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Journals / Records of Natural Products / 2018 / Cilt: 12 - Sayı: 6

The Protective Effect and MoA of Panax notoginseng Saponins Combined with Aminoguanidine on Kidney in Diabetic Nephropathy Rats

Guangfu LV
Zhikai QİU
Hongbo YE
Chao MA
Shizhuo CHANG
Zhe LİN
Pages
602–610
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The mechanism of PNS combined with AG in the treatment of DN may be closely related to the reduction of urinary protein, anti-inflammatory response, and inhibition of the expression of TGF-β1.

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Objective & Hypothesis:This study was conducted to explore the protecting effect and treatment mechanism of Panax notoginseng saponins (PNS) in combination with aminoguanidine (AG) on kidney functions in diabetic nephropathy (DN) rats.
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Key Findings & Data:Rats were divided into 5 groups (A, P, P+A, D and N) and diabetes was induced by streptozotocin (STZ).
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Conclusion & Impact:The mechanism of PNS combined with AG in the treatment of DN may be closely related to the reduction of urinary protein, anti-inflammatory response, and inhibition of the expression of TGF-β1.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Özet

This study was conducted to explore the protecting effect and treatment mechanism of Panax notoginseng saponins (PNS) in combination with aminoguanidine (AG) on kidney functions in diabetic nephropathy (DN) rats. Rats were divided into 5 groups (A, P, P+A, D and N) and diabetes was induced by streptozotocin (STZ). After 8 weeks, all rats were sacrificed and the renal functions as well as 24 hrs urinary proteins were examined quantitatively. Periodic acid-Schiff stain (PAS) was used to observe the pathological changes of renal tissue. Immune-nephelometry, immunoradiometric assay, Immunohistochemical and real-time fluorescence were used for blood C-reactive protein, tumor necrosis factor-α (TNF-α), surface specific marker antigen (ED-1) and determination of transforming growth factor- β1 (TGF-β1) in kidney tissue. There was a significant reduction in renal pathological changes of rats in group P, A, and P+A when compared with group D, while the changes were most obvious in group P+A. Blood glucose, glomerular volume (V), serum creatinine, 24 hrs urinary protein, blood CRP and TNF- in groups P, A and P+A were significantly (P<0.05) lower than group D with lowest in group P+A. Immunohistochemical staining showed, only a few ED-1 positive staining (macrophages) in group N glomeruli and the renal tubules. The staining of ED-1 group D was higher while in groups P, A and P+A was significantly reduced. There was a basic quantity of TGF-β1 mRNA expression in group N rats while the expression of TGF-β1 mRNA in group D was significantly increased when compared with control group. In group P, A and P+A, the expression level of TGF-β1 mRNA was significantly (P<0.05) decreased. The protective effect of combined use of these two PNS and AG on the kidney is superior to the single drug treatment. The mechanism of PNS combined with AG in the treatment of DN may be closely related to the reduction of urinary protein, anti-inflammatory response, and inhibition of the expression of TGF-β1.

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Cite This Article

LV, G., QİU, Z., YE, H., MA, C., CHANG, S., LİN, Z. (2018). The Protective Effect and MoA of Panax notoginseng Saponins Combined with Aminoguanidine on Kidney in Diabetic Nephropathy Rats. Records of Natural Products, Cilt: 12 - Sayı: 6, 602–610. https://ajindex.com/articles/308597

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