AI Summary & Key Takeaways
AI InsightWhile it is thought that such increase in MMPs would also play a role at PEX syndrome pathogenesis by leading abnormal accumulation of matrix material, the fact that such increment is not reached a meaningful value may be originated from the fact that glaucoma has not yet been developed in patients with PEX syndrome.
📌 3 Key Takeaways
Abstract
Aim: Comparison of aqueous humor levels of matrix metalloproteinase 2 (MMP-2) and 9 (MMP-9) in eyes with and without pseudoexfoliation (PEX) syndrome at aqueous humor. Material and Method: Total 30 eyes consisting of 17 eyes (Group 1) without pseudoexfoliation (PEX) syndrome and 13 eyes (Group 2), cataract surgery applied due to senile cataract and aqueous humor samples were taken from anterior chamber during surgery have been included in the study. Aqueous humor samples were kept at -80°C. Study has been performed on the levels of quantitative MMP-2 (gelatinase A) and MMP-9 (gelatinase B) by means of ELISA method. Results: While the average age of the patients was 63.4±4 years in Group 1, the average age of the patients was 61.3±2 years in Group 2 (p>0.05). Ratio of woman/man was 8/9 in Group 1, and was 7/6 in Groupt 2 (p>0.05). While MMP-2 and MMP-9 levels at humor aqueous of the eyes in Groupt 1 were 151.29 ng/ml and 12.94 ng/ml; these values at eyes with PEX syndrome were determined respectively as 205.03 ng/ml and 18.66 ng/ml. Although MMP-2 and MMP-9 levels in the eyes with PEX syndrome were found higher than those found in the control group, this difference was not statistically significant (p>0.05). Conclusion: Although gelatinized group MMPs known as predominant at aqueous humor was not statistically significant, they were determined higher as values in eyes with PEX syndrome. While it is thought that such increase in MMPs would also play a role at PEX syndrome pathogenesis by leading abnormal accumulation of matrix material, the fact that such increment is not reached a meaningful value may be originated from the fact that glaucoma has not yet been developed in patients with PEX syndrome.
Özet
Amaç: Psödoekfolyasyon (PEX) sendromu olan ve olmayan gözlerde aköz hümördeki matriks metalloproteinaz 2 (MMP-2) ve 9 (MMP-9) düzeylerinin karşılaştırılması. Gereç ve Yöntem: Senil katarakt nedeniyle katarakt cerrahisi uygulanan ve cerrahi sırasında ön kameradan aköz hümör örneği alınan psödoeksfolyasyon sendromu olmayan 17 göz (grup 1) ve olan 13 göz (grup 2) olmak üzere toplam 30 göz çalışmaya dahil edildi. Alınan aköz hümör örnekleri -80°C’de saklandı. ELISA yöntemiyle (Biotract; Amersham Buckinghamshire, UK) kantitatif MMP-2 (jelatinaz A) ve MMP-9 (jelatinaz B) düzeyleri çalışıldı. Bulgular: Hastaların yaş ortalamaları grup 1’de 63,4±4 yıl iken, grup 2’de 61,3±2 yıldı (p>0,05). Grup 1’deki hastalarda kadın/erkek oranı 8/9 iken, grup 2’de 7/6 idi p>0,05). Grup 1’deki gözlerin hümör aköz sıvısındaki MMP-2 ve MMP-9 düzeyleri 151,29 ng/ml ve 12,94 ng/ml iken; psödoeksfolyasyon sendromlu gözlerde bu değerler sırayla 205,03 ng/ml ve 18,66 ng/ml olarak saptandı. Psödoeksfolyasyon sendromlu gözlerde MMP-2 ve MMP-9 düzeyleri kontrol grubuna göre daha yüksek saptansa da bu fark istatistiksel olarak anlamlı değildi (p>0,05). Sonuç: Çalışmamızda aköz hümörde baskın olduğu bilinen jelatinaz grubu MMP’lar istatistiksel anlamlı olmasa da psödoeksfolyasyon sendromlu gözlerde daha yüksek değerlerde saptandı. MMP’lerdeki bu artışın anormal matriks materyali birikimine yol açarak PEX sendromu patogenezinde de rol oynayabileceği düşünülmekle birlikte bu artışın anlamlı değere ulaşmaması PEX sendromlu hastalarda henüz glokom gelişmemiş olmasından kaynaklanabilmektedir