Journals / Gazi Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 1997 / Cilt: 10 - Sayı: 2

The comparison of ultracentrifugation and miniprep methods for plasmid isolation

Plazmid izolasyonunda ultrasantrifüj ve miniprep tekniklerinin karşılaştırılması

Pages
301–306
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Miniprep procedure has a limitation for further molecular investigations because of this/these extra band(s).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study isolated plasmids were examined using miniprep and ultracentrifugal methods.
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Key Findings & Data:Particularly, an extra band close to 80 kb size seen in miniprep procedure did not appear in ultracentrifugal method.
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Conclusion & Impact:Miniprep procedure has a limitation for further molecular investigations because of this/these extra band(s).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study isolated plasmids were examined using miniprep and ultracentrifugal methods. It was observed that there were some kinds of problems related to miniprep procedure for plasmid isolation from standard bacteria and its known plasmids. Particularly, an extra band close to 80 kb size seen in miniprep procedure did not appear in ultracentrifugal method. Miniprep procedure has a limitation for further molecular investigations because of this/these extra band(s).

Özet

Bu çalışmada miniprep ve ultrasantrifüj yöntemiyle plazmid izolasyonları incelenmiştir. Standart bakteri ve onun bilinen plazmidleriyle yapılan plazmid izolasyonunda miniprep yönteminin çeşitli problemleri olduğu görülmüştür. Özellikle miniprep yönteminde görülen 80 kb civarındaki fazla bant ultrasantrifüj yönteminde görülmemektedir. Miniprep yöntemi içerdiği bu fazla bant veya bantlar ile daha ileri moleküler çalışmalar için sakınca teşkil etmektedir.

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Cite This Article

KUBAR, A., GÜNEY, Ç., AÇIK, L., YAPAR, M., GÜRAN, Ş. (2022). The comparison of ultracentrifugation and miniprep methods for plasmid isolation. Gazi Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 10 - Sayı: 2, 301–306. https://ajindex.com/articles/522649