unique herbal compounds and it is known that they have different anticanceractivities. Starting from these features, the present study was aimed to investigate anticancer activity offriedelin (FRI), a lichen compound against glioblastoma multiforme (GBM) showed dangerous malignantproperties within brain cancer species. It was used human U87MG-GBM cancer cell lines and primaryrat cerebral cortex (PRCC) non-cancerous cells isolated from Sprague-Dawley rats in order to determineside effect level of FRI. In the experiments, cytotoxic (via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) tests), antioxidant (via totalantioxidant capacity (TAC) test), pro-oxidant (via total oxidative stress (TOS) test) and genotoxic (via 8-hydroxy-2′-deoxyguanosine (8-OH-dG) test) activities of different concentrations of FRI were tested. Asa result of the study, MTT assay revealed that FRI showed higher cytotoxic activity on U87MG cellscompared to PRCC cells (median inhibitory concentration (IC 50 ): 46.38 and 1271.77 mg/L, respectively).Based on U87MG cells, it was determined a significant positive correlation between LDH and TOSactivities. High positive correlation between TAC and cell viability on healthy PRCC cells exhibitedantioxidant capacity of FRI. Consequently, the results obtained from the present study proved thepotential of natural product with an antioxidant capacity as a source for anticancer compound againstGBM.

Özet

Pek çok kanser hastalığında destek için kullanılan farklı bitkisel yöntemler vardır. Likenler benzersiz bitkisel bileşikler içeren önemli organizmalardır ve farklı antikanser aktivitelerine sahip oldukları bilinmektedir. Bu özelliklerden yola çıkarak, mevcut çalışma, beyin kanseri türleri içerisinde tehlikeli habis özellikler gösteren glioblastoma multiformeye (GBM) karşı bir liken bileşiği olan friedelinin (FRI) antikanser aktivitesini araştırmayı amaçlamıştır. FRI'nın yan etki düzeyini belirlemek için insan U87MG- GBM kanser hücre hatları ve Sprague-Dawley sıçanlarından izole edilen primer sıçan serebral korteks (PSSK) kanserli olmayan hücreler kullanılmıştır. Denemelerde, FRI'nın farklı konsantrasyonlarının sitotoksik (3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür (MTT) ve laktat dehidrogenaz (LDH) testleri ile), antioksidan (toplam antioksidan kapasite (TAK) testi ile), pro-oksidan (toplam oksidatif stres (TOS) ile) ve genotoksik (8-hidroksi-2′-deoksiguanozin (8-OH-dG) testi ile) aktiviteleri test edilmiştir. Çalışma sonucunda MTT uygulaması, FRI'nın PSSK hücrelerine kıyasla U87MG hücreleri üzerinde daha yüksek sitotoksik aktivite gösterdiğini ortaya koymuştur (sırasıyla medyan inhibitör konsantrasyonu (IC 50 ): 1271.77 ve 46.38 mg/L). U87MG hücreleri temel alındığında, LDH ve TOS aktiviteleri arasında anlamlı bir pozitif korelasyon olduğu saptanmıştır. Sağlıklı PSSK hücrelerinde, TAK ve hücre canlılığı arasındaki yüksek pozitif korelasyon FRI'nın antioksidan kapasitesini sergilemiştir. Sonuç olarak, mevcut çalışmadan elde edilen sonuçlar, GBM'ye karşı antikanser bileşiği kaynağı olarak antioksidan kapasiteye sahip doğal ürün potansiyelini ortaya koymuştur.

🔖

Cite This Article

EMSEN, B., TÜRKEZ, H., ENGİN, T. (2022). In Vitro Investigation of the Anticancer Activity of Friedelin in Glioblastoma Multiforme. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, Cilt: 18 - Sayı: 3, 763–773. https://ajindex.com/articles/428520

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (8)

  1. Abuiraq, L., Kanan, G., Wedyan, M. and El-Oqlah, A., 2015. Efficacy of extracts of some lichens for potential antibacterial activity. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6, 318–331.
  2. Alpsoy, L., Aslan, A., Kotan, E., Agar, G. and Anar, M., 2011. Protective role of two lichens in human lymphocytes in vitro. Fresenius Environmental Bulletin, 20, 1661–1666.
  3. Ari, F., Celikler, S., Oran, S., Balikci, N., Ozturk, S., Ozel, M.Z., Ozyurt, D. and Ulukaya, E., 2014. Genotoxic, cytotoxic, and apoptotic effects of Hypogymnia physodes (L.) Nyl. on breast cancer cells. Environmental Toxicology, 29, 804–813.
  4. Bai, L., Bao, H.Y. and Bau, T., 2014. Isolation and identification of a new benzofuranone derivative from Usnea longissima. Natural Product Research, 28, 534–538. Bézivin, C., Tomasi, S., Lohézic-Le Dévéhat, F. and Boustie, J., 2003. Cytotoxic activity of some lichen extracts on murine and human cancer cell lines. Phytomedicine, 10, 499–503. Bézivin, C., Tomasi, S., Rouaud, I., Delcros, J.G. and Boustie, J., 2004. Cytotoxic activity of compounds from the lichen: Cladonia convoluta. Planta Medica, 70, 874–877.
  5. Bosset, J.-F., Collette, L., Calais, G., Mineur, L., Maingon, P., Radosevic-Jelic, L., Daban, A., Bardet, E., Beny, A., Ollier, J.-C. and EORTC Radiotherapy Group Trial 22921, 2006. Chemotherapy with preoperative radiotherapy in rectal cancer. The New England Journal of Medicine, 355, 1114–1123.
  6. Boustie, J. and Grube, M., 2005. Lichens-A promising source of bioactive secondary metabolites. Plant Genetic Resources, 3, 273– 287. Brisdelli, F., Perilli, M., Sellitri, D., Piovano, M., Garbarino, J.A., Nicoletti, M., Bozzi, A., Amicosante, G. and Celenza, G., 2013. Cytotoxic activity and antioxidant capacity of purified lichen metabolites: an in vitro study. Phytotherapy Research, 27, 431–437.
  7. Brodo, I.M., Sharnoff, S.D. and Sharnoff, S., 2001. About the Lichens, in: Lichens of North America. Yale University Press, New Haven & London, 3– 113. Clarke, J., Penas, C., Pastori, C., Komotar, R.J., Bregy, A., Shah, A.H., Wahlestedt, C. and Ayad, N.G., 2013. Epigenetic pathways and glioblastoma treatment. Epigenetics, 8, 785– 795. Çelikler Kasımoğulları, S., Oran, S., Ari, F., Ulukaya, E., Aztopal, N., Sarimahmut, M. and Öztürk, Ş., 2014. Genotoxic, cytotoxic, and apoptotic effects of crude extract of Usnea filipendula Stirt. in vitro. Turkish Journal of Biology, 38, 940–947.
  8. Dogan, M., Emsen, B., Aasim, M. and Yildirim, E., 2017. Ceratophyl