Serum adenosine deaminase activity in smokers and users of smokeless tobacco

Objective & Hypothesis:Purpose: Adenosine deaminase (ADA) is essential for the differentiation of lymphoid cells, particularly T cells, and plays a role in the maturation of mono-cytes to macrophages.

📊

Key Findings & Data:Material and Methods: We compared the serum ADA levels in smokers (n=39), in users of smokeless tobacco (n=47) and in healthy control subjects (n=33).

💡

Conclusion & Impact:Conclusion: Both tobacco and smokeless tobacco increase the serum ADA activity.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Sigara içme · Adenozin deaminaz · Tütün

Abstract

Purpose: Adenosine deaminase (ADA) is essential for the differentiation of lymphoid cells, particularly T cells, and plays a role in the maturation of mono-cytes to macrophages. ADA, an important enzyme in purine and DNA metabolism is considered to be an indicator of cell-mediated immunity. Tobacco causes DNA damage because of its toxic contents. The aim of this study was to evaluate the diagnostic value of serum ADA activity in smokers and Maraş powder users (a variety of smokeless tobacco). Material and Methods: We compared the serum ADA levels in smokers (n=39), in users of smokeless tobacco (n=47) and in healthy control subjects (n=33). Results: The ADA activities were 23.03±6.26 U/L in smokers, 25.18±12.25 U/L in smokeless tobacco group, and 17.20±4.50 U/L in control subjects. The results of smoker and smokeless tobacco group were statistically significant higher than control group (p=0.017, p=0.001 respectively). There was no statistically significant differences between the smokers and smokeless tobacco group (p>0.05). The serum ADA activities did not correlate with the duration of substance use. Conclusion: Both tobacco and smokeless tobacco increase the serum ADA activity.

Özet

Amaç: Adenosin Deaminaz (ADA), lenfoid hücrelerin farklılaşmasında, özellikle T lenfositler ve monositlerden makrofaj gelişiminde önemli rol oynarlar. ADA hücresel bağışıklık sisteminde, pürin ve DNA metabolizmasında önemli bir enzimdir. Sigara içerdiği birçok toxic ajandan dolayı DNA hasarı yapmaktadır. Bu çalışmada, sigara ve Maraş otu (bir çeşit dumansız tütün kullanımı) kullananlarda serum ADA aktivitesi değerlendirildi. Gereç ve Yöntem: Sigara içen (n=39), dumansız tütün kullanan (n=47) ve sağlıklı kontrol (n=33) grubunda serum ADA aktiviteleri karşılaştırıldı. Bulgular: Serum ADA aktivitesi sigara içen grupta 23.03±6.26 U/L, dumansız tütün kullanan grupta 25.18±12.25 U/L, ve kontrol grubunda 17.20±4.50 U/L idi. Sigara içen ve dumansız tütün kullanan grupta ADA aktiviteleri kontrol grubuna oranla istatistiksel olarak daha yüksek bulundu ( sırasıyla p=0.017, p=0.001). Serum ADA aktivitesi, sigara içen grup ile dumansız tütün kullananlar arasındaki fark istatistiksel olarak anlamsızdı (p>0.05). Sigara ve dumansız tütün kullanım süresi ile serum ADA aktivitesi arasında korelasyon yoktu (p>0.05). Sonuç: Sigara içimi ve dumansız tütün kullanımı serum ÂDA aktivitesini arttırmaktadır.

Keywords

Sigara içme Adenozin deaminaz Tütün dumansız Smoking Adenosine Deaminase Tobacco Smokeless

🔖

Cite This Article

KÖKSAL, N., TOLUN, F. İ., KILINÇ, M. (2004). Serum adenosine deaminase activity in smokers and users of smokeless tobacco. Tıp Araştırmaları Dergisi, Cilt: 2 - Sayı: 3, 7–11. https://ajindex.com/articles/361759

📄 Full Text PDF Viewer

Open in New Tab ↗

↗New Tab

📄

PDF görüntüleyici hazırlanıyor...

References (16)

Camici M, Tozzi MG, Allegrini S, et al. Purine salvage enzyme activities in normal and neoplastic human tissues. Cancer Biochem Biophys 1990
Cristalli G, Costanzi S, Lambertucci C, et al. Adenosine Deaminase: Functional implications and different classes of inhibitors. Med Res Rev. 2001
21(2):105-128. 3.Akyol O, Gökbulut I, Köksal N, et al. The activities of purine catabolizing enzymes in plasma and bronchial washing fluid in patients with lung cancer and pneumonia, Clinical Biochemistry 2001
Yuksel H, Akoglu TF. Serum and synovial fluid adenosine deaminase activity in patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis. Ann Rheum Dis 1988
47(6):492-495. 5.Bota A, Gella FJ, Canalias F. Optimization of adenosine deaminase assay by response surface methodology. Clinica Chimica Acta 2000
Molinos L, Fernandez R, Dominguez MJ, et al. Adenosine deaminase activity in the aetiological diagnosis of community-acquired pneumonia. Scan J Infect Dis 1997
Ocana I, Martinez-Vazquez JM, Ribera E, et al. Adenosine deaminase activity in the diagnosis of lymphocytic pleural effusions of tuberculosis, neoplastic and lymphomatous origin. Tubercle 1986
Ocana I, Martinez-Vazquez JM, Segura RM, et al. Adenosine deaminase in pleural fluids. Test for diagnosis of tuberculous pleural effusion. Chest 1983
Galanti B, Nardiello S, Russo M, Fiorentino F. Increased lymphocyte adenosine deaminase in typhoid fever. Scan J Infect Dis 1981
Dikensoy O, Namiduru M, Hocaoglu S, et al. Increased pleural fluid adenosine deaminase in brucel- losis is difficult to differentiate from tuberculosis. Respiration 2002
69(6):556-559. 11.Öztuna F. Sigaranın hücresel etkileri. Akciğer Arşivi 2004
Erenmemişoğlu A, Tekol Y, Kartal M, Kurucu S. Yurdumuzda dumansız bir tütün kullanımı: Maraş otu. Doğa-Turk J of Med Sci 1992
Erenmemisoglu A. Re: Turkish smokeless tobacco "Maras Powder" Prev Med 1999
Kilinc M, Okur E, Kurutas EB, et al. The effects of Maras powder (smokeless tobacco) on oxidative stress in users. Cell Biochem Funct 2004
22(4):233-236. 15.Pfeifer GP, Denissenko MF, Olivier M, et al. Tobacco smoke carcinogens, DNA damage and p53 mutations in smoking-associated cancers. Oncogene 2002
21(48):7435-7451. 16.Ohnishi S, Murata M, Kawanishi S. Oxidative DNA damage induced by a metabolite of 2-naphthylamine, a smoking related bladder carcinogen.