Dergiler / Ziraat Fakültesi Dergisi / 2015 / Cilt: 10 - Sayı: 2

Bazı Arpa (Hordeum vulgare L.) Genotiplerinin Genetik Farklılığının RAPD ve ISSR Analizleri ile Belirlenmesi

Genetic Divergence in Some Barley (Hordeum vulgare L.) Genotypes by RAPD and ISSR Analyses

Sayfa
102–109
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

RAPD and ISSR methods are useful for evaluation of genetic diversity and could be safely used to determine the genetic relationships in barley genotypes.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:The objective of this study was to determine genetic distance between barley genotypes based on RAPD and ISSR analyses.
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Temel Bulgular & Veri:The number of amplified bands of genotypes in the primers ranged 4-11 in RAPD and 7-11 in ISSR.
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Sonuç & Katkı:RAPD and ISSR methods are useful for evaluation of genetic diversity and could be safely used to determine the genetic relationships in barley genotypes.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Arpa · genetik ayrışma · DNA ektrasyonu

Özet

The objective of this study was to determine genetic distance between barley genotypes based on RAPD and ISSR analyses. The number of amplified bands of genotypes in the primers ranged 4-11 in RAPD and 7-11 in ISSR. The most polymorphic bands in primer/primer combinations were obtained from OPA-04, OPA-13 and OPH-17 in RAPD, and UBC-808, UBC-820 and UBC-872 in ISSR. Results showed that average polymorphic rate was 86.5%. Performances of cultivars in groups asist possibility in creating genetic variability in barley cultivar development. RAPD and ISSR methods are useful for evaluation of genetic diversity and could be safely used to determine the genetic relationships in barley genotypes.

Abstract

The objective of this study was to determine genetic distance between barley genotypes based on RAPD and ISSR analyses. The number of amplified bands of genotypes in the primers ranged 4-11 in RAPD and 7-11 in ISSR. The most polymorphic bands in primer/primer combinations were obtained from OPA-04, OPA-13 and OPH-17 in RAPD, and UBC-808, UBC-820 and UBC-872 in ISSR. Results showed that average polymorphic rate was 86.5%. Performances of cultivars in groups asist possibility in creating genetic variability in barley cultivar development. RAPD and ISSR methods are useful for evaluation of genetic diversity and could be safely used to determine the genetic relationships in barley genotypes.

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Bu Makaleye Atıf Yap

OLGUN, M., BAŞÇİFTÇİ, Z. B., AYTER, N., TURAN, M., KOYUNCU, O., ARDIÇ, M., AGAR, G., TAKIL, E. (2015). Bazı Arpa (Hordeum vulgare L.) Genotiplerinin Genetik Farklılığının RAPD ve ISSR Analizleri ile Belirlenmesi. Ziraat Fakültesi Dergisi, Cilt: 10 - Sayı: 2, 102–109. https://ajindex.com/articles/359962

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