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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2012 / Cilt: 59 - Sayı: 4

Sığır, koyun ve insanlardan izole edilen Brucella suşlarının konvansiyonel ve moleküler biyotiplendirmesi

Conventional and molecular biotyping of Brucella strains isolated from cattle, sheep and human

Alper ÇİFTCİ
Arzu FINDIK
H. Okan DOĞAN
Ahmet Bülent SÜMERKAN
Duygu PERÇİN
Fulya OCAK
Fuat AYDIN
Seçil ABAY
K. Semih GÜMÜŞSOY
Tuba İÇA
Pages
259–264
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These findings were considered to be useful in prevention and controlling activities for Brucellosis in Turkey.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, the role of Brucella spp. in cattle and sheep abortions among Kayseri region was investigated and predominant subspecies and biovars in this region were determined by conventional and molecular biotyping methods.
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Key Findings & Data:For this purpose, 61 cattle and 64 sheep abortion material and also 50 human blood isolates were examined.
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Conclusion & Impact:These findings were considered to be useful in prevention and controlling activities for Brucellosis in Turkey.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, the role of Brucella spp. in cattle and sheep abortions among Kayseri region was investigated and predominant subspecies and biovars in this region were determined by conventional and molecular biotyping methods. For this purpose, 61 cattle and 64 sheep abortion material and also 50 human blood isolates were examined. A total of 29 Brucella spp. 17 (27.9%) and 12 (18.7%) of which were isolated from cattle and sheep specimens, respectively) were isolated from animal sources. Both animal and human isolates were typed by conventional and Enhanced AMOS-ERY PCR methods. All Brucella spp. strains isolated from cattle were found to belong to B. abortus biovar 3 and biovar 3b using conventional and molecular typing methods, respectively. All sheep originated Brucella spp. strains and human originated Brucella spp. strains were found to belong to B.melitensis biovar 3 using both conventional and molecular methods. As a result, predominant biovars causing brucellosis in human, cattle and sheep in Kayseri, Turkey were detected. These findings were considered to be useful in prevention and controlling activities for Brucellosis in Turkey.

Özet

Bu çalışmada Kayseri bölgesinde sığır ve koyunlardaki atık olgularında Brucella’nın rolü araştırıldı ve ağırlıklı alt-tür ve biyovarlar konvansiyonel ve moleküler biyotiplendirme metotları ile belirlendi. Çalışmada 61 adet sığır, 64 adet koyun atık materyali ve ayrıca 50 insan kanından izole edilmiş Brucella spp. incelendi. On yedi (%27.9)’si sığır ve 12’si (%18.7) koyun atık örneklerinden olmak üzere toplam 29 adet Brucella spp. izole edildi. Hayvan ve insan izolatları konvansiyonel yöntemler ve Enhanced AMOS-ERY PCR olmak üzere iki farklı yöntem ile tiplendirildi. Çalışma kapsamında sığırlardan izole edilen tüm Brucella spp.’nin B.abortus biyovar 3’e ait olduğu konvansiyonel yöntemlerle saptandı ve bu izolatların tamamının moleküler olarak biyovar 3b olduğu tespit edildi. Koyun ve insan orijinli izolatların ise konvansiyonel ve moleküler olarak B.melitensis biyovar 3 oldukları tespit edildi. Bu çalışma ile Türkiye’nin Kayseri İli’nde, insan, sığır ve koyunlarda Brucellosis’e neden olan predominant Brucella biovarları belirlenmiş oldu. Bu bulguların Brucellosise yönelik koruma ve kontrol aktiviteleri için yararlı olabileceği düşünülmektedir.

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Cite This Article

ÇİFTCİ, A., FINDIK, A., DOĞAN, H. O., SÜMERKAN, A. B., PERÇİN, D., OCAK, F., AYDIN, F., ABAY, S., GÜMÜŞSOY, K. S., İÇA, T. (2022). Sığır, koyun ve insanlardan izole edilen Brucella suşlarının konvansiyonel ve moleküler biyotiplendirmesi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 59 - Sayı: 4, 259–264. https://ajindex.com/articles/444888

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References (15)

  1. Al-Ani FK, Qaderi NQ, Razziq R, Al-Darraji AM (2004): Human and animal brucellosis in Jordan between 1996–1998: a study. Rev Sci Tech Off Int Epizoot, 23, 831–840
  2. Alton GG, Jones LM, Pietz DE (1975): Laboratory Techniques in Brucellosis. Monograph Series. World Health Organization, Geneva, Switzerland
  3. Anon (2011): WHO, Brucellosis, http://www.who.int/ zoonoses/diseases/ Brucellosissurveillance.pdf
  4. Bolca Z, Gündeş S, Erdenliğ S, Özturk R, Sümerkan B, Akata, F, Vahaboğlu, H (2002): İnsan kaynaklı brusella türü mikroorganizmaların tiplendirilmesi amacı ile uygulanan metotların karşılaştırılması ve biyotipleri ile faj tipleri arasındaki ilişkinin irdelenmesi. Flora, 7(3), 157– 170
  5. Bricker BJ (2002a): Diagnostic Strategies Used for the Identification of Brucella. Vet Microbiol, 90, 433–434
  6. Bricker BJ (2002b): PCR as a Diagnostic Tool for Brucellosis. Vet Microbiol, 90, 435–446
  7. Bricker BJ, Halling SM (1994): Differentiation of Brucella abortus by 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol, 32, 2660–2666
  8. Bricker BJ, Halling SM (1995): Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51. J Clin Microbiol, 33, 1640–1642
  9. Buzgan T, Karahocagil MK, Irmak H (2010): Clinical manifestations and complications in 1028 cases of brucellosis: a retrospective evaluation and review of the literature. Int J Infect Dis, 14, 469–478
  10. Büyük, F, Şahin M (2011): Invesigation of Brucella species from various samples of aborted cattle in Kars Province (Turkey) by cultural and molecular methods and epidemiological analysis of cases, The Journal of the Faculty of Veterinary Medicine, University of Kafkas, 17 (5), 809–816
  11. Büyükcangaz E, Şen A, Kahya S (2009): Isolation and Biotyping of Brucella melitensis from Sheep and Goat Aborted Fetuses. Turk J Vet Anim Sci, 33, 311–316
  12. Çelebi Ö, Otlu S (2011): Bacteriological and Molecular Description of Brucella SpeciesIsolated from Milk and Vaginal Swab Samples of Aborted Cattlein Kars Region.The Journal of the Faculty of Veterinary Medicine, University of Kafkas, 17 (1), 53–58
  13. Doğanay M, Aygen B (2003): Human Brucellosis: an overview, Int J Infect Dis, 7, 173–182
  14. Erdenliğ S, Sen A (2000): Isolation and biotyping of Brucella species in aborted sheep fetuses. J Pendik Vet Microbiol, 31, 31–42
  15. Güler L, Gündüz K, Ok U (2003): Comparison of polymerase chain reaction and bacter