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Journals / Turkish Journal of Pharmaceutical Sciences / 2004 / Cilt: 1 - Sayı: 3

Antitumoral aktivite tarama testi olarak, bazı ajanların Vero, He-La ve Hep-2 hücre kültürlerinde sitotoksisitelerinin değerlendirilmesi

Evaluation of cytotoxicity of some agents as a screening test in antitumoral activity using Vero, He-La and Hep-2 cell cultures

BERRİN ÖZÇELİK
MEHMET TANER KARAOĞLU
Ufuk ABBASOĞLU
Pages
153–163
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Cell culture tests can be used as a screening test in antitumoral activity screening for the small quantity of synthesized and expensive materials.

📌 3 Key Takeaways

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Objective & Hypothesis:Tissue culture tests which presents in the in-vitro antitumoral activity tests that permit to investigate thoroughly the different effects of the compounds in specific doses which interacts with DNA have been researched by many authors.
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Key Findings & Data:In this study, from the antitumoral and antifungal agents namely, bleomicyn, mitomycin C, daunorubicin, cyclophosphamide, iphosphamide, vinorelbine, vinblastine, gemcitabine, cytarabine, carboplatin, cisplatin, flukonazole, and terbinafine were investigated by using Vero, He-La and Hep-2 cell cultures.
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Conclusion & Impact:Cell culture tests can be used as a screening test in antitumoral activity screening for the small quantity of synthesized and expensive materials.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Antineoplastik ajanlar · Sitotoksisite · immünolojik

Abstract

Tissue culture tests which presents in the in-vitro antitumoral activity tests that permit to investigate thoroughly the different effects of the compounds in specific doses which interacts with DNA have been researched by many authors. In this study, from the antitumoral and antifungal agents namely, bleomicyn, mitomycin C, daunorubicin, cyclophosphamide, iphosphamide, vinorelbine, vinblastine, gemcitabine, cytarabine, carboplatin, cisplatin, flukonazole, and terbinafine were investigated by using Vero, He-La and Hep-2 cell cultures. The cytopathogenic effects of tested materials were evaluated by cell culture microscopy. While the maximum cytotoxicity was determined in daunorubicin in minimal dose at 0.098µg/ml, lower effect was determined in vincristine, mitomycin C, bleomicyn, gemcitabine, carboplatin, cytarabine, cisplatin, and vinblastine, and cytarabine in Vero, He-La and Hep-2 cell line cultures. No activities were observed with antifungal agents. When the results were compared with the reports, the different cytotoxic activity in different cells and different doses may result to miss the possible antitumoral effect. For this reason, several cell line cultures have to be used. Cell culture tests can be used as a screening test in antitumoral activity screening for the small quantity of synthesized and expensive materials.

Özet

DNA ile etkileşen bileşiklerin belirli dozlarda gösterdiği farklı etkilerin çok yönlü incelenmesine olanak tanıyan in-vitro antitumoral aktivite testleri arasında yer alan hücre kültürü testleri; birçok araştırıcı tarafından denenmektedir. Çalışmada; antitumoral ve antifungal ajanlardan bleomisin, mitomisin, daunorubisin, siklofosfamid, ifosfamid, vinblastin, vinorelbin, gemistabin, sitarabin, karboplatin, sisplatin, flukonazol ve terbinafin, Vero, He-La ve Hep-2 hücre kültürlerinde araştırılmıştır. Sitopatojenik etki doku kültürü mikroskobu ile değerlendirilmiştir. Vero, He-La ve Hep-2 hücre kültüründe en düşük dozda 0.098µg/ml’de en yüksek sitotoksisite daunorubisinde görülürken, bununla kıyasla daha az etki vinkristin, mitomisin C, bleomisin, gemsitabin, karboplatin, siderabin,cisplatin,vevinblastin’ de görülmüştür. Antifungal ajanlarda sitotoksik etki görülmemiştir. Yapılmış araştırmalarla kıyaslanan sonuçlarda maddelerin farklı hücrelerde farklı dozlarda sitotoksik etki göstermesi, olası antitumoral etkinin gözden kaçmasına neden olabileceğini ortaya koymuştur. Bu nedenle çeşitli hücre kültürlerinin kullanılması gereği görülmektedir. Miktarı az ve maliyeti yüksek olan maddeler için aynı zamanda hassas sonuçlar vermesi nedeniyle hücre kültürü testi antitumoral aktivite taramalarında ön tarama testi olarak seçilebilir.

