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Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2010 / Cilt: 16 - Sayı: 6

An investigation of presence of FecB allele on BMPR-IB (Booroola) gene raised in Turkey in six local sheep breeds using PCR-RFLP method

Türkiye’de yetiştirilen altı yerli koyun ırkında BMPR-IB (Booroola) geninde FecB allel varlığının PCR-RFLP yöntemiyle araştırılması

TAKİ KARSLI
MURAT SONER BALCIOĞLU
Pages
1033–1036
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result of these analysis, FecB allele couldn’t be determined in totally 111 samples obtained from Akkaraman (19 samples), Morkaraman (19 samples), Dağlıç (18 samples), Ivesi (18 samples), Tuj (18 samples) and Karakaş (19 samples), which are the sheeps randomly selected from the flocks which are reared in different regions of Turkey.

📌 3 Key Takeaways

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Objective & Hypothesis:The presence of FecB allele on Booroola major gene (BMPR-IB) which has an significant effect on the prolificacy was investigated in six local sheep breeds reared in Turkey, by using PCR-RFLP method.
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Key Findings & Data:The presence of FecB allele on Booroola major gene (BMPR-IB) which has an significant effect on the prolificacy was investigated in six local sheep breeds reared in Turkey, by using PCR-RFLP method.
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Conclusion & Impact:As a result of these analysis, FecB allele couldn’t be determined in totally 111 samples obtained from Akkaraman (19 samples), Morkaraman (19 samples), Dağlıç (18 samples), Ivesi (18 samples), Tuj (18 samples) and Karakaş (19 samples), which are the sheeps randomly selected from the flocks which are reared in different regions of Turkey.
🔬Design:Research Article
📍Scope:111 samples
⚙️Variables:analitik yöntemler · teknikler · koyun ırkları

Abstract

The presence of FecB allele on Booroola major gene (BMPR-IB) which has an significant effect on the prolificacy was investigated in six local sheep breeds reared in Turkey, by using PCR-RFLP method. As a result of these analysis, FecB allele couldn’t be determined in totally 111 samples obtained from Akkaraman (19 samples), Morkaraman (19 samples), Dağlıç (18 samples), Ivesi (18 samples), Tuj (18 samples) and Karakaş (19 samples), which are the sheeps randomly selected from the flocks which are reared in different regions of Turkey.

Özet

Bu çalışmada, Türkiye’de yetiştirilen altı yerli koyun ırkında, çoklu doğumu arttıran Booroola (BMPR-IB) majör geninde FecB allelinin varlığı PCR-RFLP metodu kullanılarak araştırılmıştır. Yapılan analizler sonucunda Türkiye’nin değişik bölgelerinde yetiştirilen sürülerden rastgele seçilen Akkaraman (19 örnek), Morkaraman (19 örnek), Dağlıç (18 örnek), İvesi (18 örnek), Tuj (18 örnek) ve Karakaş (19 örnek) koyunlarına ait toplam 111 örnekte FecB alleli tespit edilememiştir.

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Cite This Article

KARSLI, T., BALCIOĞLU, M. S. (2010). An investigation of presence of FecB allele on BMPR-IB (Booroola) gene raised in Turkey in six local sheep breeds using PCR-RFLP method. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 16 - Sayı: 6, 1033–1036. https://ajindex.com/articles/273816

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References (12)

  1. Laçin E, Aksoy AR: Kars bölgesinde yetiştirilen Morkaraman ve Tuj kuzularının büyüme özelliklerinin karşılaştırılması. Kafkas Univ Vet Fak Derg, 9 (1): 33-37, 2003
  2. Karaca O, Cemal İ, Atay O: Hayvancılıkta kimi majör genlerin aktarımı ve kullanımı olanakları. Ulusal Hayvancılık Kongresi, İzmir, 728-732, 18-20 Eylül, 1996
  3. Morris CA: Theoretical and realised responses to selection for reproductive rate. Proc of the 4th World Congress on Genetics Applied to Livestock Production, Edinburgh, 16, 309-318, July, 23-27, 1990
  4. Montogomery GW, Mcnatty KP, Davis GH: Physiology and molecular genetics of mutations that increase ovulation rate in sheep. Endocr Rev, 13 (2): 309-328, 1992
  5. Cemal İ: Çiftlik hayvanlarında majör genler, bunların belirlenmesi, transferi ve endüstriyel kullanımı. Yüksek lisans tezi. Yüzüncü Yıl Üniv Fen Bil Enst, Van, 1996
  6. Davis GH: Fecundity genes in sheep. Anim Reprod Sci, 82- 83, 247-253, 2004
  7. Wilson T, Wu XY, Juengel JL, Ross IK, Lumsden M, Lord EA, Dodds KG, Walling GA, McEwan JC, O’connel AR, Mcnatty KP, Montogomery GW: Highly prolific Booroola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB receptor (ALK-6) that is expressed in both oocytes and granulosa cells. Biol Reprod, 64, 1225-1235, 2001
  8. Souza CJH, Macdougall C, Campbell BK, Mcneilly AS, Baird DT: The booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type IB (BMPRIB) gene. J Endocrinol, 169 (2): 1-6, 2001
  9. Mulsant P, Lecerf F, Fabre S, Schibler L, Monget P, Lenneluc I, Pisselet C, Riquet J, Monniaux D, Calebaut I, Cribıu E, Thimonieri J, Teyssieri J, Bodin L, Cognie Y, Chitour N, Elsen JM: Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Merino ewes. Proc Natl Acad Sci, 98, 5104-5109, 2001
  10. Kumar S, Kolte AP, Mishra AK, Arora AL, Singh VK: Identification of the FecB mutation in Garole Malpura sheep and its effect on litter size. Small Rumin Res, 64, 305-310, 2006
  11. Hanrahan JP, Gregan MS, Mulsant P, Mullen M, Davis GH, Powel R, Galloway SM: Mutation in the genes for oocyte derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries). Biol Reprod, 70, 900-909, 2004
  12. Davis GH, Balakrishnan L, Ross IK, Wilson T, Galloway SM, Lumsden JM, Hanrahan JP, Mullen M, Mao XZ, Wang GL, Zhao ZS, Zeng YQ, Robinson JJ, Mayrogenis AP