Journals / 7tepe Klinik / 2020 / Cilt: 16 - Sayı: 3

Investigation of the effect of composıte materıals on oxidant and antioxidant values ın the gingival fibroblast cells

Kompozit materyallerin gingival fibroblast hücrelerindeki oksidan ve antioksidan değerlerine etkisinin incelenmesi

Pages
237–243
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: In the cytotoxicity of a material; It was concluded that factors such as the structure of the material, the ratio of the monomer it contains, the type of monomer, the filler content are effective as a whole, the increase in the monomer percentages directly affects the antioxidant system, and the particles added to the filler content are also effective in the oxidative stress.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: Direct interactions between oral tissues and dental restorations cause oxidative stress and cellular damage by accumulation of free radicals in the cell.
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Key Findings & Data:Materials and Methods: Six different composite materials were used in the study. (X-tra Fill (Voco-Germany), G- ænial Posterior (GC Tokyo Japan), Estelite Sigma Quick (Tokuyama-Japan), Grandio (Voco-Germany), Arabesque (Voco-Germany) Polofil Supra (Voco-Germany) The number of samples for each material was determined as 12 (n = 12).
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Conclusion & Impact:Conclusion: In the cytotoxicity of a material; It was concluded that factors such as the structure of the material, the ratio of the monomer it contains, the type of monomer, the filler content are effective as a whole, the increase in the monomer percentages directly affects the antioxidant system, and the particles added to the filler content are also effective in the oxidative stress.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: Direct interactions between oral tissues and dental restorations cause oxidative stress and cellular damage by accumulation of free radicals in the cell. Oxidative stress-based measurement methods have an important role in determining biocompatibility of a material. In this study, it was aimed to evaluate the oxidative stress caused by different composite materials in gingival fibroblast cells by TAS (total antioxidant capacity) and TOS (total oxidant capacity) analysis. Materials and Methods: Six different composite materials were used in the study. (X-tra Fill (Voco-Germany), G- ænial Posterior (GC Tokyo Japan), Estelite Sigma Quick (Tokuyama-Japan), Grandio (Voco-Germany), Arabesque (Voco-Germany) Polofil Supra (Voco-Germany) The number of samples for each material was determined as 12 (n = 12). Samples were prepared by using Teflon molds, and oxidative stress status of the cells were evaluated by TAS-TOS (total antioxidant-total oxidant status) analysis as a result of contact of the GFBCs with the samples for 72 hours. Results: TAS values obtained from the groups are as follows; PS> EU> GO> ES> XF> GA; TOS values were determined as GA> XF> ES> GO> AB> PS. Conclusion: In the cytotoxicity of a material; It was concluded that factors such as the structure of the material, the ratio of the monomer it contains, the type of monomer, the filler content are effective as a whole, the increase in the monomer percentages directly affects the antioxidant system, and the particles added to the filler content are also effective in the oxidative stress.

Özet

Amaç: Oral dokular ile dental restorasyonlar arasındaki direkt etkileşimler serbest radikallerin hücrede birikmesi ile oksidatif strese ve hücresel hasara neden olmaktadır. Oksidatif strese dayalı ölçüm yöntemleri bir materyalin biyouyumluluğun belirlenmesinde önemli bir yer edinmektedir. Bu çalışmada farklı kompozit materyallerin gingival fibroblast hücrelerinde meydana getirdiği oksidatif stresin TAS (total antioksidan kapasite) ve TOS (total oksidan kapasite) analizleriyle değerlendirilmesi amaçlandı. Gereç ve Yöntem: Çalışmada 6 yeni nesil kompozit materyal kullanıldı. (X-tra Fill (Voco-Almanya), G- ænial Posterior (GC Tokyo Japonya), Estelite Sigma Quick (Tokuyama-Japonya), Grandio (Voco-Almanya), Arabesk (Voco-Almanya) Polofil Supra (Voco-Almanya) Her materyal için örnek sayısı 12 olarak belirlendi (n=12). Örnekler teflon kalıplar kullanılarak hazırlandı. GFBCs’lerin 72 saat süreyle örneklerle teması sonucu hücrelerde meydana gelen oksidatif stres durumu TAS ve TOS analizleriyle değerlendirildi. Bulgular: Gruplardan elde edilen TAS değerleri sırasıyla; PS>AB>GO>ES>XF>GA olarak; TOS değerleri GA>XF>ES>- GO>AB>PS olarak tespit edildi. Sonuç: Bir materyalin sitotoksisitesinde; materyalin yapısı, içerdiği monomer oranı, monomer tipi, doldurucu içeriği gibi faktörlerin bir bütün olarak etkili olduğu, monomer yüzdele 9rindeki artışın antioksidan sistem üzerine doğrudan etki ettiği, doldurucu içeriğine eklenen parçacıkların da oksidatif streste etkili olduğu sonucuna varıldı.

