Taurochenodeoxycholic Acid Suppresses NF-κB Activation and Related Cytokines Expression in Peritoneal Macrophages from Adjuvant Arthritis Rat

TCDCA suppresses the protein and mRNA expression of TNF-α, IL-1β, and IL-6 by inhibiting the NF-κB binding activity, which is mediated through up-regulating the IκBα expression of PMs in AA rats

📌 3 Key Takeaways

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Objective & Hypothesis:Taurochenodeoxycholic acid (TCDCA) is one of main bioactive substances of animals’ bile acid and confers good anti-inflammatory activity.

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Key Findings & Data:In a definite concentration ranging from 150 μg/mL to 200 μg/mL, the over protein and mRNA expression of TNF-α, IL-1β and IL-6 were remarkably suppressed in the supernatant of PMs treatment with TCDCA.

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Conclusion & Impact:TCDCA suppresses the protein and mRNA expression of TNF-α, IL-1β, and IL-6 by inhibiting the NF-κB binding activity, which is mediated through up-regulating the IκBα expression of PMs in AA rats

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Abstract

Taurochenodeoxycholic acid (TCDCA) is one of main bioactive substances of animals’ bile acid and confers good anti-inflammatory activity. This study aimed to investigate the mechanism of the anti-adjuvant arthritis activity of TCDCA, the actions were observed in vitro on the protein and mRNA expression of cytokines, the activity of nuclear factor-kappa B (NF-κB) and its inhibitory IκBα protein level of peritoneal macrophages (PMs) in adjuvant arthritis (AA) rats. In a definite concentration ranging from 150 μg/mL to 200 μg/mL, the over protein and mRNA expression of TNF-α, IL-1β and IL-6 were remarkably suppressed in the supernatant of PMs treatment with TCDCA. Positive correlations were found between changes of NF-κB activity and expression of TNF-α, IL-1β and IL-6 influenced by TCDCA. The activity of NF-κB was markedly inhibited and IκBα protein level was increased by TCDCA (150 μg/mL,180 μg/mL and 200 μg/mL). TCDCA suppresses the protein and mRNA expression of TNF-α, IL-1β, and IL-6 by inhibiting the NF-κB binding activity, which is mediated through up-regulating the IκBα expression of PMs in AA rats

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Cite This Article

MAO, W., LİU, M., GUAN, H., LİU, B., WANG, C., HE, X., CAO, J., Lİ, P. (2018). Taurochenodeoxycholic Acid Suppresses NF-κB Activation and Related Cytokines Expression in Peritoneal Macrophages from Adjuvant Arthritis Rat. Records of Natural Products, Cilt: 12 - Sayı: 3, 263–272. https://ajindex.com/articles/306478

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