SavedAdd your journal

Journals / Atatürk Üniversitesi Ziraat Fakültesi Dergisi / 2019 / Cilt: 50 - Sayı: 3

Investigation of Biological Control Possibility of Anthracnose Disease Agent, Colletotrichum gloeosporioides, on Orange

Portakalda Antraknoz Hastalığı Etmeni Colletotrichum gloeosporioides’in Biyolojik Mücadele İmkanlarının Araştırılması

Nasibe TEKİNER
Elif TOZLU
Recep KOTAN
Pages
282–291
Publication Date
2019-09-22
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result of the application, it was determined that all microbial antagonists prevented the development of pathogen fungus in vitro and P. fluorescens MF 3 isolate (69.05%) and T. harzianum ET 4 isolates (82.50%) were the most effective isolates.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The negative effect of fungicides on human and environmental health, resistance to fungicides and the problem of residue in agricultural products are getting increase the importance of biological control one of the alternative methods for chemical control.
📊
Key Findings & Data:Nine bioagent bacterial isolates [Bacillus megaterium (TV 3D), Bacillus subtilis (TV 6F, TV 17C, CP 1), Bacillus cereus (TV 85D), Paenibacillus polymxa (TV 12E), Pantoea agglomerans (RK 79, RK 92), Pseudomonas fluorescens (MF 3)] and three bioagent fungi isolates [Trichoderma harzianum (ET 4, ET 14, NT 1)] were used in this study the investigation of biological control of anthracnose disease (Colletotrichum gloesporoides) common in vegetables and fruits in in vitro.
💡
Conclusion & Impact:As a result of the application, it was determined that all microbial antagonists prevented the development of pathogen fungus in vitro and P. fluorescens MF 3 isolate (69.05%) and T. harzianum ET 4 isolates (82.50%) were the most effective isolates.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Portakal · Colletotrichum gloesporoides · Pseudomonas fluorescens

Abstract

The negative effect of fungicides on human and environmental health, resistance to fungicides and the problem of residue in agricultural products are getting increase the importance of biological control one of the alternative methods for chemical control. Nine bioagent bacterial isolates [Bacillus megaterium (TV 3D), Bacillus subtilis (TV 6F, TV 17C, CP 1), Bacillus cereus (TV 85D), Paenibacillus polymxa (TV 12E), Pantoea agglomerans (RK 79, RK 92), Pseudomonas fluorescens (MF 3)] and three bioagent fungi isolates [Trichoderma harzianum (ET 4, ET 14, NT 1)] were used in this study the investigation of biological control of anthracnose disease (Colletotrichum gloesporoides) common in vegetables and fruits in in vitro. As a result of the application, it was determined that all microbial antagonists prevented the development of pathogen fungus in vitro and P. fluorescens MF 3 isolate (69.05%) and T. harzianum ET 4 isolates (82.50%) were the most effective isolates.

Özet

Fungisitlerin insan ve çevre sağlığını olumsuz etkilemesi, hastalık etmenlerinin fungisitlere karşı direnç oluşturması ve tarımsal ürünlerdeki kalıntı sorunu kimyasal mücadeleye alternatif yöntemlerden olan biyolojik mücadelenin önemini bir kat daha artırmıştır. Sebze ve meyvelerde yaygın olarak görülen antraknoz hastalığı etmeninin (Colletotrichum gloesporoides) in vitro koşullarda mikrobiyal antagonistlerle mücadele edilebilirliğinin araştırıldığı bu çalışmada toplam 9 adet biyoajan bakteri izolatı [Bacillus megaterium (TV 3D), Bacillus subtilis (TV 6F, TV 17C, CP 1), Bacillus cereus (TV 85D), Paenibacillus polymxa (TV 12E), Pantoea agglomerans (RK 79, RK 92), Pseudomonas fluorescens (MF 3)] ve 3 adet biyoajan fungus izolatı [Trichoderma harzianum (ET 4, ET 14, NT 1)] kullanılmıştır. Biyoajan fungusların patojene karşı etkililiği petri kaplarına karşılıklı konularak, bakterilerin ise merkeze patojen konulup etrafına bakteri çizilerek yapılmıştır. Uygulama sonucunda tüm mikrobiyal antagonistlerin in vitro şartlarda patojen fungusun gelişimini engellediği ve P. fluorescens’in MF 3 izolatının (%69,05) ve T. harzianum’un ET 4 izolatının (%82,50) en etkili izolatlar olduğu belirlenmiştir.

