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Journals / Turkish Journal of Pharmaceutical Sciences / 2006 / Cilt: 3 - Sayı: 1

Farklı klinik örneklerden izole edilen Candida türlerinin identifikasyonu için CHROMmagar-Candida'nın izolasyon besiyeri olarak değerlendirilmesi

The evaluation of CHROMagar-Candida as an isolation medium for identification of Candida species isolated from different clinical specimens

BERRİN ÖZÇELİK
Pages
41–47
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The use of CHROMagar as a yeast isolation medium in a clinical laboratory for the routine examination appears to be extremely successful in primary isolation and differentiation medium in different sample collections (vaginal specimens, blood cultures, gaita, urine sample, sputum and oropharyngeal swabs) of yeast also it is more rapidly than classical methods.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of the study was to evaluate the accuracy of CHROMagar-Candida for the identification of Candida spp. from different clinical specimens.
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Key Findings & Data:Non-Candida albicans yeast isolates were further specificated with API 20C (bioMerieux, France).
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Conclusion & Impact:The use of CHROMagar as a yeast isolation medium in a clinical laboratory for the routine examination appears to be extremely successful in primary isolation and differentiation medium in different sample collections (vaginal specimens, blood cultures, gaita, urine sample, sputum and oropharyngeal swabs) of yeast also it is more rapidly than classical methods.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Candida · Mikrobiyolojik teknikler · Mikolojik tiplendirme teknikleri

Abstract

The aim of the study was to evaluate the accuracy of CHROMagar-Candida for the identification of Candida spp. from different clinical specimens. The growth of cultures on plates, containing Sabouraud dextrose agar and CHROMagar-Candida were compared. Non-Candida albicans yeast isolates were further specificated with API 20C (bioMerieux, France). Of the 178 cultures processed, 100% accuracy for C. albicans C. krusei, C. tropicalis and 99% for other Candida spp. were determined. CHROMagar-Candida was found to be a satisfactory isolation medium for different clinical specimens, allowing immediate and correct identification of the commonly encountered yeasts. The use of CHROMagar as a yeast isolation medium in a clinical laboratory for the routine examination appears to be extremely successful in primary isolation and differentiation medium in different sample collections (vaginal specimens, blood cultures, gaita, urine sample, sputum and oropharyngeal swabs) of yeast also it is more rapidly than classical methods.

Özet

Çalışma farklı klinik örneklerden izole edilen Candida türlerinin identifıkasyonu için CHROMagar-Candida'nın izolasyon besiyeri olarak doğruluğunun değerlendirilmesi amacıyla yapılmıştır. Kültürlerin Saboraud Dextrose Agar ve CHROMagar-Candida besiyerleri içeren petri plaklarındaki üremesi birbiri ile kıyaslanmıştır. Non-Candida albicans maya izolatları API20C (bioMerieux, France) ile ileri incelemeye alınmıştır. İşleme tabi tutulan 178 kültürde; C. albicans, C. krusei, C. tropicalis için %100 ve diğer Candida spp. için %99 uyum saptanmıştır. Sıklıkla karşılaşılan maya türleri için hızlı ve doğru identifıkasyon yapan CHROMagar-Candida, değişik klinik örnekler için uygun bir besiyeri olarak bulunmuştur. CHROMagar 'in klinik mikrobiyoloji laboratuvarlarında maya izolasyon besiyeri olarak rutin tanı amacıyla kullanılması değişik örneklerde (vajinal örnekler, kan kültürü, gaita, idrar, balgam ve orofarengeal sürüntü) mayaların ilk izolasyonu için oldukça başarılı sonuç vermektedir ve aynı zamanda klasik metodlara göre çok daha hızlıdır.

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Cite This Article

ÖZÇELİK, B. (2006). Farklı klinik örneklerden izole edilen Candida türlerinin identifikasyonu için CHROMmagar-Candida'nın izolasyon besiyeri olarak değerlendirilmesi. Turkish Journal of Pharmaceutical Sciences, Cilt: 3 - Sayı: 1, 41–47. https://ajindex.com/articles/404067

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