Journals / Eurasian Journal of Agricultural Research / 2017 / Cilt: 1 - Sayı: 2

Molecular Identification, Virulens and Genetic Diversity of Fusarium species on Wheat

Pages
13–22
Publication Date
2017-11-30
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Using phylogenetic analyses based on the internal transcribed spacer sequence data, it was examined by genetic variations between species of Fusarium.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Surveys were conducted in Konya, Ankara, Eskisehir, Kayseri, Nevsehir, Aksaray, Yozgat, Kirsehir and Kirikkale province in 2011 and 2012 growing seasons and 1256 diseased wheat samples were collected from 2770 wheat fields.
📊
Key Findings & Data:As a result of isolation from this samples 668 Fusarium isolates were obtained belonging to 18 different species.
💡
Conclusion & Impact:Using phylogenetic analyses based on the internal transcribed spacer sequence data, it was examined by genetic variations between species of Fusarium.
🔬Design:Survey & Empirical Study
📍Scope:2011 Verileri
⚙️Variables:Fusarium spp. · Triticum aestivum · molecular

Abstract

Surveys were conducted in Konya, Ankara, Eskisehir, Kayseri, Nevsehir, Aksaray, Yozgat, Kirsehir and Kirikkale province in 2011 and 2012 growing seasons and 1256 diseased wheat samples were collected from 2770 wheat fields. As a result of isolation from this samples 668 Fusarium isolates were obtained belonging to 18 different species. In consequence of DNA sequence analysis, obtained isolates were determined Fusarium acuminatum, F. avenaceum, F. brachygibbosum, F. cerealis, F. chlamydosporum, F. culmorum, F. equiseti, F. graminearum, F. incarnatum, F. lacertarum, F. longipes, F. nivale (Microdochium nivale), F. oxysporum, F. proliferatum, F. redolens, F. solani, F. sporotrichioides, F. tricinctum. Pathogenicity tests performed and it was determined that the most virulent group were respectively, 86,72-96%, 84,67-94% ve 80,67-94,67% with diseases severity F. culmorum, F. tricinctum and F. nivale. The most commonly isolated species was F. nivale. Using phylogenetic analyses based on the internal transcribed spacer sequence data, it was examined by genetic variations between species of Fusarium.

🔖

Cite This Article

ÜNAL, F., DOLAR, F. S., TEKİNER, N., YEĞİN, N. Z. (2017). Molecular Identification, Virulens and Genetic Diversity of Fusarium species on Wheat. Eurasian Journal of Agricultural Research, Cilt: 1 - Sayı: 2, 13–22. https://ajindex.com/articles/755919

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Appel DJ, Gordon TR.1996. Relationships among pathogenic and non-pathogenic isolates of Fusarium oxysporum based on the partial sequence of the intergenic spacer region of the ribosomal DNA. Molecular Plant Microbe Interactions, 9, 125-38.
  2. Aroca, A., Raposo, R. 2007. PCR-based stragety to detect and identify species of Phaeoacremonium causing grapevine diseases. Applied and Environmental Microbiology, 73, 2911-2918.
  3. Bentley, A. R., Tunali, B., Nicol, J., Burgess, L. W., and Summerell, B. A. 2006. A survey of Fusarium species associated with wheat stem bases in northern Turkey. Sydowia 58:163-177.
  4. Bottalico, A., & Perrone, G. 2002. Toxigenic Fusarium species and mycotoxins associated with head blight in small-grain cereals in Europe. European Journal of Plant Pathology, 108, 611–624.
  5. Burgess, L. W., Backhouse, D., Summerell, B.A., and Swan, L. J. 2001. Crown rot of wheat. Pages 271-295 in: Fusarium – Paul E. Nelson Memorial Symposium. B. A. Summerell, J. F. Leslie, D. Backhouse, W. L. Bryden, and L. W. Burgess, eds. American Phytopathological Society. Cobos, R., Martin, M.T. 2008. Molecular characterisation of Phaeomoniella chlamydospora isolated from grapevines in Castilla y León (Spain). Phytopathol. Mediterr., 47, 20–27.
  6. Demirci, E., and Dane, E. 2003. Identification and pathogencity of Fusarium spp. from stem bases of winter wheat in Erzurum, Turkey. Phytoparasitica 31:170-173.
  7. Fernandez, M. R., and Chen, Y. 2005. Pathogenicity of Fusarium species on different plant parts of spring wheat under controlled conditions. Plant Dis. 89:164-169.
  8. Ichielevich- Auster M., Sneh, B., Koltin Y., Barash I.,1985. Suppression of Damping-Off Caused by Rhizoctonia Species by a Nonpathogenic Isolate of R. solani. Phytopathology 75:1080-1084.
  9. Karman, M. 1971. Bitki Koruma Araştırmalarında Genel Bilgiler, Denemelerin Kuruluşu ve Değerlendirme Esasları, Bornova-İzmir, 279s. Lemmens, M., Bűrstmayr, H., Krska, R., Schumacher, R., Grausgruber, H., & Ruckenbauer, P. 2004. The effect of inoculation treatment and long-term application of moisture on Fusarium head blight symptoms and deoxynivalenol contamination in wheat grains. European Journal of Plant Pathology, 110, 229–308. Leslie JF and Summerell BA 2006 The Fusarium Laboratory manual. (Blackwell Publishing: Iowa, USA). Mishra PK, Fox RT, Culham A. 2003. Development of a PCR-based assay for rapid and reliable identification of pathogenic Fusaria. FEMS Microbiol Lett,218(2): 329-32.
  10. Nicholson P, Simpson DR, Weston G, Rezanoor