🔖

Cite This Article

TEKİNER, N., TOZLU, E., KOTAN, R. (2019). Investigation of Biological Control Possibility of Anthracnose Disease Agent, Colletotrichum gloeosporioides, on Orange. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, Cilt: 50 - Sayı: 3, 282–291. https://ajindex.com/articles/36885

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Akgün, C., 2006. Turunçgiller Sektör Profili. Dış Ticaret Servisi Uygulama Şubesi, Türkiye. FAO (2017) Food and Agriculture Organisation of the United Nations, FAOSTAT Agriculture Data. Rome, Italy:. (http://faostat.fao.org) (Erişim tarihi 10.03.2019). TÜİK, 2017. Türkiye İstatistik Kurumu (www.tuik.gov.tr) (Erişim tarihi 10.03.2019).
  2. Benli, M., 2003. Hasat sonrası fungal hastalıklarla kimyasal ve biyolojik mücadele. Orlab On Line Mikrobiyoloji Dergisi, Yıl: 01(08): 1-25.
  3. Singh, D., Sharma, R.R., 2007. Postharvest diseases of fruit and vegetables and their management. In: Prasad, D. (Ed.), Sustainable Pest Management. Data Publishing House, New Delhi, India. Karaçalı, İ., 1993. Bahçe ürünlerinin muhafaza ve pazarlanması. Ege üniversitesi yayınları, İzmir,494p. Aloui, H., Khwaldia, K., Licciardello, F., Mazzaglia, A., Muratore, G, Hamdi, M., 2014. Efficacy of the combined application of chitosan and locust bean gum with different citrus essential oils to control postharvest spoilage caused by Aspergillus flavus in dates. Int. J. Food Microbio., 170: 21-28.
  4. Zhang, J.X., Timmer, L.W., 2007. Preharvest application of fungicides for postharvest disease control on early season tangerine hybrids in Florida. Crop. Prot., 26: 886-893.
  5. Mahoney, M.J., Tattar, T.A, 1980. Identification, etiology and control of Euonymus fortunei anthracnose caused by Colletotrichum gloeosporioides. Plant Dis., 64: 854-856.
  6. Dubey, N., Srivastava, B., Kumar, A., 2008. Current status of plant products as botanical pesticides in storage pest management. Journal Biopesticide, 1(2): 182-186.
  7. Ongena, M., Jacques, P., 2008. Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends in Microbiolilogy, 16(3): 115-125.
  8. Talibi, I., Boubaker, H., Boudyach, E.H., Aoumar, A.A.B., 2014. Alternativa methods for the control of postharvest citrus diseases. J. Appl. Microbiol., 117: 1-17.
  9. Than, P.P, Jeewon, R., Hyde, K.D., Pongsupasamit, S., Mongkolporn, O., Taylor, P.W.J. Characterization and pathogenicity of Colletotrichum species associated with anthracnose disease on chilli (Capsicum spp.) in Thailand. Plant Pathol., 57: 562-572.
  10. Yamamoto, S., Shiraishi, S., Suzuki, S., 2014. Are cyclic lipopeptides produced by Bacillus amyloliquefaciens S13-3 responsible for the plant defence response in strawberry against Colletotrichum gloeosporioides. Letters in Applied Microbiology, 60: 379-386.
  11. Zhou, Y., Zhang, L., Zeng K., 2016. Efficacy of Pichia membranaefaciens combined with chitosan again