Journals / Harran Tarım ve Gıda Bilimleri Dergisi / 2020 / Cilt: 24 - Sayı: 1

Antifungal effect of boric acid against Penicillium expansum, the casual agent of blue mold of apple

Elmada mavi küfe neden olan Penicillium expansum’a karşı borik asitin antifungal etkisi

Pages
64–72
Publication Date
2020-03-20
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results indicate that the boric acid can be used as an alternative to synthetic fungicides for the control of blue mold disease caused by P. expansum in apples.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Efficacy of boric acid against Penicillium expansum was evaluated under both in vitro and in vivo conditions in this study.
📊
Key Findings & Data:In in vitro, 0.125% concentration of boric acid reduced mycelial growth, spore germination and germ tube elongation of P. expansum by 68.88%, 74.00% and 85.50% respectively, whereas the higher concentrations (0.25%, 0.50%, 1.00% and 2.00%) of boric acid completely inhibited those of P. expansum.
💡
Conclusion & Impact:These results indicate that the boric acid can be used as an alternative to synthetic fungicides for the control of blue mold disease caused by P. expansum in apples.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Elma · Mavi küf · Borik asit

Abstract

Efficacy of boric acid against Penicillium expansum was evaluated under both in vitro and in vivo conditions in this study. In in vitro, 0.125% concentration of boric acid reduced mycelial growth, spore germination and germ tube elongation of P. expansum by 68.88%, 74.00% and 85.50% respectively, whereas the higher concentrations (0.25%, 0.50%, 1.00% and 2.00%) of boric acid completely inhibited those of P. expansum. Moreover, the difference among the effects of the above-mentioned first concentration and subsequent concentrations of boric acid was statistically significant (P<0.05). The EC50, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of boric acid were defined as 0.09, 0.25% and >2%, respectively. In in vivo experiments, except for 0.25% boric acid, all the other concentrations (0.50%, 1.00% and 2.00%) exhibited both protective and curative activity against P. expansum, and they significantly reducing the disease severity of blue mold in comparison to pathogen‐inoculated control (P<0.05). However, 2.00% boric acid has been shown to be the most effective concentration against the pathogen in both protective and curative applications, and reduce the disease severity by 94.46% and 91.41%, respectively. These results indicate that the boric acid can be used as an alternative to synthetic fungicides for the control of blue mold disease caused by P. expansum in apples.

Özet

Bu çalışmada, borik asitin Penicillium expansum’a karşı etkinliği hem in vitro hem de in vivo koşullarda değerlendirilmiştir. In vitro’da, borik asitin %0.125 konsantrasyonu P. expansum’un misel gelişmesini, spor çimlenmesini ve çim tüpü uzunluğunu sırasıyla %68.88, %74.00 ve %85.50 oranında azaltmış, halbuki daha yüksek konsantrasyonlar (%0.25, %0.50, %1.00 ve %2.00)’ı ise tamamen engellemiştir. Ayrıca borik asitin yukarıda belirtilen ilk konsantrasyonu ve sonraki konsantrasyonlarının etkileri arasındaki fark istatistiksel olarak önemli bulunmuştur (P<0.05). Borik asitin EC50, minimum engelleyici konsantrasyon (MIC) ve minimum fungisidal konsantrasyon (MFC) değerleri sırasıyla, 0.09, %0.25 ve >%2 olarak belirlenmiştir. In vivo denemelerde, borik asitin %0.25 konsantrasyonu hariç, diğer tüm konsantrasyonlar (%0.50, %1.00 ve %2.00)’ı hem koruyucu hem de tedavi edici etki göstermişler ve bu konsantrasyonlar mavi küfün hastalık şiddetini patojen ile inokule edilmiş kontrole kıyasla önemli ölçüde azaltmıştır (P<0.05). Bununla birlikte, %2.00 borik asitin hem koruyucu hem de tedavi edici uygulamalarda patojene karşı en etkili konsantrasyon olduğu ve hastalık şiddetini sırasıyla %94.46 ve %91.41 oranında azalttığı gösterilmiştir. Bu sonuçlar, borik asitin elmalarda P. expansum'un neden olduğu mavi küf hastalığının mücadelesi için sentetik fungisitlere bir alternatif olarak kullanılabileceğini göstermektedir.

🔖

Cite This Article

YILDIRIM, E., KARATOPRAK, K., ERPER, İ., TÜRKKAN, M. (2020). Antifungal effect of boric acid against Penicillium expansum, the casual agent of blue mold of apple. Harran Tarım ve Gıda Bilimleri Dergisi, Cilt: 24 - Sayı: 1, 64–72. https://ajindex.com/articles/423237

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Agrios, G. N. (2005). Plant pathology. Academic press,. Akhtar, K. P., Matin, M., Mirza, J. H., Shakir, A. S., & Rafique, M. (1994). Some studies on the post harvest diseases of tomato fruits and their chemical control. Pak. J. Phytopathol, 6, 125-129. Anonymous (2019a). Bitki koruma ürünleri veri tabanı (BKU). https://bku.tarim.gov.tr. (Erişim tarihi: 15 Ocak 2019). Anonymous (2019b). https://www.enerji.gov.tr/tr-TR/Sayfalar/Bor (Erişim tarihi: 4 Mart 2019).
  2. Cao, B., Li, H., Tian, S., & Qin, G. (2012). Boron improves the biocontrol activity of Cryptococcus laurentii against Penicillium expansum in jujube fruit. Postharvest Biology and Technology, 68, 16-21.
  3. Conway, W. S., Leverentz, B., Janisiewicz, W. J., Blodgett, A. B., Saftner, R. A., & Camp, M. J. (2004). Integrating heat treatment, biocontrol and sodium bicarbonate to reduce postharvest decay of apple caused by Colletotrichum acutatum and Penicillium expansum. Postharvest Biology and Technology, 34(1), 11-20.
  4. Conway, W. S., Leverentz, B., Janisiewicz, W. J., Saftner, R. A., & Camp, M. J. (2005). Improving biocontrol using antagonist mixtures with heat and/or sodium bicarbonate to control postharvest decay of apple fruit. Postharvest Biology and Technology, 36(3), 235-244.
  5. Droby, S., Wisniewski, M., El Ghaouth, A., & Wilson, C. (2003). Influence of food additives on the control of postharvest rots of apple and peach and efficacy of the yeast-based biocontrol product Aspire. Postharvest Biology and Technology, 27(2), 127-135. FAO, (2019). http://www.fao.org/faostat/en/#data/QC (Erişim tarihi: 18.01.2019)
  6. Grant, I. R., & Patterson, M. F. (1991). Effect of irradiation and modified atmosphere packaging on the microbiological safety of minced pork stored under temperature abuse conditions. International journal of food science & technology, 26(5), 521-533.
  7. Janisiewicz, W. J. (1998). Biocontrol of postharvest diseases of temperate fruits. Plant-microbe interactions and biological control, 171-198.
  8. Karabulut, O. A., Arslan, U., İlhan, K., & Kuruoğlu, G. (2005). Integrated control of postharvest diseases of sweet cherry with yeast antagonists and sodium bicarbonate applications within a hydrocooler. Postharvest biology and technology, 37(2), 135-141.
  9. Li, Y., Yang, Z., Bi, Y., Zhang, J., & Wang, D. (2012). Antifungal effect of borates against Fusarium sulphureum on potato tubers and its possible mechanisms of action. Postharvest biology and technology, 74, 55-61.
  10. Mari, M., Leoni, O., Iori, R., & Cembali, T. (