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Journals / Tarım Bilimleri Dergisi / 2014 / Cilt: 20 - Sayı: 4

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Türkiye’de Yetiştirilen Bazı Yerli Koyun Irklarında Kalpastatin (CAST) Geni Polimorfizminin PCR-RFLP Yöntemiyle Belirlenmesi

Murat BALCIOĞLU
Taki KARSLI
Emine ŞAHİN
Zafer ULUTAŞ
Yüksel AKSOY
Pages
427–433
Publication Date
2014-10-16
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to chi-square test, all the other populations were consistent with Hardy-Weinberg equilibrium, whereas Morkaraman, İvesi and Karayaka populations showed significant (P

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, calpastatin (CAST) gene polimorphism was investigated in 7 native sheep breeds reared in Turkey by using PCR-RFLP method.
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Key Findings & Data:The frequencies of M and N alleles of CAST gene in Kangal (n= 31), Awassi (n= 26), Güney Karaman (n= 23), Akkaraman (n= 21), Morkaraman (n= 34), Karayaka (n= 33), and Karakas (n= 22) sheep breeds were determined as 0.92-0.08, 0.59-0.41, 0.67-0.33, 0.69-0.31, 0.87-0.13, 0.86-0.14, 0.89-0.11 respectively.
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Conclusion & Impact:According to chi-square test, all the other populations were consistent with Hardy-Weinberg equilibrium, whereas Morkaraman, İvesi and Karayaka populations showed significant (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Kalpastatin geni · Polimorfzim · PCR-RFLP

Abstract

In this study, calpastatin (CAST) gene polimorphism was investigated in 7 native sheep breeds reared in Turkey by using PCR-RFLP method. The frequencies of M and N alleles of CAST gene in Kangal (n= 31), Awassi (n= 26), Güney Karaman (n= 23), Akkaraman (n= 21), Morkaraman (n= 34), Karayaka (n= 33), and Karakas (n= 22) sheep breeds were determined as 0.92-0.08, 0.59-0.41, 0.67-0.33, 0.69-0.31, 0.87-0.13, 0.86-0.14, 0.89-0.11 respectively. According to chi-square test, all the other populations were consistent with Hardy-Weinberg equilibrium, whereas Morkaraman, İvesi and Karayaka populations showed significant (P

Özet

Bu çalışmada, Türkiye’de yetiştirilen 7 yerli koyun ırkında kalpastatin (CAST) gen polimorfizmi PCR-RFLP metodu kullanılarak araştırılmıştır. CAST geninin M ve N allelleri frekansları; Kangal (n= 31), İvesi (n= 26), Güney Karaman(n= 23), Akkaraman (n= 21), Morkaraman (n= 34), Karayaka (n= 33) ve Karakaş (n= 22) koyun ırklarında sırasıyla 0.92-0.08, 0.59-0.41, 0.67-0.33, 0.69-0.31, 0.87-0.13, 0.86-0.14, 0.89-0.11 olarak tespit edilmiştir. Ki-kare testi CAST geni bakımından Morkaraman, İvesi ve Karayaka populasyonlarının Hardy-Weinberg dengesinden önemli düzeyde (P<0.05)saptığını, diğer populasyonların ise Hardy-Weinberg dengesinde olduğunu göstermiştir.

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Cite This Article

BALCIOĞLU, M., KARSLI, T., ŞAHİN, E., ULUTAŞ, Z., AKSOY, Y. (2014). -. Tarım Bilimleri Dergisi, Cilt: 20 - Sayı: 4, 427–433. https://ajindex.com/articles/178514

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