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Cite This Article

ÖZÇELİK, B., KARAOĞLU, M. T., ABBASOĞLU, U. (2004). Antitumoral aktivite tarama testi olarak, bazı ajanların Vero, He-La ve Hep-2 hücre kültürlerinde sitotoksisitelerinin değerlendirilmesi. Turkish Journal of Pharmaceutical Sciences, Cilt: 1 - Sayı: 3, 153–163. https://ajindex.com/articles/403897

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References (12)

  1. Wolff, B., Targeting of cytotoxic principles to tumor cells. In: Microbiological Model as Screening Tools for Anticancer Agents; Potentials and Limitations. Eds: F.M.J., Debruyne, J.A., Schalken, Ann.Rev.Microbiol., CRC Press, pp. 415-33, 36, 1982
  2. Kinghorn, A.D., Cui, B., Ito, A., Chung, H.S., Long, L., Chang, L.C., Fractionation of plants to discover substances to combat cancer. In: Biologically Active Natural Products, Eds: S., Cutler, H., Cutler, CRC Press, London, pp. 43-57, 2000
  3. Hanauske, A., Hilsenbeck, S.G., Hoff, D., Human tumor screening. In: Anticancer Drug Development Guide, Ed: B.A., Teicher,, Humana Press Ins., New Jersey, pp.43-55,1997
  4. Corbett, T., Valeriote, F., LoRusso, P., Polin, L., Panchapor, C., Pugh, S., In-vivo metods for screeninng and preclinical testing. In: Anticancer Drug Development Guide Ed.: B.A., Teicher, Humana Press, New Jersey, pp.75-99,1997
  5. Calabresi, P., Chabner, B.A., Chemotherapy of neoplastic disease. In: The Pharmacological Basis of Therapeutics, Eds: A.G., Gilman, S., Rall, AS., Nies, P., Taylor, pp. 1202-62, 1991
  6. Boyd, M.R., The NCI in-vitro anticancer drug discovery screen. In: Anticancer Drug Development Guide, Ed. B.A., Teicher, Humana Press Ins., pp. 23-43, 1997
  7. Jacqueline, C., Colin, B.A., Reid, Marianne, L.T., Hilde, T., Charles, R.L., Gordon, B.S., Boudewıjn, J.M., “A comparison of bleomicin induced damage in lymphocytes and primary oral fibroblasts and keratinocytes in 30 Subjects” Mutagenesis, 14, 87-93, 1999
  8. Nesslany Corbet, T.H., Leopold, W.R., Dykes, J.D., Roberts, B.J., Griswold, D.P., Schabel, F.M., “Toxicity and anticancer activity of new triazine antifolate” Cancer.Res, 42, 1707-1715, 1882
  9. Crook, T.R., Souhami, R.L., Mclean, A.E., “Cytotoxicity, DNA cosslinking and single strand break induced by activated cyclophosphamide and acrolein in human leukemia cells” Cancer.Res., 46, 5029-34, 1986
  10. Esquenazi, D., Wigg, M.D., Miranda, M.F.S, Rodrigues, H.M., Tostes, B.F., Rozental, S., Silva, J.R, Alviano, C.S., “Antimicrobial and antiviral activities of polyphenolics from Cocos nucifera Linn.(Palmae) husk fiber extract” Research in Microbiology, 153, 647-652, 2002
  11. Cooper, J.K., Goldstein, S., “Toxicity testing in-vitro. use of microsome cultured human fibroblast system to study the cytotoxicity of cyclophosphamide” Cancer J.Physiol., 54, 4, 546-50, 1976
  12. Westendorf, J., Marquat, H., “Structure-activity relationship of anthracycline-induced genotoxicity in-vit