Journals / Hacettepe Journal of Biology and Chemistry / 2015 / Cilt: 43 - Sayı: 1

Investigation of Biofilm Formation in Clinically Acquired Escherichia coli Strains

Klinik Kaynaklı Escherichia coli Suşlarında Biyofilm Oluşumunun Araştırılması

Pages
43–48
Publication Date
2015-03-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Accordingly, NBF strains of E. coli were more susceptible to eight antibiotics than Strong Biofilm Former SBF strains.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, investigation of biofilm formation levels of clinically acquired Escherichia coli strains and exami- nation of each E. coli strains’ clinical information such as clinical material and service units according to their biofilm formation results were determined.
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Key Findings & Data:In this respect, according to our results; E. coli strains are grouped as 31% Strong Biofilm Former SBF , 27% Intermediate Biofilm Former IBF , 25 % Weak Biofilm Former WBF and 17% None Biofilm Former NBF .
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Conclusion & Impact:Accordingly, NBF strains of E. coli were more susceptible to eight antibiotics than Strong Biofilm Former SBF strains.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Biyofilm oluşumu · Escherichia coli · antibiyotik hassasiyeti

Abstract

In this study, investigation of biofilm formation levels of clinically acquired Escherichia coli strains and exami- nation of each E. coli strains’ clinical information such as clinical material and service units according to their biofilm formation results were determined. In this respect, according to our results; E. coli strains are grouped as 31% Strong Biofilm Former SBF , 27% Intermediate Biofilm Former IBF , 25 % Weak Biofilm Former WBF and 17% None Biofilm Former NBF . In addition to this, clinical materials of wound and urine were found as the most frequent clinical materials from which strong biofilm Former E. coli strains isolated. Besides, urology and cardiology were found as the most SBF isolated service units. Apart from these, E. coli strains were mostly isolated from urinary tract infections and from women who are at the period of post-menopausal. Lastly, the antibiotic susceptibility patterns were investigated and the greatest susceptibility was observed against amika- cin and the least susceptibility was observed against trimethoprim/sulfamethoxazole. Accordingly, NBF strains of E. coli were more susceptible to eight antibiotics than Strong Biofilm Former SBF strains.

Özet

B u çalışmada, klinik materyallerden elde edilen Escherichia coli suşlarının biyofilm oluşturma yetenekleri araştırıldı ve her bir E. coli suşunun klinik materyal ve servis ünitesi bilgileri biyofilm oluşturma sonuçları ile bir arada değerlendirildi. Bu bağlamda çalışmamızın sonuçlarına göre E. coli suşlarının %31’i Yüksek Biyofilm Oluşturan YBF , %27’si Ilımlı Biyofilm Oluşturan IBO , %25’i Zayıf Biyofilm Oluşturan ZBF ve %17’si ise Biyofilm Oluşturmayan olarak gruplandırıldı. Buna ek olarak klinik materyallerden yara ve idrarın en yüksek sıklıkta izole edilen klinik materyaller olduğu saptandı. Ayrıca Yüksek Biyofilm Oluşturan YBO suşların en fazla üroloji ve kardiyoloji servislerinden izole edildiği gözlendi. Bunların yanı sıra, E. coli suşlarının post-menapozal dönemdeki üriner yol enfeksiyonu geçiren kadınlardan en fazla sıklıkta izole edildiği saptandı. Son olarak E. coli suşlarının antibiyotik hassaslık paternleri incelendiğinde en yüksek hassaslığın amikasin antibiyotiğine, en düşük hassaslığın ise trimetoprim sülfametaksazol antibiyotiğine karşı olduğu gözlendi. Bununla ilişkili olarak Biyofilm Oluşturmayan E. coli suşlarının çalışmada kullanılan 8 antibiyotiğe karşı Yüksek Biyofilm Oluşturan YBF E. coli suşlarına kıyasla daha hassas oldukları belirlendi

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Cite This Article

AVCIOĞLU, N. H., ŞAHAL, G., BİLKAY, I. S. (2015). Investigation of Biofilm Formation in Clinically Acquired Escherichia coli Strains. Hacettepe Journal of Biology and Chemistry, Cilt: 43 - Sayı: 1, 43–48. https://ajindex.com/articles/255977

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References (13)

  1. S.M. Soto, A. Smithson, J. A. Martinez, J. P. Horcajada, J. Mensa and J. Vila, Biofilm formation in uropathogenic Escherichia coli strains: Relationship with prostatitis, urovirulence factors and antimicrobial resistance, The Journal of Urology, 177 (2007) 365-368
  2. J. Rodríguez-Bano, E. Pico´n, P. Gijo´n, J.R. Herna´ndez, J. M. Cisneros, C. Pena, M. Almela, B. Almirante, F. Grill,J. Colomina, S.Molinos, A. Oliver, C. Ferna´ndezMazarrasa, G.Navarro, A.Coloma, L. Lo´pez-Cerero, A. Pascual, Risk factors and prognosis of nosocomial bloodstream infections caused by extendedspectrum-lactamase-producing Escherichia coli, Journal of Clinical Microbiology, 48 (2010) 1726–1731
  3. C.A. Fux, J.W. Costerton, P.S. Stewart, P. Stoodley, Survival strategies of infectious biofilms, Trends in Microbiology, 13 (2005) 34-40
  4. R. Dewanti, A.M.C.L. Wong, Influence of culture conditions on biofilm formation by Escherichia coli 0157:H7, International Journal of Food Microbiology, 26 (1995) 147-164
  5. C.A. Bopp, F.W. Brenner, J.G. Wells, N. Strockbin, Escherichia, Shigella and Salmonella. Manual Clin. Microbiol.,7 (1999) 459–474
  6. G.A. O’Toole, Microtiter dish biofilm formation assay, J Vis Exp., 30(2011) 2437
  7. J.W. Costerton, P.S. Stewart, E.P. Greenberg, Bacterial biofilms: a common cause of persistent infections, Science, 284 (1999), 1318-1321
  8. P. Naves, G. Prado, L. Huelves, V. Rodríguez-Cerrato, V. Ruiz, M.C. Ponte, F. Soriano Effects of human serum albumin, ibuprofen and N-acetyl-L-cysteine against biofilm formation by pathogenic Escherichia coli strains, Journal of Hospital Infection, 76 (2010) 165-170
  9. A. Reisner, J.A.J. Haagensen, M.A. Schembri, E.L. Zechner, S.Molin, Development and maturation of Escherichia coli K-12 biofilms, Molecular Microbiology, 48 (2003) 933–946
  10. L.F. Melo, M.J. Vieira, Physical stability and biological activity of biofilms under turbulent flow and low substrate concentration, Bioprocess Eng., 20 (1999) 363–368
  11. J.M.R. Moreira, L.C. Gomes, J.D.P. Araujo, J.M. Miranda, M. Simoes, L.F. Melo, F.J. Mergulhao, The effect of glucose concentration and shaking conditions on Escherichia coli biofilm formation in microtiter plates, Chemical Engineering Science, 94 (2013) 192–199
  12. D.C. Bean, D. Krahe, D.W. Wareham, Antimicrobial resistance in community and nosocomial Escherichia coli urinary tract isolates, London 2005–2006, Annals of Clinical Microbiology and Antimicrobials, 7 (2008) 1-4
  13. C. Beloin, A. Roux, J.M. Ghi