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Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2015 / Cilt: 21 - Sayı: 4

Gökkuşağı Alabalıklarından (Oncorhynchus mykiss, Walbaum, 1972) İzole Edilen Aeromonas sobria Suşlarının Fenotip ve Genotip Yönünden Karşılaştırmalı Analizi

The Comparative Analysis of Phenotypic and Genotypic Properties of Aeromonas sobria Strains Isolated from Rainbow Trout (Oncorhynchus mykiss, Walbaum, 1972)

ALPER ÇİFTCİ
ERTAN EMEK ONUK
GÜLAY ÇİFTCİ
ARZU FINDIK
Mehtap Ünlü SÖĞÜT
TİMUR GÜLHAN
Pages
585–592
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, the use of double or triple combination of typing methods in this study was found to be more useful for discriminating the strains.

📌 3 Key Takeaways

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Objective & Hypothesis:Phenotypic and molecular characterization of Aeromonas sobria (A. sobria) isolates by antibiotyping, sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of whole cell proteins, random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was aimed in this study.
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Key Findings & Data:Isolates were divided into 12 different antibiotypes and 4 proteotypes according to their antibiotic susceptibilities and SDS-PAGE patterns, respectively.
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Conclusion & Impact:In conclusion, the use of double or triple combination of typing methods in this study was found to be more useful for discriminating the strains.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Phenotypic and molecular characterization of Aeromonas sobria (A. sobria) isolates by antibiotyping, sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of whole cell proteins, random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was aimed in this study. For this aim, thirty-six A. sobria isolates were analysed. Isolates were divided into 12 different antibiotypes and 4 proteotypes according to their antibiotic susceptibilities and SDS-PAGE patterns, respectively. Thirteen RAPD types were observed among all isolates. In conclusion, the use of double or triple combination of typing methods in this study was found to be more useful for discriminating the strains. The results obtained from this study may give information about phenotypic and genotypic variability of the A. sobria strains isolated from different regions of Turkey and can be helpful to control disease in fish through guiding the antibiotic therapy and giving information that will be useful to development vaccine.

Özet

Bu araştırmada Aeromonas sobria (A. sobria) izolatlarının antibiyotiplendirme, tüm hücre proteinlerinin sodyum dodesilsulfat-poliakrilamid jel electroforezi (SDS-PAGE) ile analizi, rastgele çoğaltılmış polimorfik DNA (RAPD) PCR ile genotiplendirme ile fenotipik ve genotipik karakterizasyonu amaçlanmıştır. Bu amaçla otuzaltı adet A. sobria izolatı incelenmiştir. İzolatlar antibiyotik duyarlılık sonuçlarına göre 12 farklı antibiyotip ve SDS-PAGE profiline göre 4 farklı proteotipe sahip oldukları belirlenmiştir. İzolatların 13 farklı genotipe sahip oldukları saptanmıştır. Sonuç olarak, Türkiye izolatı A. sobria suşlarının fenotipik ve genotipik olarak çeşitlilik gösterdiği ve çalışmada kullanılan tiplendirme metodlarının ikili veya üçlü kombinasyonlar halinde kullanılmasının suş ayrımında daha faydalı olduğu belirlenmiştir. Çalışmada elde edilen sonuçların balıklarda A. sobria infeksiyonlarının antibiyotik tedavi seçeneklerinin belirlenmesi konusunda faydalı olmakla birlikte, konu ile ilgili olarak yapılacak olan aşı ve teşhis kiti geliştirilmesi çalışmalarına öncülük edeceği düşünülmektedir.

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Cite This Article

ÇİFTCİ, A., ONUK, E. E., ÇİFTCİ, G., FINDIK, A., SÖĞÜT, M. Ü., GÜLHAN, T. (2015). Gökkuşağı Alabalıklarından (Oncorhynchus mykiss, Walbaum, 1972) İzole Edilen Aeromonas sobria Suşlarının Fenotip ve Genotip Yönünden Karşılaştırmalı Analizi. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 21 - Sayı: 4, 585–592. https://ajindex.com/articles/272639

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