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Journals / Ziraat Fakültesi Dergisi / 2015 / Cilt: 10 - Sayı: 2

Genetic Divergence in Some Barley (Hordeum vulgare L.) Genotypes by RAPD and ISSR Analyses

Bazı Arpa (Hordeum vulgare L.) Genotiplerinin Genetik Farklılığının RAPD ve ISSR Analizleri ile Belirlenmesi

MURAT OLGUN
ZEKİYE BUDAK BAŞÇİFTÇİ
N.Gözde AYTER
Metin TURAN
ONUR KOYUNCU
MURAT ARDIÇ
Güleray AGAR
ENGİN TAKIL
Pages
102–109
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results showed that average polymorphic rate was 86.5%.

📌 3 Key Takeaways

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Objective & Hypothesis:The objective of this study was to determine genetic distance between barley genotypes based on RAPD and ISSR analyses.
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Key Findings & Data:The number of amplified bands of genotypes in the primers ranged 4-11 in RAPD and 7-11 in ISSR.
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Conclusion & Impact:Results showed that average polymorphic rate was 86.5%.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Arpa · genetik ayrışma · DNA ektrasyonu

Abstract

The objective of this study was to determine genetic distance between barley genotypes based on RAPD and ISSR analyses. The number of amplified bands of genotypes in the primers ranged 4-11 in RAPD and 7-11 in ISSR. The most polymorphic bands in primer/primer combinations were obtained from OPA-04, OPA-13 and OPH-17 in RAPD, and UBC-808, UBC-820 and UBC-872 in ISSR. Results showed that average polymorphic rate was 86.5%. Performances of cultivars in groups asist possibility in creating genetic variability in barley cultivar development. RAPD and ISSR methods are useful for evaluation of genetic diversity and could be safely used to determine the genetic relationships in barley genotypes.

Özet

The objective of this study was to determine genetic distance between barley genotypes based on RAPD and ISSR analyses. The number of amplified bands of genotypes in the primers ranged 4-11 in RAPD and 7-11 in ISSR. The most polymorphic bands in primer/primer combinations were obtained from OPA-04, OPA-13 and OPH-17 in RAPD, and UBC-808, UBC-820 and UBC-872 in ISSR. Results showed that average polymorphic rate was 86.5%. Performances of cultivars in groups asist possibility in creating genetic variability in barley cultivar development. RAPD and ISSR methods are useful for evaluation of genetic diversity and could be safely used to determine the genetic relationships in barley genotypes.

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Cite This Article

OLGUN, M., BAŞÇİFTÇİ, Z. B., AYTER, N., TURAN, M., KOYUNCU, O., ARDIÇ, M., AGAR, G., TAKIL, E. (2015). Genetic Divergence in Some Barley (Hordeum vulgare L.) Genotypes by RAPD and ISSR Analyses. Ziraat Fakültesi Dergisi, Cilt: 10 - Sayı: 2, 102–109. https://ajindex.com/articles/359962

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