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Journals / Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi / 2021 / Cilt: 31 - Sayı: 1

CYTOTOXICITY OF COMPOSITE MATERIALS ON GINGIVAL FIBROBLAST CELLS (EXPERIMENTAL RESEARCH)

KOMPOZİT MATERYALLERİN GİNGİVAL FİBROBLAST HÜCRELER ÜZERİNDEKİ SİTOTOKSİSİTESİ (DENEYSEL ARAŞTIRMA)

Hakan KAMALAK
Elif OK
Ali TAGHIZADEHGHALEHJOUGHI
Pages
65–70
Publication Date
2022-10-10
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Statistical significance was assessed at p

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: Flow cytometry technique appears to play an important role in determining the viability of cells by passing through a chamber illuminated by laser light.
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Key Findings & Data:In this study, it was aimed to determine the change in cell viability values, apoptotic and necrotic change rates caused by resin-containing composite materials in gingival fibroblast cells by flow cytometry technique.
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Conclusion & Impact:Statistical significance was assessed at p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: Flow cytometry technique appears to play an important role in determining the viability of cells by passing through a chamber illuminated by laser light. In this study, it was aimed to determine the change in cell viability values, apoptotic and necrotic change rates caused by resin-containing composite materials in gingival fibroblast cells by flow cytometry technique. Materials and Methods: Six different composite materials were used in the study. (X-tra Fill (Voco-Germany), G- ænial Posterior (GC Tokyo Japan), Estelite Sigma Quick (Tokuyama-Japan), Grandio (Voco-Germany), Arabesque (Voco-Germany) Polofil Supra (Voco-Germany). The number of samples for each material was 12. (n = 12) Samples were prepared using teflon molds. Cell viability percentages, apoptosis and necrosis percentages were evaluated by flow cytometry analysis of cells in contact with GFBCs during 72 hours. In the analysis of the data, one-way and two-way analysis of variance (ANOVA) method was used to determine the interactions of variables with the control group. Statistical significance was assessed at p

Özet

Amaç: Flow sitometri tekniği süspansiyon halindeki hücrelerin lazer ışığı ile aydınlatılmış olan bir bölmeden geçirilip hücrelerincanlılığının tespit edilmesinde önemli rol oynadığı bilinmektedir. Bu çalışmada rezin içerikli kompozit materyallerin gingivalfibroblast hücrelerinde meydana getirdiği hücre canlılık değerlerindeki, apoptotik ve nekrotik değişim oranlarındaki değişiminflow sitometri tekniği ile tespit edilmesi amaçlandı.Gereç ve Yöntem: Çalışmada 6 farklı yeni nesil kompozit materyali (X-tra Fill (Voco-Almanya), G- ænial Posterior(GC TokyoJaponya), Estelite Sigma Quick(Tokuyama-Japonya) , Grandio (Voco-Almanya), Arabesk (Voco-Almanya) Polofil Supra (VocoAlmanya) kullanıldı. Her materyal için örnek sayısı 12 olarak belirlendi (n=12). Örnekler teflon kalıplar kullanılarak hazırlandı.GFBCs’lerin 72 saat süreyle örneklerle teması sonucu hücrelerde meydana gelen hücre canlılık yüzdeleri, apoptoz ve nekrozyüzdeleri flow sitometre analizleriyle değerlendirildi. Verilerin analizinde, değişkenlerin kontrol grubu ile olan etkileşimlerini tespitetmek için tek yönlü ve iki yönlü varyans analiz (ANOVA) yöntemi kullanıldı. İstatistiksel anlamlılık p

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Cite This Article

KAMALAK, H., OK, E., TAGHIZADEHGHALEHJOUGHI, A. (2022). CYTOTOXICITY OF COMPOSITE MATERIALS ON GINGIVAL FIBROBLAST CELLS (EXPERIMENTAL RESEARCH). Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, Cilt: 31 - Sayı: 1, 65–70. https://ajindex.com/articles/451523

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