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Cite This Article

OK, E., TAGHİZADEHGHALEHJOUGHİ, A., KAMALAK, H. (2020). Investigation of the effect of composıte materıals on oxidant and antioxidant values ın the gingival fibroblast cells. 7tepe Klinik, Cilt: 16 - Sayı: 3, 237–243. https://ajindex.com/articles/219363

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References (16)

  1. Hanks CT, Wataha JC, Sun Z. In vitro models of biocompatibility: a review. Dent Mater 1996; 12: 186-193
  2. Schmalz G. The biocompatibility of non-amalgam dental filling materials. Eur J Oral Sci 1998; 106: 696-706
  3. Cramer NB, Stansbury JW, Bowman CN. Recent advances and developments in composite dental restorative materials. J Dent Res 2011; 90: 402-416
  4. Peutzfeldt A. Resin composites in dentistry: the monomer systems. Eur J Oral Sci 1997; 105: 97-116
  5. Van Landuyt KL, Snauwaert J, De Munck J, Peumans M, Yoshida Y, Poitevin A, Coutinho E, Suzuki K, Lambrechts P, Van Meerbeek B. Systematic review of the chemical composition of contemporary dental adhesives. Biomaterials 2007; 28: 3757-3785
  6. St John KR. Biocompatibility of dental materials. Dent Clin North Am 2007; 51: 747-760
  7. Krifka S, Spagnuolo G, Schmalz G, Schweikl H. A review of adaptive mechanisms in cell responses towards oxidative stress caused by dental resin monomers. Biomaterials 2013; 34: 4555-4563
  8. Schweikl H, Spagnuolo G, Schmalz G. Genetic and cellular toxicology of dental resin monomers. J Dent Res 2006; 85: 870-877
  9. Spagnuolo G, Annunziata M, Rengo S. Cytotoxicity and oxidative stress caused by dental adhesive systems cured with halogen and LED lights. Clin Oral Investig 2004; 8: 81-85
  10. Lee DH, Lim BS, Lee YK, Ahn SJ, Yang HC. Involvement of oxidative stress in mutagenicity and apoptosis caused by dental resin monomers in cell cultures. Dent Mater 2006; 22: 1086-1092
  11. Mates JM, Sanchez-Jimenez FM. Role of reactive oxygen species in apoptosis: implications for cancer therapy. Int J Biochem Cell Biol 2000; 32: 157-170
  12. Demirci T GT, Şengül F. Dental Rezin Kompozitlerin Sitotoksisitesi: Bir in Vitro Çalışma. Atatürk Üniv Diş Hek Fak Derg 2014; 24: 10-15
  13. Peumans M, Van Meerbeek B, Lambrechts P, Vanherle G, Quirynen M. The influence of direct composite additions for the correction of tooth form and/or position on periodontal health. A retrospective study. J Periodontol 1998; 69: 422-427
  14. AA KS. Dental döküm alaşımlarının genotoksisite, mutajenitisite ve karsinojenitesini. SÜ Diş Hek Fak Derg 1994; 16: 73-78
  15. Gutteridge JM, Quinlan GJ, Mumby S, Heath A, Evans TW. Primary plasma antioxidants in adult respiratory distress syndrome patients: changes in iron-oxidizing, iron-binding, and free radical-scavenging proteins. J Lab Clin Med 1994; 124: 263-273
  16. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxida