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Journals / Sağlık Bilimleri Dergisi / 2017 / Cilt: 26 - Sayı: 1

Determination of Total Phenolic and Flavonoid Contents and Antioxidant Effects of Eryngium Billarderi Delar. Extracts

ERYNGIUM BILLARDERI DELAR. EKSTRELERİNİN TOPLAM FENOLİK VE FLAVONOİT MADDE KOMPOZİSYONLARININ VE ANTİOKSİDAN ETKİLERİNİN BELİRLENMESİ

Leyla PAŞAYEVA
Esra KÖNGÜL
Gökçe Şeker KARATOPRAK
Osman TUGAY
Pages
18–23
Publication Date
2017-03-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The butanol fraction of methanol extract characterized by the presence of steroidal and triterpenic saponins, ethyl acetate fraction exhibited the highest antiradical activity which was found rich in flavonoids and phenolics.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, we investigated the composition of the extract prepared from aerial parts of Eryngium billardieri Delar. (Apiaceae family) with 70% methanol and fractions of this extract with TLC analysis, saponin composition with saponin tests, DPPH● and ABTS●+ radical- scavenging activity, the total phenolic content with AlCl3 colorimetric assay and the total flavonoid content with Folin−Ciocalteu assay.
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Key Findings & Data:In this study, we investigated the composition of the extract prepared from aerial parts of Eryngium billardieri Delar. (Apiaceae family) with 70% methanol and fractions of this extract with TLC analysis, saponin composition with saponin tests, DPPH● and ABTS●+ radical- scavenging activity, the total phenolic content with AlCl3 colorimetric assay and the total flavonoid content with Folin−Ciocalteu assay.
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Conclusion & Impact:The butanol fraction of methanol extract characterized by the presence of steroidal and triterpenic saponins, ethyl acetate fraction exhibited the highest antiradical activity which was found rich in flavonoids and phenolics.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Eryngium billardieri · Apiaceae · Antiradikal aktivite

Abstract

In this study, we investigated the composition of the extract prepared from aerial parts of Eryngium billardieri Delar. (Apiaceae family) with 70% methanol and fractions of this extract with TLC analysis, saponin composition with saponin tests, DPPH● and ABTS●+ radical- scavenging activity, the total phenolic content with AlCl3 colorimetric assay and the total flavonoid content with Folin−Ciocalteu assay. The butanol fraction of methanol extract characterized by the presence of steroidal and triterpenic saponins, ethyl acetate fraction exhibited the highest antiradical activity which was found rich in flavonoids and phenolics.

Özet

Bu çalışma kapsamında Apiaceae familyasına dahil Eryngium billardieri Delar. 'ın toprak üstü kısımlarından hazırlanan %70 metanol ekstresinin ve bu ekstreden elde edilen fraksiyonların İnce Tabaka Kromatografisi (İTK) ile analizleri, saponin teşhis reaksiyonları ile saponin içerikleri, DPPH● ve ABTS●+radikallerini süpürücü aktiviteleri, Folin- Ciocalteu yöntemi ile toplam fenolik, AlCl3 kolorimetrik yöntemi ile toplam flavonoit madde miktarı incelenmiştir. Metanol ekstresinden elde edilen butanol fraksiyonunun steroidal ve triterpenik saponin içerdiği aydınlatılırken, etil asetat fraksiyonunun toplam flavonoit ve fenol bakımından zengin olduğu ve antiradikal aktivite deneylerinde de güçlü etki gösterdiği belirlenmiştir.

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Cite This Article

PAŞAYEVA, L., KÖNGÜL, E., KARATOPRAK, G. Ş., TUGAY, O. (2017). Determination of Total Phenolic and Flavonoid Contents and Antioxidant Effects of Eryngium Billarderi Delar. Extracts. Sağlık Bilimleri Dergisi, Cilt: 26 - Sayı: 1, 18–23. https://ajindex.com/articles/403067

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