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Journals / Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi / 1998 / Cilt: 3 - Sayı: 3

Cloning of hepatitis B virus surface gene region to Escherichia coli

Hepatit B virüs yüzey antijen (surface) gen bölgesinin Escherichia coli' ye klonlanması

Ayhan KUBAR
Mehmet YAPAR
MUSTAFA ÖZYURT
Tunçer HAZNEDAROĞLU
Hüseyin GÜN
Pages
183–186
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our aim was to achieve gene transfer via general principles of recombinant DNA technologies and to use the cloned DNA fragments in future expression studies to obtain antigens to be utilized for diagnostic and immunization procedures.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, we cloned HBV s gene to E coli by using PCR-dependent TA cloning procedure.
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Key Findings & Data:In this study, we cloned HBV s gene to E coli by using PCR-dependent TA cloning procedure.
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Conclusion & Impact:Our aim was to achieve gene transfer via general principles of recombinant DNA technologies and to use the cloned DNA fragments in future expression studies to obtain antigens to be utilized for diagnostic and immunization procedures.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, we cloned HBV s gene to E coli by using PCR-dependent TA cloning procedure. Our aim was to achieve gene transfer via general principles of recombinant DNA technologies and to use the cloned DNA fragments in future expression studies to obtain antigens to be utilized for diagnostic and immunization procedures.

Özet

Biz bu çalışmada HBV'nin yüzey antijen (surface) genini "PCR-dependent TA cloning" yöntemi ile E coli'ye klonladık. Amacımız rekombinant DNA teknolojisinin genel prensiplerini kullanarak gen aktarımlarını gerçekleştirmek ve ileride elde edeceğimiz ürünleri hastalıkların korunma (aşı) ve tanısında (antijen) kullanmaktır.

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Cite This Article

KUBAR, A., YAPAR, M., ÖZYURT, M., HAZNEDAROĞLU, T., GÜN, H. (1998). Cloning of hepatitis B virus surface gene region to Escherichia coli. Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi, Cilt: 3 - Sayı: 3, 183–186. https://ajindex.com/articles/224394

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References (1)

  1. Ganem D. Hepadnaviridae and their replication In: Fi-elds BN, Knipe DM, Howley PM, et al (eds). Fields Viro-logy. Third edition, Philadelphia: Lippincott-Raven Pub-lishers. 1996;2703-37. 2.Beasley RP. Hepatitis B virus-the major etiology of hepa-tocellular carcinoma. Cancer 1988;61:1942-56. 3.Ganem D. Persistent infection of humans with hepatitisB virus: mechanisms and consequences. Rev Infect Dis1982;4:1026-47. 4.Hollinger FB. Hepatitis B virus In: Fields BN, Knipe DM,Howley PM, et al (eds). Fields Virology. Third edition,Philadelphia: Lippincott-Raven Publishers, 1996;2739-807. 5.Peters RL. Viral hepatitis: a patologic spectrum. Am JMed Sci 1975;270:17-28. 6.Redeeker AD. Viral hepatitis: clinical aspects. Am J Med Sci 1975;270:9-16. 7.Attanasio-R Lanford RE, Dilley-D, Stunz GW, Notvall L,Henderson AB, Kennedy RC. Immunogenicity of hepa-titis B surface antigen derived from the baculovirus exp-ression vector system: a mouse potency study. Biologi-cals 1991;19(4):347-53. 8.Cregg JM, Tschopp JF, Stillman C, et al. High level exp-ression and efficient assembly of hepatitis B surface an-tigen in the methylotrophic yeast, Pichia pastoris.Bio/Technology 1987;5:479-85. 9. Fujisawa Y, Ito Y, Ikeyama S, Kikuchi M. Expression ofhepatitis B virus surface antigen P31 gene in Escheric-hia coli. Gene 1985;40:23-9. 10. Hamsa PV, Chattoo BB. Cloning and growth-regulatedexpression of the gene encoding the hepatitis B virusmiddle surface antigen in Yarrowia lipolytica. Gene1994;143(2):165-70. 11. Mason HS, Lam DM, Arntzen CJ. Expression of hepati-tis B surface antigen in transgenic plants. SO: Proc-Natl-Acad-Sci-USA 1992;89(24):11745-9. 12. Pumpen P, Kozlovskaya TM, Borisova GP, Bichko VV,Dishler A, Kalis J, Kukaine RA, Gren EJ. Expression ofhepatitis B virus surface antigen gene in Escherichia co-li. Gene 1991;30:201-10. 13. Sambrook J, Fritsch EF, Mannatis T. Molecular Cloning:a laboratory manual. Second edition. Cold Spring Har-bor Laboratory Press Printed in the USA, 1989. 14. Buckholz RG, Gleeson MAG. Yeast systems for the com-mercial production of heterologus proteins. Bio/Techno-logy 1991;9:1067-72. 15. Glick BR, Pasternak JJ. Heterologous protein producti-on in eukaryotic cells in chapter 5., Molecular Biotech-nology Washington DC, 1995;113-32. 16. Glick BR, Pasternak JJ. Molecular research proceduresin chapter 3., Molecular Biotechnology Washington DC,1995;75-6.