Journals / Turkish Journal of Pharmaceutical Sciences / 2004 / Cilt: 1 - Sayı: 2

Stafilokoklarda sıvı mikrodilüsyon ve disk difüzyon yöntemlerinin kıyaslanmasıyla duyarlılık testlerinin değerlendirilmesi

Evaluation of susceptibility testing by comparison of broth microdilution and disk agar diffusion tests in staphylococci

Pages
77–86
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

After comparison of the two methods results according to NCCLS criteria, in the 65 strains 97.5 % correlation was determined.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:There are methods for susceptibility testing for microorganisms that are isolated from different clinical samples.
📊
Key Findings & Data:Accurate breakpoint of Staphylococcus aureus is crucial in the management and treatment of both colonized and infected patients.
💡
Conclusion & Impact:After comparison of the two methods results according to NCCLS criteria, in the 65 strains 97.5 % correlation was determined.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Mikrobiyal duyarlılık testleri · Penisilinler · Trimetoprim-sulfametoksazol kombinasyonu

Abstract

There are methods for susceptibility testing for microorganisms that are isolated from different clinical samples. In order to obtain reliable results, many researchers have been working on the sensitivity of susceptibility tests and comparing the different methods. Staphylococci are important bacteria that commonly come out as causative agents in many infections with both hospital and community origin. Accurate breakpoint of Staphylococcus aureus is crucial in the management and treatment of both colonized and infected patients. To determine the in-vitro activity of trimethoprim-sulphamethoxazole, penicillin, tetracycline, vancomycine and erythromycin agents against S.aureus, isolates were collected from Naşide Halil Gelendost Primary School students of ages 7-12 were studied with microdilution and disk diffusion method. After comparison of the two methods results according to NCCLS criteria, in the 65 strains 97.5 % correlation was determined.

Özet

Klinik örneklerden izole edilen suşların duyarlılıklarının araştırılmasında kullanılan çeşitli metotlar vardır. Güvenilir sonuçların elde edilmesinde infeksiyon etkeni olan mikroorganizmalar için duyarlılık testlerinin hassasiyeti halen birçok araştırıcı tarafından araştırılmakta ve farklı yöntemlerin kıyaslamaları yapılmaktadır. Stafilokoklar, hem hastane infeksiyonu hem de toplumsal kaynaklı birçok infeksiyona neden olan önemli bakterilerdir. Hem kolonize hem de infekte hastalarda Staphylococcus aureus’ un duyarlılık aralığı tanıda ve tedavide oldukça önemlidir. Naşide Halil Gelendost ilköğretim okulundaki 7-12 yaşlar arasındaki öğrencilerden izole edilen S.aureus izolatlarına karşı trimetoprim-sulfametoksazol, penisilin, tetrasiklin, vankomisin ve eritromisin ajanlarının in-vitro aktivitesi mikrodilüsyon ve disk difüzyon yöntemleriyle araştırılmıştır. Bu iki yöntem sonuçları, NCCLS kriterlerine göre kıyaslandığında 65 izolat arasında % 97.5 oranında uyum görülmüştür.

🔖

Cite This Article

ÖZÇELİK, B., KAYNAK, F., ABBASOĞLU, U. (2004). Stafilokoklarda sıvı mikrodilüsyon ve disk difüzyon yöntemlerinin kıyaslanmasıyla duyarlılık testlerinin değerlendirilmesi. Turkish Journal of Pharmaceutical Sciences, Cilt: 1 - Sayı: 2, 77–86. https://ajindex.com/articles/403867

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Gesu, G.P., Marchetti, F., Piccoli, L., Cavallero, A., "Levofloxacin and ciprofloxacin in-vitro activities against 4003 clinical bacterial isolates collected in 24 Italian laboratories" Antimicrob Agents.Chemother., 47(2), 816-19, 2003
  2. McGuire, M.D., Conard, R.S., "Morphological and Biochemical alteration in Staphylococcus epidermidis stepwise adapted to vancomycine resistance" Proc.Okla AcadSci., 80:1-6,2000
  3. Goettsch, W., Bronzwaer, S., Neeling, A., Wale, M., Aubry-Damon, H., Olsson-Liljeqquist, B., Sprenger, M., Degener, J., "Standardization of Streptococcus pneumonia and Staphylococcus aureus susceptibility data within EARSS" ClinMicrobi-oUnfect., 6, 59-63, 2000
  4. Woods, W., Ramotar, K., Lem, P., Toye, B., "Oxacillin susceptibility testing of co-agulase-negative Staphylococci using the disk diffusion method and the Vitek GPS-105 card" Diagnostic MicrobiolInfect,Dis., 42, 291-4, 2002
  5. Ambaye, A., Kohner, P.C., Wollan, P.C., Roberts, K.L., Roberts, G.D., Cocke-rill, F.R., "Comparison of Agar Dilution, Broth Microdilution, Disk diffusion, E-Test, and BACTEC Radiometric methods for Antimicrobial susceptibility testing of clinical isolates of Nocardia asteroids complex" J.Clin.Microbiol., 847-52,1997
  6. Fuchs, P.C., Barry, A.L., Brown, S.D., "In-vitro activities of clinafloxacin againstcontemporary clinical bacterial isolates from 10 North American centers" J Antimicrob.Chemother., 42,5,1998
  7. Jawetz, Melnick & Adelberg's- Medical Microbiology, Eds: F. G.Brooks, J.S., Butel,L.N., Ornston, pp. 94-199, Pentice Hall, London, 1991
  8. Sham D.F., Washington J.A. Antibacterial Susceptibility Tests, Dilution Methods.In: Balowes A, Hausler WJ, Hermann KL, Shadomy HD, editors. Manuel of Clinical Microbiology. Washington DC: Am.Soc.Microbiol; 1991 pp 1105
  9. National Committee for Clinical Laboratory Standarts "Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically-Third Edition" Approved standard. NCCLS document M100-S12, NCCLS, 940 West Valley Road,Wayne, Pennsylvania 19087, 2002
  10. Mendes, R.E., Reis, A.O., Gales, A.C., Jones, R.N., Sader, H.S., "Ability of LatinAmerica Laboratories to detect Antimicrobial resistance patterns: Experience of theSentry antimicrobial surveillance program (1997-2000)" The Brazilian J.Infect.Dis.,7(5), 282-9, 2003
  11. Vanteen, A., Buiting, A., KSuytmans, J., In vitro activity of linezolid in methicil-lin-resistant and methicillin-susceptible Staphylococcus aureus in the Netherl