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Journals / İzmir Dr. Behçet Uz Çocuk Hastanesi Dergisi / 2020 / Cilt: 10 - Sayı: 2

Role of Calretinin in Determining the Aganglionic ID Segment in Hirschsprung’s Disease

Hirschsprung Hastalığında Aganglionik Segmentin Belirlenmesinde Kalretinin’in Rolü

Onur CEYLAN
Özgür ÇAĞLAR
Pages
164–169
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Calretinin was evaluated as negative in 60 of aganglionic biopsy materials whereas it was found to be weakly cytoplasmic positive in three cases.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: This study aims to determine the presence, shape, and value of immunoreactivity of calretinin in ganglionic and aganglionic colon samples in Hirschsprung’s Disease (HSCR).Method: This study included the ganglion positive and negative intestinal tissue materials of 63 patients admitted to the hospital with the prediagnosis of HSCR.
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Key Findings & Data:Calretinin was evaluated as negative in 60 of aganglionic biopsy materials whereas it was found to be weakly cytoplasmic positive in three cases.
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Conclusion & Impact:A significant difference was observed between the positive immunoreactivity of calretinin in the aganglionic and ganglionic segments (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: This study aims to determine the presence, shape, and value of immunoreactivity of calretinin in ganglionic and aganglionic colon samples in Hirschsprung’s Disease (HSCR).Method: This study included the ganglion positive and negative intestinal tissue materials of 63 patients admitted to the hospital with the prediagnosis of HSCR. Calretinin immunomarker was applied to the materials. The cases were divided into three groups as strongly positive, weakly positive and negative for calretinin. Staining differences between ganglionic and aganglionic tissue samples were examined.Results: In all ganglionic biopsy materials, calretinin was seen to be strongly positive in the submucosal and myenteric plexuses. Calretinin was evaluated as negative in 60 of aganglionic biopsy materials whereas it was found to be weakly cytoplasmic positive in three cases. A significant difference was observed between the positive immunoreactivity of calretinin in the aganglionic and ganglionic segments (p

Özet

Amaç: Çalışmamızın amacı Hirschsprung Hastalığında (HH) gangliyonik ve agangliyonik kolon örneklerinde kalretininin immünreaktivitesinin varlığını, şeklini ve değerini belirlemektir. Yöntem: HH ön tanısı ile gelen 63 olgunun gangliyon pozitif ve negatif bağırsak dokusu tanısı alan materyalleri çalışmaya dâhil edilip kalretininin immünbelirteci uygulandı. Kalretinin için vakalar kuvvetli pozitif, zayıf pozitif ve negatif olarak üç gruba ayrıldı. Gangliyon içeren doku örnekleri ile gangliyon içermeyen doku örnekleri arasındaki boyanma farklılıkları incelendi. Bulgular: Gangliyon içeren biyopsi materyallerinin tamamında submukozal ve miyenterik pleksuslardaki gangliyonlarda kalretinin kuvvetli pozitif olarak izlendi. Gangliyon içermeyen biyopsilerin ise 60’ında negatif, 3’ünde zayıf stoplazmik pozitif olarak değerlendirildi. Agangliyonik ve gangliyonik segmentlerde kalretinin pozitif immünreaktivitesi arasında anlamlı bir farklılık izlendi (p

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Cite This Article

CEYLAN, O., ÇAĞLAR, Ö. (2022). Role of Calretinin in Determining the Aganglionic ID Segment in Hirschsprung’s Disease. İzmir Dr. Behçet Uz Çocuk Hastanesi Dergisi, Cilt: 10 - Sayı: 2, 164–169. https://ajindex.com/articles/652195

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