Journals / İstanbul Üniversitesi Veteriner Fakültesi Dergisi / 2012 / Cilt: 38 - Sayı: 2

Species distribution of methicillin resistant staphylococci isolated from animals, environmental samples and staffs

Hayvanlardan, klinik ortamından ve klinik çalışanlarından izole edilen metisiline dirençli stafilokokların tür dağılımları

Pages
151–160
Publication Date
2022-10-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Out of 41isolates 87.7%, 63.4%, 58.5% of them were resistant to penicillin G, erythromycin and tetracycline, respectively.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of this study is to investigate the presence of methicillin resistant staphylococci, particularly methicillinresistant Staphylococcus aureus (MRSA), from the dogs and cats that brought to the Veterinary Faculty clinics and thestaff in those clinics, and risk of the environmental contaminations in the clinics.
📊
Key Findings & Data:For this purpose; swab samples werecollected from nasal mucosa of 18 staffs, nasal and oral mucosa of 7 cats and 21 dogs.
💡
Conclusion & Impact:Out of 41isolates 87.7%, 63.4%, 58.5% of them were resistant to penicillin G, erythromycin and tetracycline, respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study is to investigate the presence of methicillin resistant staphylococci, particularly methicillinresistant Staphylococcus aureus (MRSA), from the dogs and cats that brought to the Veterinary Faculty clinics and thestaff in those clinics, and risk of the environmental contaminations in the clinics. For this purpose; swab samples werecollected from nasal mucosa of 18 staffs, nasal and oral mucosa of 7 cats and 21 dogs. For determination of environmental contamination 33 swab samples from various surfaces of the clinics were collected. Gram positive cocciwere identified following after the determination of methicillin resistance by disc diffusion method. All the isolatesexamined for the presence of the mecA gene by PCR, for molecular typing by RAPD-PCR. Antibiotic susceptibilitiesof the isolates were determined. Three (42.9%), 5 (23.8%), 19 (57.6%) and 13 (72.2%) methicillin resistant coagulasenegative staphylococci (MRCoNS) were isolated out of 7 cats, 21 dogs, 33 environmental and 18 staff samples,respectively. While S. hominis were isolated predominantly, no MRSA were isolated from the samples. Out of 41isolates 87.7%, 63.4%, 58.5% of them were resistant to penicillin G, erythromycin and tetracycline, respectively.

Özet

Bu çalışmada, Veteriner fakültesi kliniklerinde görevli personelde ve kliniklere getirilen kedi ve köpeklerde, baştaStaphylococcus aureus olmak üzere metisiline dirençli diğer stafilokokların varlığının ve kliniklerde çevresel kaynaklıbir kontaminasyon riskinin araştırılması amaçlandı. Bu amaçla; fakülte kliniklerinde çalışan personelden 18 kişidennazal svab örnekleri, bu kliniklere getirilen 7 kedi ve 21 köpeğin nazal ve oral svab örnekleri toplandı. Çevreselkontaminasyonu saptamak için kliniklerde farklı yüzeylerden toplam 33 svab örneği toplandı. Bakteriyolojik incelemesonucu izole edilen Gram pozitif kokların, disk diffuzyon yöntemi ile metisiline direnç durumları saptandı ve ardındanidentifikasyonları yapıldı. Tüm izolatlar mecA geni yönünden PCR ile moleküler tiplendirme amacıyla da RAPD-PCRile incelendi. Ayrıca izolatların antibiyotiklere duyarlılıkları saptandı. Yedi kedinin 3 (%42,9)’ünden, 21 köpeğin 5(%23,8)’inden; 33 çevresel örneğin 19 (%57,6)’undan; personelden toplanan 18 svab örneğinin 13 (%72,2)’ündenmetisiline dirençli koagulaz negatif stafilokoklar (MRCoNS) izole edildi. Örneklerin hiçbirinden metisiline dirençli S.aureus (MRSA) izole edilmezken, her iki örnek grubunda da en yüksek oranda S. hominis izole edildi. Toplam 41izolatın %87,8’inde penisilin G direnci, %63,4’ünde eritromisin direnci, %58,5’inde tetrasiklin direnci saptandı.

🔖

Cite This Article

BAĞCIGİL, A. F., GÜZEL, Ö., İKİZ, S., YARAMIŞ, Ç. P., ILGAZ, A. (2022). Species distribution of methicillin resistant staphylococci isolated from animals, environmental samples and staffs. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 38 - Sayı: 2, 151–160. https://ajindex.com/articles/555187

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Armand-Lefevre, L., Ruimy, R., Andremont, A., 2005. Clonal comparison of Staphylococcus aureus isolates from healthy pig farmers, human control, and pigs. Emerging Infectious Diseases 11 (5), 711-714.
  2. Bagcigil, F.A., Moodley, A., Baptiste, K.E., Jensen, V.F., Guardabassi, L., 2007. Occurence, species distrubution, antimicrobial resistance and clonality of methicillin- and erythromycin- resistant staphylococci in the nasal cavity of domestic animals. Veterinary Microbiology 121 (3-4), 307-315.
  3. Bogado, I., Limansky, A., Sutich, E., Marchiaro, P., Marzi, M., Putero J., Viale, A., 2002. Molecular characterization of methicillin- resistant coagulase-negative staphylococci from a neonatal intensive care unit. Infection Control and Hospital Epidemiology 23 (8), 447-451.
  4. Boost, M.V., O’Donoghue, M.M., James, A., 2008. Prevalence of Staphylococcus aureus carriage among dogs and their owners. Epidemiology and Infection 136 (7), 953-964.
  5. Busscher, J.F., van Duijkeren, E., van Oldruitenborgh-Oosterbaan, S., 2006. The prevalence of methicillin-resistant staphylococci in healthy horses in the Netherlands. Veterinary Microbiology 113 (1- 2), 131-136.
  6. Duquette, R.A., Nuttall, T.J., 2004. Methicillin- resistant Staphylococcus aureus in dogs and cats: an emerging problem? Journal of Small Animal Practice 45 (12), 591- 597. Epstein, C.R., Yam, W.C., Peiris, J.S.M., Epstein, R.J., 2009. Methicillin-resistant commensal staphylococci in healthy dogs as a potential zoonotic reservoir for community-acquired antibiotic resistance. Infection, Genetics and Evolution 9 (2), 283-285.
  7. Fındık, A., Akan, N., Onuk, E.E., Çakıroğlu, D., Çiftçi, A., 2009. Methicillin resistance profile and molecular typing of Staphylococcus aureus strains isolated from noses of the healthy dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 15 (6), 925-930.
  8. Gortel, K., Campbell, K.L., Kakoma, I., Whittem, T., Schaeffer, D.J., Weisiger, R.M., 1999. Methicillin resistance among staphylococci isolated from dogs. American Journal of Veterinary Research 60 (12), 1526-1530.
  9. Jonas, D., Speck, M., Daschner, F.D., Grundmann, H., 2002. Rapid PCR-based identification of methicillin-resistant Staphylococcus aureus from screening swabs. Journal of Clinical Microbiology 40 (5), 1821-1823
  10. Kitao, T., 2003. Survey of methicillin-resistant coagulase negative staphylococci isolated from the fingers of nursing students. Journal of Infection and Chemotheraphy 9 (1), 30-34.
  11. Koneman, E.W., Allen, S.D., Janda, W.M., Schreckenber