Journals / İzmir Dr. Behçet Uz Çocuk Hastanesi Dergisi / 2012 / Cilt: 2 - Sayı: 1

Investigation of viral agents by multiplex PCR in children with symptoms of upper respiratory tract infection

Üst solunum yolu infeksiyonlu çocuklarda viral etkenlerin multipleks PCR ile araştırılması

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1–5
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: It was concluded that multiplex PCR is a reliable test which can simultaneously detect numerous viruses in a short time in accordance with the results of this study.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: We evaluate the results of “respiratory viral panel” tested in children with symptoms of acute upper respiratory tract infections.
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Key Findings & Data:Methods: Multiplex PCR test results of nasopharyngeal swap samples of 160 symptomatic children were analyzed.
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Conclusion & Impact:Conclusion: It was concluded that multiplex PCR is a reliable test which can simultaneously detect numerous viruses in a short time in accordance with the results of this study.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: We evaluate the results of “respiratory viral panel” tested in children with symptoms of acute upper respiratory tract infections. Methods: Multiplex PCR test results of nasopharyngeal swap samples of 160 symptomatic children were analyzed. Results: Fifty-five patients (34.4%) had PCR test positivity. Two (n=5 patients; 3.1%), and three (n=1; 0.6%) viral species were detected. Rhinovirus was the most frequently detected species (n= 14; 25.4%). Conclusion: It was concluded that multiplex PCR is a reliable test which can simultaneously detect numerous viruses in a short time in accordance with the results of this study.

Özet

Amaç: Bu çalışmada, akut üst solunum yolu semptomlu çocuklarda test edilmiş “viral solunum paneli” sonuçlarını değerlendirdik. Yöntemler: Toplam 160 semptomlu çocuğun nazofaringeal sürüntü örneklerinin multipleks PCR ile test edilmiş sonuçları incelendi. Bulgular: Hastaların 55 (%34.4)’i pozitifti. Beş hastada (%3.1) çift etken, bir hastada (%0.6) ise üç etken bulundu. En sık olarak insan rinovirüs 14 hastada (%25.4) tespit edildi. Sonuç: Etken viruslarının sıklığının ortaya konulduğu çalışmada, çok sayıda virusu aynı anda ve kısa sürede tespit edebilen multipleks PCR’ın güvenilir bir test olduğu sonucuna varıldı.

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Cite This Article

ECEMİŞ, T., YILMAZ, Ö., ŞANLIDAĞ, T., AKÇALI, S., YÜKSEL, H. (2012). Investigation of viral agents by multiplex PCR in children with symptoms of upper respiratory tract infection. İzmir Dr. Behçet Uz Çocuk Hastanesi Dergisi, Cilt: 2 - Sayı: 1, 1–5. https://ajindex.com/articles/219750

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References (10)

  1. Brittain-Long R, Nord S, Olofsson S, Westin J, Anderson LM, Lindh M. Multiplex real-time PCR for detection of respiratory tract infections. J Clin Virol 2008
  2. 41(1):53-6. http://dx.doi.org/10.1016/j.jcv.2007.10.029 PMid:18093871 2. Bonfim CM, Nogueira ML, Simas PV, Gardinassi LG, Durigon EL, Rahal P, et al. Frequent respiratory pathogens of respiratory tract infections in children attending daycare centers. J Pediatr 2011
  3. 87(5):439-44. http://dx.doi.org/10.2223/JPED.2126 PMid:22125800 3. Coiras MT, Aguilar JC, García ML, Casas I, Pérez-Breña P. Simultaneous Detection of Fourteen Respiratory Viruses in Clinical Specimens by Two Multiplex Reverse Transcription Nested-PCR Assays. J Med Virol 2004
  4. 72(3):484-95. http://dx.doi.org/10.1002/jmv.20008 PMid:14748074 4. Roh KH, Kim J, Nam MH, Yoon S, Lee CK, Lee K, et al. Comparison of the Seeplex Reverse Transcription PCR Assay with the R-mix Viral Culture and Immunofluorescence Techniques for Detection of Eight Respiratory Viruses. Ann Clin Lab Sci 2008
  5. 38(1):41-6. PMid:18316781 5. Syrmis MW, Whiley DM, Thomas M, Mackay IM, Williamson J, Siebert DJ, et al. A Sensitive, Specific, and Cost-Effective Multiplex Reverse Transcriptase-PCR Assay for the Detection of Seven Common Respiratory Viruses in Respiratory Samples. J Mol Diagn 2004
  6. 6(2):125-31. http://dx.doi.org/10.1016/S1525-1578(10)60500-4 6. Mahony J, Chong S, Merante F, Yaghoubian S, Sinha T, Lisle C, et al. Development of a Respiratory Virus Panel Test for Detection of Twenty Human Respiratory Viruses by Use of Multiplex PCR and a Fluid Microbead-Based Assay. J Clin Microbiol 2007
  7. 45(9):2965-70. http://dx.doi.org/10.1128/JCM.02436-06 PMid:17596360 PMCid:2045291 7. Bellau-Pujol S, Vabret A, Legrand L, Dina J, Gouarin S, Petitjean-Lecherbonnier J, et al. Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses. J Virol Methods 2005
  8. 126(1-2):53-63. http://dx.doi.org/10.1016/j.jviromet.2005.01.020 PMid:15847919 8. Hammitt LL, Kazungu S, Welch S, Bett A, Onyango CO, Gunson RN, et al. Added Value of an Oropharyngeal Swab in Detection of Viruses in Children Hospitalized with Lower Respiratory Tract Infection. J Clin Microbiol 2011
  9. 49(6):2318-20. http://dx.doi.org/10.1128/JCM.02605-10 PMid:21490188 PMCid:3122752 9. Tsuchiya LR, Costa LM, Raboni SM, Nogueira MB, Pereira LA, Rotta I, et al. Viral respiratory infection in Curitiba, Southern Brazil. J Infect 2005
  10. http://dx.doi.org/10.1016/j.jinf.2004.12.002 PMid:16321652 10.