Journals / Hayvansal Üretim / 2020 / Cilt: 61 - Sayı: 2

Comparing Autosomal SSR and PCR-RFLP Markers to Determine Phylogenetic Relationship Based on Genetic Distances in Livestock

Çiftlik Hayvanlarında Genetik Mesafe Temelli Filogenetik İlişkinin Belirlenmesinde Otozomal SSR ve PCR-RFLP Markerlerinin Karşılaştırılması

Pages
135–141
Publication Date
2020-12-29
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this context, in the present study, Simple Sequence Repeats and Polymerase Chain Reaction- Restriction Fragment Length Polymorphism techniques were compared to reveal phylogenetic relationship based on genetic distances.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Many molecular tools are available to analyse phylogenetic relationships in livestock.
📊
Key Findings & Data:Nowadays, Simple Sequence Repeats and Single Nucleotide Polymorphisms are commonly used molecular techniques to determine phylogenetic relationships in livestock breeds or types.
💡
Conclusion & Impact:In this context, in the present study, Simple Sequence Repeats and Polymerase Chain Reaction- Restriction Fragment Length Polymorphism techniques were compared to reveal phylogenetic relationship based on genetic distances.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Çiftlik hayvanları · UPGMA dendogramı · moleküler markerler

Abstract

Objective: Many molecular tools are available to analyse phylogenetic relationships in livestock. Nowadays, Simple Sequence Repeats and Single Nucleotide Polymorphisms are commonly used molecular techniques to determine phylogenetic relationships in livestock breeds or types. However, alternative molecular techniques may be preferred to conduct phylogenetic analysis in case of limiting conditions such as budget and time. In this context, in the present study, Simple Sequence Repeats and Polymerase Chain Reaction- Restriction Fragment Length Polymorphism techniques were compared to reveal phylogenetic relationship based on genetic distances.

Özet

Amaç: Çiftlik hayvanlarında filogenetik ilişkinin analizi için çok sayıda moleküler araç nmaktadır. Günümüzde çiftlik hayvanı ırk ve tiplerinde filogenetik ilişkinin belirlenmesinde Basit Dizi Tekrarları ve Tek Nükleotid Polimorfizmleri en yaygın kullanılan moleküler tekniklerdir. Bununla birlikte, bütçe ve zaman gibi sınırlayıcı koşullarda filogenetik analiz yapabilmek için alternatif moleküler teknikler tercih edilebilir. Bu bağlamda, mevcut çalışmada genetik mesafe temelli filogenetik ilişkinin ortaya çıkarılmasında Basit Dizi Tekrarları ve Polimeraz Zincir ReaksiyonuRestriksiyon Parça Uzunluk Polimorfizmi teknikleri karşılaştırılmıştır.

🔖

Cite This Article

KARSLI, T., DEMİR, E., KARSLI, B. A., FİDAN, H. G., BALCIOĞLU, M. (2020). Comparing Autosomal SSR and PCR-RFLP Markers to Determine Phylogenetic Relationship Based on Genetic Distances in Livestock. Hayvansal Üretim, Cilt: 61 - Sayı: 2, 135–141. https://ajindex.com/articles/86562

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (9)

  1. Al-Araimi NA, Gaafar OM, Costa V, Neira AL, Al-Atiyat RM, Beja-Pereira A. 2017. Genetic origin of goat populations in Oman revealed by mitochondrial DNA analysis. Plos One 12(12): e0190235. Anila H, Paolo AM, Gentian H, Consortıum E. 2010. Genetic diversity in albanian sheep breeds estimated by AFLP markers. Albanian Journal of Agricultural Sciences 9(2): 23-29. Balcıoğlu MS, Șahin E, Karabağ K, Karslı T, Alkan S. 2010. Determination of DNA fingerprinting of Turkish fat-tailed sheep breeds by RAPD-PCR method. Tarim Bilimleri Dergisi 16(1): 55-61.
  2. Elmacı C, Öner Y, Koyuncu M. Saanen Keçilerinde b-laktoglobulin Genotiplerinin PCR-RFLP Yöntemi ile Belirlenmesi. Hayvansal Üretim 49(1): 1-4. FAO. 2007. The state of the world's animal genetic resources for food and agriculture – in brief, edited by Dafydd Pilling & Barbara Rischkowsky. Rome. Ganbold O, Lee SH, Seo D, Paek WK, Manjula P, Munkhbayar M, Lee JH. 2019. Genetic diversity and the origin of Mongolian native sheep. Livestock science 220: 17-25.
  3. Göğer H, Demirtaş ŞE, Yurtoğullari Ş, Taşdemir AN, Şenkal UE, Boyali B. 2017. Breeding studies on pure lines at Poultry Research Institute. Tavukçuluk Araştırma Dergisi 14 (2): 30-38.
  4. Groeneveld LF, Lenstra JA, Eding H, Toro MA, Scherf B, Pilling D, Negrini R, Finlay EK, Jianlin H, Groeneveld E, Weigend S, Consortium TG. 2010. Genetic diversity in farm animals–a review. Animal genetics 4(1): 6-31.
  5. Hailu A, Getu A. 2015. Breed characterization: Tools and their applications. Open Access Library Journal 2(4): e1438 Kanginakudru S, Metta M, Jakati RD, Nagaraju J. 2008. Genetic evidence from Indian red jungle fowl corroborates multiple domestication of modern day chicken. BMC Evolution Biology 8(174): 1-14.
  6. Karslı T, Balcıoğlu MS, Demir E, Fidan HG, Aslan M, Aktan S, Kamanlı S, Karabağ K, Şahin E. 2017. Determination of polymorphisms in IGF-I and NPY candidate genes associated with egg yield in pure layers chicken lines reared in the Ankara Poultry Research Institute. Turkish Journal of Agriculture-Food Science and Technology 5(9): 1051-1056.
  7. Karslı T, Balcıoğlu MS. 2019. Genetic characterization and population structure of six brown layer pure lines using microsatellite markers. Asian-Australasian Journal of Animal Sciences 32(1): 49-57.
  8. Karslı T, Fidan HG. 2019. Assessment of genetic diversity and conservation priorities among five White Leghorn Lines based on SSR markers. Animal Science Papers & Reports, 37(3): 311-322.
  9. Liu YP, Wu GS, Yao YG, Miao YW, Luikart G, Bai