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Journals / Turkish Journal of Pharmaceutical Sciences / 2016 / Cilt: 13 - Sayı: 2

Inula viscosa (L.) Aiton Çiçeklerinde YPSK ve YPİTK Yöntemleri ile Hispidulin Analizi

Determination of Hispidulin in the flowers of Inula viscosa (L.) Aiton Using HPLC and HPTLC Methods

ALPER GÖKBULUT
Pages
159–166
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results reveal that I. viscosa flowers contain serious amount (0.151± 0.007 g/100 g dry weight) of hispidulin.

📌 3 Key Takeaways

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Objective & Hypothesis:The flavone hispidulin (4´,5,7-trihydroxy-6-methoxyflavone) in the flowers of Inula viscosa (L.) Aiton was quantified by using High Performance Liquid Chromatography (HPLC), and also High Performance Thin-Layer Chromatography (HPTLC) fingerprint of the methanolic I. viscosa flower extract was presented.
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Key Findings & Data:Both methods provided a rapid and easy approach for determination of the biomarker hispidulin.
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Conclusion & Impact:The results reveal that I. viscosa flowers contain serious amount (0.151± 0.007 g/100 g dry weight) of hispidulin.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The flavone hispidulin (4´,5,7-trihydroxy-6-methoxyflavone) in the flowers of Inula viscosa (L.) Aiton was quantified by using High Performance Liquid Chromatography (HPLC), and also High Performance Thin-Layer Chromatography (HPTLC) fingerprint of the methanolic I. viscosa flower extract was presented. Both methods provided a rapid and easy approach for determination of the biomarker hispidulin. In the present study HPTLC and HPLC profiles of Inula viscosa flowers were presented to detect and quantify the small flavonoid hispidulin. The results reveal that I. viscosa flowers contain serious amount (0.151± 0.007 g/100 g dry weight) of hispidulin.

Özet

Inula viscosa (L.) Aiton Çiçeklerinde YPSK ve YPİTK Yöntemleri ile Hispidulin Analizi Inula viscosa (L.) Aiton çiçeklerinde bir flavon bileşiği olan hispidulin (4´,5,7-trihidroksi-6- metoksiflavon) miktarı Yüksek Performanslı Sıvı Kromatografisi (YPSK) ile tayin edilmiş, ayrıca I. viscosa çiçeklerinin metanolik ekstresinin Yüksek Performanslı İnce Tabaka Kromatografisi (YPİTK) parmakizi kromatogramı da verilmiştir. Her iki yöntem de biyo-işaretleyici bileşen olan hispidulinin belirlenmesi için hızlı ve kolay yaklaşımlar sağlamaktadır. Bu çalışmada küçük bir flavonoit bileşiği olan hispidulinin teşhis ve miktar tayinini gerçekleştirmek amacıyla I. viscosa çiçeklerinin YPİTK ve YPSK profilleri ortaya konmuştur. Sonuçlar, I. viscosa çiçeklerinin önemli ölçüde (0.151±0.007 g/100 g kuru ağırlık) hispidulin içerdiğini göstermektedir.

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Cite This Article

GÖKBULUT, A. (2016). Inula viscosa (L.) Aiton Çiçeklerinde YPSK ve YPİTK Yöntemleri ile Hispidulin Analizi. Turkish Journal of Pharmaceutical Sciences, Cilt: 13 - Sayı: 2, 159–166. https://ajindex.com/articles/403720

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