SavedAdd your journal

Journals / Dicle Tıp Dergisi / 2017 / Cilt: 44 - Sayı: 3

Histopathological effects of Platelet rich fibrin on peripheral nerve healing

Trombositten zengin Fibrinin periferik sinir iyileşmesi üzerindeki histopatolojik etkileri

Hasan METİNEREN
Turan Cihan DÜLGEROĞLU
Mehmet Hüseyin METİNEREN
Pages
243–250
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: PRF, which contains abundant amounts of cellular content and growth factors, provides a positive contribution to nerve healing.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objectives: Experimental studies have been carried out with various materials in order to accelerate and improve the quality of nerve healing.
📊
Key Findings & Data:Methods:16 Sprague Dawley rats were randomly divided into the two groups (8 controls, 8 PRF).
💡
Conclusion & Impact:Conclusion: PRF, which contains abundant amounts of cellular content and growth factors, provides a positive contribution to nerve healing.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: Experimental studies have been carried out with various materials in order to accelerate and improve the quality of nerve healing. Purpose of our study to investigate the histopathological effects of platelet rich fibrin (PRF) on sciatic nerve healing in an experimental rat model. Methods:16 Sprague Dawley rats were randomly divided into the two groups (8 controls, 8 PRF). The sciatic nerves of all rats were transected and repaired with a 2 mm defect. In the PRF group, the defective zone was surrounded with PRF. Both group rats were sacrified and histopathologically examined at the end of 6 weeks. Result: PRF group histological scores were statistically significant (P = 0.001) compared to the control group. Axonal continuity was present in the PRF group nerves. In the control group, improvement of the defect with granulation texture was observed. Conclusion: PRF, which contains abundant amounts of cellular content and growth factors, provides a positive contribution to nerve healing.

Özet

Amaç: Sinir iyileşmesini hızlandırmak ve kalitesini artırmak için çeşitli materyallerle deneysel çalışmalar yapılmıştır. Çalışmamızın amacı trombositten zengin fibrinin (TZF) siyatik sinir iyileşmesi üzerindeki histopatolojik etkilerini deneysel rat modelinde araştırmaktır. Yöntemler: 16 Sprague Dawley rat randomize iki guruba (8 kontrol, 8 TZF) ayrıldı. Tüm ratların siyatik sinirleri kesilip 2mm defektli olarak tekrar onarıldı. TZF gurubunda defektif bölgeye TZF sarıldı. 6 haftanın sonunda ratlar sakrifiye edilerek histolojik ve immüno-histokimyasal metotlarla TZF nin kontrol gurubuna göre sinir onarımına katkısı araştırıldı. Sonuç: TZF gurubunun histolojik skorları, kontrol gurubuna göre istatistiksel olarak anlamlı bulundu(P=0.001). TZF sinirlerde aksonal devamlılık mevcuttu. Kontrol grubunda defektin tamamen iyileşmediği yada fibröz/granülasyon dokuyla iyileştiği gözlendi. Bol miktarda hücresel içerik ve büyüme faktörü ihtiva eden TZF sinir iyileşmesine olumlu katkı sağlamaktadır.

🔖

Cite This Article

METİNEREN, H., DÜLGEROĞLU, T. C., METİNEREN, M. H. (2017). Histopathological effects of Platelet rich fibrin on peripheral nerve healing. Dicle Tıp Dergisi, Cilt: 44 - Sayı: 3, 243–250. https://ajindex.com/articles/199081

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Noble J, Munro CA, Prasad VS, et al. Analysis of upper and lower extremity peripheral nerve injuries in a population of patients with multiple injuries. J Trauma 1998; 45:116-22
  2. Choukroun J, Diss A, Simonpieri A, et al. Platelet-rich fibrin (PRF): a second-generation platelet. s.l.: concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 101:299-303
  3. Weibrich G, Kleis WKG, Hafner G. Growth factor levels in platelet rich plasma and correlation with donor age, sex and platelet count. J Craniomaxillofac Surg 2002; 30:97-102
  4. Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates from pure platelet-rich plasma (P-PRP) to leucocyte and plateletrich fibrin (L-PRF). Trends in Biotechnology 2009; 27:158-67
  5. Sean M. O'Connell, Theresa Impeduglia, Karen Hessler, et al. Autologous platelet-rich fibrin matrix as cell therapy in the healing of chronic lower-extremity ulcers. Wound Repair and Regeneration 2008;16:749- 56
  6. Klopfleisch R. Multiparametric and semiquantitative scoring systems for the evaluation of mouse model histopathology--a systematic review. BMC Vet Res 2013; 9:123
  7. Giddins GE, Wade PJ, Amis AA. Primary nerve repair: strength of repair with different gauges of nylon suture material. J Hand Surg Br. 1989; 14:301-2
  8. Panagopoulos GN, Megaloikonomos PD, Mavrogenis AF. The Present and Future for Peripheral Nerve Regeneration. Orthopedics. 2016; 25:1-16
  9. Sariguney Y, Yavuzer R, Elmas C. Effect of platelet-rich plasma on peripheral nerve regeneration. J Reconstr Microsurg 2008;24:159-67
  10. Piskin A, Kaplan S, Aktas A, et al. Platelet gel does not improve peripheral nerve regeneration: An electrophysiological, stereological, and electron microscopic study. Microsurgery 2009; 29:144-53
  11. Şenses F, Önder ME, Koçyiğit ID, et al. Effect of Platelet-Rich Fibrin on Peripheral Nerve Regeneration. J Craniofac Surg. 2016;27: 1759-64
  12. Lichtenfels M, Colomé L, Sebben AD, et al. Effect of Platelet Rich Plasma and Platelet Rich Fibrin on sciatic nerve regeneration in a rat model. Microsurgery 2013; 33: 383-90
  13. Giannessi E, Coli A, Stornelli MR, et al. An autologously generated platelet-rich plasma suturable membrane may enhance peripheral nerve regeneration after neurorraphy in an acute injury model of sciatic nerve neurotmesis. J Reconstr Microsurg. 2014; 30: 617-26
  14. Uysal AC, Mizuno H. Tendon re