Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2016 / Cilt: 22 - Sayı: 3

Deniz Ürünlerinde Salmonella spp. ve Listeria monocytogenes Varlığının Kültürel Metotlar ve PCR İle Araştırılması

Investigation of Salmonella spp. and Listeria monocytogenes in Seafood by Cultural Methods and PCR [1]

Pages
397–401
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Therefore, it is essential to ensure improving the quality of production technology from fishing to retail outlet and developing the sanitation conditions of food contact surfaces and handling areas.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The present study was conducted to investigate the presence of Salmonella spp. and Listeria monocytogenes in 700 seafood (400 raw fish, 100 raw shrimps and 200 raw molluscs) collected from retailers.
📊
Key Findings & Data:Salmonella spp. and L. monocytogenes were detected in 9.9% and 3.86% fish and shellfish samples, respectively.
💡
Conclusion & Impact:Therefore, it is essential to ensure improving the quality of production technology from fishing to retail outlet and developing the sanitation conditions of food contact surfaces and handling areas.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The present study was conducted to investigate the presence of Salmonella spp. and Listeria monocytogenes in 700 seafood (400 raw fish, 100 raw shrimps and 200 raw molluscs) collected from retailers. Isolations were performed by conventional culture methods. The isolates were also confirmed by PCR assays. Salmonella spp. and L. monocytogenes were detected in 9.9% and 3.86% fish and shellfish samples, respectively. The highest rates of Salmonella spp. (12.5%) were found in fish samples and L. monocytogenes (7.0%) were in shrimp samples. Therefore, it is essential to ensure improving the quality of production technology from fishing to retail outlet and developing the sanitation conditions of food contact surfaces and handling areas.

Özet

Bu çalışma perakendecilerden toplanan 700 deniz ürününde (400 çiğ balık, 100 çiğ karides ve 200 çiğ molüsk (yumuşakça)) Salmonella spp. and Listeria monocytogenes varlığının araştırılması amacıyla yapıldı. İzolasyonda konvansiyonel kültür metotları kullanıldı ve tüm izolatların PCR ile konfirmasyonları gerçekleştirildi. Balık ve kabuklu deniz hayvanlarından Salmonella spp. %9.9 ve L. monocytogenes %3.86 oranında belirlendi. Salmonella spp. en yüksek oranda balık örneklerinde (%12.5) saptanırken L. monocytogenes ise karides örneklerinde (%7.0) saptandı. Bu çalışmanın sonuçları, avlanması/yetiştirilmesinden perakende satış aşamasına kadar üretim teknolojisinin kalitesinin iyileştirilmesinin ve gıda temas yüzeyleri ile ambalajlama alanlarında sanitasyon koşullarının geliştirilmesinin gerekliliğinin önemini göstermektedir.

🔖

Cite This Article

İKİZ, S., DUMEN, E., KAHRAMAN, B. B., BAYRAKAL, G. M., KAHRAMAN, T., ERGİN, S. (2016). Deniz Ürünlerinde Salmonella spp. ve Listeria monocytogenes Varlığının Kültürel Metotlar ve PCR İle Araştırılması. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 22 - Sayı: 3, 397–401. https://ajindex.com/articles/272483

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (9)

  1. Venugopal V: Seafood Processing: Adding Value Through Quick Freezing, Retortable Packaging and Cook-chilling. CRC Press, Taylor & Francis Group, Florida, USA, 2006.
  2. Nollet LML, Toldra F: Seafood and Seafood Product Analysis. CRC press, Taylor & Francis Group, Florida, USA, 2010.
  3. Setti I, Rodriguez-Castro A, Pata MP, Cadarso-Suarez C, Yacoubi B, Bensmael L, Moukrim A, Martinez-Urtaza J: Characteristics and dynamics of Salmonella contamination along the coast of Agadir, Morocco. Appl Environ Microbiol, 75, 7700-7709, 2009. DOI: 10.1128/ AEM.01852-09
  4. Martinez-Urtaza J, Saco M, de Novoa J, Perez-Pieiro P, Peiteado J, Lozano-Leon A, Garcia-Martin O: Influence of environmental factors and human activity on the presence of Salmonella serovars in a marine environment. J Appl Environ Microbiol, 70, 2089-2097, 2004. DOI: 10.1128/ AEM.70.4.2089-2097.2004
  5. Van TT, Moutafis G, Istivan T, Tran LT, Coloe PJ: Detection of Salmonella spp. in retail raw food samples from Vietnam and characterization of their antibiotic resistance. Appl Environ Microbiol, 73, 6885-6890, 2007. DOI: 1128/AEM.00972-07
  6. Kumar R, Surendran PK, Thampuran N: Evaluation of culture, ELISA and PCR assays for the detection of Salmonella in seafood. Lett Appl Microbiol, 46, 221-226, 2008. DOI: 10.1111/j.1472-765X.2007.02286.x Herrera FC, Santos JA, Otero A, Garcia-Lopez MA: Occurrence of foodborne pathogenic bacteria in retail prepackaged portions of marine fish in Spain. J Appl Microbiol, 100, 527-536, 2006. DOI: 10.1111/j.1365- 2005.02848.x Draughon FA, Anthony BA, Denton ME: Listeria species in fresh rainbow trout purchased from retail markets. Dairy Food Environ Sanit,, 90-94, 1999.
  7. Karunasagar I, Segar K, Karunasagar I, Goebel W: Incidence of Listeria spp. in tropical seafoods. In, Listeria 1992. Abstract No. 155. Eleventh International Symposium on Problems of Listeriosis (ISOPOL XI), May I lth-14th, Copenhagen, Denmark, 1992.
  8. Jorgensen LV, Huss HH: Prevalence and growth of Listeria monocytogenes in naturally contaminated seafood. Int J Food Microbiol,, 127-131, 1998. DOI: 10.1016/S0168-1605(98)00071-3
  9. Zarei M, Maktabi S, Ghorbanpour M: Prevalence of Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, and Salmonella spp. in seafood products using multiplex polymerase chain reaction. Foodborne Pathog Dis, 9, 108-112, 2012. DOI: 10.1089/ fpd.2011.0989 Can MF, Günlü A, Can HY: Fish consumption preferences and factors influencing it. Food Sci Technol (Campinas), 35, 339-346, 2015.