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Journals / Records of Natural Products / 2018 / Cilt: 12 - Sayı: 2

Evaluation of the in vitro Anti-inflammatory Activity of Nerium oleander L. Flower Extracts and Activity-Guided Isolation of the Active Constituents

İREM ATAY BALKAN
Ahmet C. GÖREN
HASAN KIRMIZIBEKMEZ
ERDEM YEŞİLADA
Pages
128–141
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results of the present study supported the traditional use of N. oleander flowers to treat inflammatory complaints.

📌 3 Key Takeaways

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Objective & Hypothesis:The in vitro anti-inflammatory activity profile of the Nerium oleander flower EtOH extract/its subextracts (n-hexane, CH2Cl2, EtOAc, remaining H2O) were evaluated on LPS induced Raw 264.7 macrophages.
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Key Findings & Data:The most active subextract was determined to be the EtOAc subextract without exerting any toxicity towards Raw 264.7 macrophages.
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Conclusion & Impact:Results of the present study supported the traditional use of N. oleander flowers to treat inflammatory complaints.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The in vitro anti-inflammatory activity profile of the Nerium oleander flower EtOH extract/its subextracts (n-hexane, CH2Cl2, EtOAc, remaining H2O) were evaluated on LPS induced Raw 264.7 macrophages. The effects of the crude EtOH extract and its subextracts on nitric oxide (NO) production and cell viability were determined. The most active subextract was determined to be the EtOAc subextract without exerting any toxicity towards Raw 264.7 macrophages. This subextract significantly inhibited NO production of Raw 264.7 macrophages after LPS induction (62.56±1.91% at 200 µg/mL concentration). The levels of iNOS were reduced up to 67.50%. Moreover, this subextract slightly reduced the phosphorylation levels of MAP kinases (p-ERK, p-JNK, p-38). The highest inhibition was observed for ERK phosphorylation, which was inhibited by 20.53% at 200 µg/mL concentration. Through activity-guided fractionation procedures, kaempferol, kaempferol 3-O-?-glucopyranoside and chlorogenic acid were isolated as the main active components. The structures of the active compounds were determined by 2D-NMR techniques and HRMS analysis. All compounds significantly inhibited NO productions. Results of the present study supported the traditional use of N. oleander flowers to treat inflammatory complaints.

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Cite This Article

BALKAN, İ. A., GÖREN, A. C., KIRMIZIBEKMEZ, H., YEŞİLADA, E. (2018). Evaluation of the in vitro Anti-inflammatory Activity of Nerium oleander L. Flower Extracts and Activity-Guided Isolation of the Active Constituents. Records of Natural Products, Cilt: 12 - Sayı: 2, 128–141. https://ajindex.com/articles/308292

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References (16)

  1. L.M. Coussens and Z. Werb, (2002). Inflammation and cancer, Nature 420, 860-867
  2. M. Feldmann and R.N. Maini (2003). TNF defined as a therapeutic target for rheumatoid arthritis and other autoimmune diseases, Nat. Med. 9, 1245-1250
  3. C. Gabay (2006). Interleukin-6 and chronic inflammation, Arthritis Res. Ther. 6, 1-6
  4. R. Gautam and S.M. Jachak (2009). Recent developments in anti-inflammatory natural products, Med. Res. Rev. 29,767-820
  5. R.A. Heller, M. Schena, A. Chai, D. Shalon, T. Bedilion, J. Gilmore, D.E. Woolley and R.W. Davis (1997). Discovery and analysis of inflammatory disease-related genes using cDNA microarrays, Proc. Natl. Acad. Sci. U. S. A. 94, 2150-2155
  6. B. Kaminska (2005). MAPK signalling pathways as molecular targets for anti-inflammatory therapyfrom molecular mechanisms to therapeutic benefits, Biochim. Biophys. Acta. 1754, 253-62
  7. A. Gibofsky (2012). Overview of epidemiology, pathophysiology, and diagnosis of rheumatoid arthritis, Am. J. Manag. Care. 18, S295-302
  8. A.E. M'Koma (2013). Inflammatory bowel disease: An expanding global health problem, Clin. Med. Insights Gastroenterol. 6, 33-47
  9. C. Reitz, C. Brayne and R. Mayeux (2011). Epidemiology of Alzheimer disease, Nat. Rev. Neurol. 7, 137-152
  10. P.Subbarao, P.J. Mandhane and M.R. Sears (2009). Asthma: epidemiology, etiology and risk factors, Can. Med. Assoc. J. 181, E181-190
  11. F. Kuralay and Z. Çavdar (2006). Enflamatuvar mediyatörlere toplu bakış, Genel Tıp Derg. 16, 143- 152
  12. A. Tincani, L.Andreoli, C. Bazzani, D. Bosiso and S. Sozzani (2007). Inflammatory molecules: A target for treatment of systemic autoimmune diseases, Autoimmun. Rev. 7, 1-7
  13. E. Yesilada, O. Ustün, E. Sezik, Y. Takaishi, Y. Ono and G. Honda (1997). Inhibitory effects of Turkish folk remedies on inflammatory cytokines: Interleukin-1alpha, interleukin-1beta and tumor necrosis factor alpha, J. Ethnopharmacol. 58, 59-73
  14. S.H. Tsai, S.Y. Lin-Shiau and J.K. Lin (1999). Suppression of nitric oxide synthase and the downregulation of the activation of NF?B in macrophages by resveratrol, Br. J. Pharmacol. 126, 673-680
  15. Y.C. Chen, S.C. Shen, W.R. Lee, W.C. Hou, L.L. Yang and T.J.F. Lee (2001). Inhibition of nitric oxide synthase inhibitors and lipopolysaccharide induced inducible NOS and cyclooxygenase-2 gene expressions by rutin, quercetin, and quercetin pentaacetate in RAW 264.7 macrophages, J. Cell. Biochem. 82, 537-548
  16. G.M. Cooper and E.R. Hausman (2008).