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Journals / Harran Tarım ve Gıda Bilimleri Dergisi / 2017 / Cilt: 21 - Sayı: 2

MicroRNA-Based Interference Applications in Plant Biotechnology

Bitki Biyoteknolojisi’nde MikroRNA Tabanlı İnterferans Uygulamaları

Fatma AYDINOĞLU
Gizem AKTUĞ
Pages
227–238
Publication Date
2017-06-19
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This review aims to provide the latest updates on plant miRNAs and applications of miRNA-based RNAi technology for crop improvement.

📌 3 Key Takeaways

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Objective & Hypothesis:Plant biotechnology aims to improve desirable characters of plants by using modern genetic engineering tools.
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Key Findings & Data:Recently, RNA interference (RNAi), which is a natural sequence-specific gene expression regulatory mechanism involving non-coding small RNAs like microRNA (miRNA), has gained importance as a beneficial tool for crop improvement.
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Conclusion & Impact:This review aims to provide the latest updates on plant miRNAs and applications of miRNA-based RNAi technology for crop improvement.
🔬Design:Review & Systematic Survey
📍Scope:Comprehensive Sample
⚙️Variables:Bitki biyoteknolojisi · miRNA · RNAi

Abstract

Plant biotechnology aims to improve desirable characters of plants by using modern genetic engineering tools. Recently, RNA interference (RNAi), which is a natural sequence-specific gene expression regulatory mechanism involving non-coding small RNAs like microRNA (miRNA), has gained importance as a beneficial tool for crop improvement. MiRNAs are identified as the key regulators in almost all biological and metabolic processes. Hereby, manipulating miRNA-based RNAi pathways offer a promising tool in the presence of successful applications on several fields of plant biotechnology, such as altering plant architecture, improving abiotic stress tolerance and biotic stress resistance, enrichment the nutritional value of plants, prolonging shelf life of fruits and vegetables, enhancing secondary metabolite production. This review aims to provide the latest updates on plant miRNAs and applications of miRNA-based RNAi technology for crop improvement.

Özet

Bitki biyoteknolojisi, modern genetik mühendisliği araçlarını kullanarak bitkilerin istenilen karakterlerinin iyileştirilmesini amaçlar. Son yıllarda, protein kodlamayan küçük RNA’lardan olan mikroRNA (miRNA) genlerinin yer aldığı, doğal gen ifadesini düzenleyici mekanizma olan RNA interferans (RNAi), bitki geliştirilmesinde faydalı bir araç olarak önem kazanmıştır. miRNA’ların, hemen hemen bütün biyolojik ve metabolik işlevde anahtar düzenleyici role sahip oldukları ortaya konulmuştur. Hücresel yolakların miRNA tabanlı RNAi ile manipüle edilmesi ile bitki yapısının değiştirilmesi, abiyotik streslere toleransın ve biyotik streslere direncin geliştirilmesi, bitkilerin besin değerlerince zenginleştirilmesi, meyve ve sebzelerde raf ömrünün uzatılması ve sekonder metabolit üretiminin artırılması gibi daha pek çok bitki biyoteknolojisi alanında başarılı örneklerin varlığı, bu teknolojinin gelecek vaat eden bir araç olduğunu göstermektedir. Bu bağlamda, bu derleme, bitki miRNA genleri ve miRNA tabanlı RNAi teknolojisinin bitki iyileştirilmesinde uygulamalarına dair son gelişmeleri sunmayı amaçlamaktadır.

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Cite This Article

AYDINOĞLU, F., AKTUĞ, G. (2017). MicroRNA-Based Interference Applications in Plant Biotechnology. Harran Tarım ve Gıda Bilimleri Dergisi, Cilt: 21 - Sayı: 2, 227–238. https://ajindex.com/articles/423079

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