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Journals / İzmir Dr. Behçet Uz Çocuk Hastanesi Dergisi / 2018 / Cilt: 8 - Sayı: 1

Investigation of changes in vWF expression in rabbit carotid artery collar model

Tavşan karotis arteri yaka modelinde vWF ekspresyonu değişimlerinin incelenmesi

MEHMET ZUHURİ ARUN
Gülnur SEVİN
Günay Yetik ANACAK
Ceren GÖNEN KORKMAZ
Levent ÜSTÜNES
Pages
37–42
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Our findings emphasize the importance of vWF in collar-induced intimal thickening.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Placement of a soft silicone collar around the carotid arteries of rabbits induces intimal thickening which is considered as one of the crucial steps in the development of atherosclerosis.
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Key Findings & Data:In this study we aimed to investigate vWF expression levels on days 7 and 14 after collar placement.
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Conclusion & Impact:Conclusion: Our findings emphasize the importance of vWF in collar-induced intimal thickening.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Placement of a soft silicone collar around the carotid arteries of rabbits induces intimal thickening which is considered as one of the crucial steps in the development of atherosclerosis. In this study we aimed to investigate vWF expression levels on days 7 and 14 after collar placement. Methods: A soft inert silicon collar was placed around the left carotid artery for 7 and 14 days. vWF expression was investigated by quantitave RT-PCR. In each artery (collar or sham) cross-sectional areas of intima, media were measured and intima/ media ratio was calculated. Results: Intimal thickening was more pronounced on day 14 compared to day 7. However the collar caused increase in vWF expression which was found to be at the same level on days 7 and 14. Conclusion: Our findings emphasize the importance of vWF in collar-induced intimal thickening.

Özet

Amaç: Tavşan karotis arteri çevresine yaka yerleştirilmesi ateroskleroz gelişiminin en önemli basamaklarından biri olan intimal kalınlaşmaya neden olur. Bu çalışmada, yaka modelinde vWF ekspresyon düzeylerinin yedinci ve on dördüncü günde incelenmesi amaçlanmıştır. Yöntem: İnert, yumuşak silikon yaka sol karotis arter çevresine yedi veya on dört gün süre ile yerleştirilmiştir. vWF ekspresyonu RT-PCR yöntemi ile incelenmiştir. Her bir arterin intima/medya (indeks) oranları ölçülmüştür. Bulgular: Yedinci gün ile on dördüncü gün kıyaslandığında indeks değerinin on dördüncü günde daha da arttığı görülmüştür. Yaka vWF ekspresyon düzeyinde artışa neden olmuştur. Yakanın neden olduğu vWF düzeylerindeki artış incelenen her iki günde de aynı düzeyde bulunmuştur. Sonuç: Elde ettiğimiz bulgular yaka modeli ile oluşan intimal kalınlaşmada vWF’ün önemini vurgulamaktadır.

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Cite This Article

ARUN, M. Z., SEVİN, G., ANACAK, G. Y., KORKMAZ, C. G., ÜSTÜNES, L. (2018). Investigation of changes in vWF expression in rabbit carotid artery collar model. İzmir Dr. Behçet Uz Çocuk Hastanesi Dergisi, Cilt: 8 - Sayı: 1, 37–42. https://ajindex.com/articles/268683

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References (9)

  1. Chistiakov DA, Myasoedova VA, Melnichenko AA, Grechko AV, Orekhov AN. Calcifying Matrix Vesicles and Atherosclerosis. BioMed Research International. 2017;2017:7463590. https://doi.org/10.1155/2017/7463590 Hansson GK. Inflammation, atherosclerosis, and coronary artery disease. The New England Journal of Medicine. 2005;352(16):1685-95. https://doi.org/10.1056/NEJMra043430
  2. Lv JX, Kong Q, Ma X. Current advances in circulating inflammatory biomarkers in atherosclerosis and related cardiocerebrovascular diseases. Chronic Diseases and Translational Medicine. 2017;3(4):207-12. https://doi.org/10.1016/j.cdtm.2017.09.002
  3. De Meyer GR, Hoylaerts MF, Kockx MM, Yamamoto H, Herman AG, Bult H. Intimal deposition of functional von Willebrand factor in atherogenesis. Arteriosclerosis, Thrombosis, and Vascular Biology. 1999;19(10):2524-34. https://doi.org/10.1161/01.ATV.19.10.2524 Newby AC. Dual role of matrix metalloproteinases (matrixins) in intimal thickening and atherosclerotic plaque rupture. Physiological Reviews. 2005;85(1):1-31. https://doi.org/10.1152/physrev.00048.2003
  4. Getz GS, Reardon CA. Animal models of atherosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology. 2012;32(5):1104-15. https://doi.org/10.1161/ATVBAHA.111.237693
  5. Booth RF, Martin JF, Honey AC, Hassall DG, Beesley JE, Moncada S. Rapid development of atherosclerotic lesions in the rabbit carotid artery induced by perivascular manipulation. Atherosclerosis. 1989;76(2-3):257-68. https://doi.org/10.1016/0021-9150(89)90109-3
  6. De Meyer GR, Van Put DJ, Kockx MM, Van Schil P, Bosmans R, Bult H, et al. Possible mechanisms of collarinduced intimal thickening. Arteriosclerosis, Thrombosis, and Vascular Biology. 1997;17(10):1924-30. https://doi.org/10.1161/01.ATV.17.10.1924
  7. Arun MZ, Ustunes L, Sevin G, Ozer E. Effects of vitamin C treatment on collar-induced intimal thickening. Drug Design, Development and Therapy. 2015;9:6461-73. https://doi.org/10.2147/DDDT.S97020
  8. Kockx MM, De Meyer GR, Jacob WA, Bult H, Herman AG. Triphasic sequence of neointimal formation in the cuffed carotid artery of the rabbit. Arteriosclerosis and Thrombosis: a Journal of Vascular Biology. 1992;12(12):1447-57. https://doi.org/10.1161/01.ATV.12.12.1447
  9. Van Put DJ, Van Osselaer N, De Meyer GR, Andries LJ, Kockx MM, De Clerck LS, et al. Role of polymorphonuclear leukocytes in collar-induced intimal thickening in the rabbit carotid artery. Arteriosclerosis, Thrombosis, and Vascular Biology. 1998;18(6):915-21. https://doi.org/10.1161/0