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Effects of Modifying the Seed Microbiome via Bacterial Inoculation During the Flowering Stage and Direct Bacterial Seed Coating Against Fusarium Crown and Root Rot in Tomato and Eggplant Seedlings

Çiçeklenme Döneminde Bakteriyel İnokülasyon Yoluyla Tohum Mikrobiyomunun Modifiye Edilmesinin ve Doğrudan Bakteriyel Tohum Kaplamanın Domates ve Patlıcan Fidelerinde Fusarium Kök ve Kökboğazı Çürüklüğüne Etkileri

Zühtü Polat(SAKARYA UYGULAMALI BİLİMLER ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ)0000-0002-4630-6940
Didem Canik Orel(ANKARA ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ)0000-0001-8140-379X
Kutay Coşkun Yıldırım(Atatürk Bahçe Kültürleri Merkez Araştırma Enstitüsü Müdürlüğü)0000-0002-5762-8128
Journal
Bahçe
Pages
—
Publication Date
2026-10-23
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Overall, flowering-stage inoculation produced seeds with biologically meaningful protective capacity, but direct bacterial seed coating provided more consistent protection under severe FORL pressure.

📌 3 Key Takeaways

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Objective & Hypothesis:Fusarium crown and root rot, caused by Fusarium oxysporum f. sp. radicis-lycopersici (FORL), is a Fusarium crown and root rot, caused by Fusarium oxysporum f. sp. radicis-lycopersici (FORL), is a destructive soil-borne disease of tomato and other solanaceous crops.
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Key Findings & Data:Seeds from parent plants inoculated at flowering with Pseudomonas fluorescens L5b or Pseudomonas gessardii L13 (L5-B, L13) were compared with directly coated seeds (K-L5-B, KL-13).
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Conclusion & Impact:Overall, flowering-stage inoculation produced seeds with biologically meaningful protective capacity, but direct bacterial seed coating provided more consistent protection under severe FORL pressure.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:biyolojik mücadele · endofitik bakteriler · Fusarium oxysporum f. sp. radicis-lycopersici

Abstract

Fusarium crown and root rot, caused by Fusarium oxysporum f. sp. radicis-lycopersici (FORL), is a Fusarium crown and root rot, caused by Fusarium oxysporum f. sp. radicis-lycopersici (FORL), is a destructive soil-borne disease of tomato and other solanaceous crops. This two-year study evaluated whether seed microbiome modification via flowering-stage inoculation with endophytic bacteria could protect tomato and eggplant seedlings against FORL, compared with conventional direct seed coating. Seeds from parent plants inoculated at flowering with Pseudomonas fluorescens L5b or Pseudomonas gessardii L13 (L5-B, L13) were compared with directly coated seeds (K-L5-B, KL-13). Seedlings were challenged with FORL and assessed six weeks later. In eggplant, K-L5-B achieved the greatest disease reduction in both years (19.44% and 24.56%), while L13 failed to suppress disease in year one. In tomato, K-L5-B reduced disease severity from 64.88% (positive control) to 46.40% (28.48% reduction), followed by KL-13 (24.49%), L13 (20.83%), and L5-B (18.02%). Bacterial treatments partially mitigated FORL-induced growth impairment, especially for chlorophyll content and shoot fresh weight. In eggplant, KL-13 increased shoot fresh weight to 27.15 g, exceeding the positive control (22.13 g) and approaching the healthy control (26.19 g). Principal component analysis explained 84.3% of growth variation, with biomass traits driving treatment separation. Overall, flowering-stage inoculation produced seeds with biologically meaningful protective capacity, but direct bacterial seed coating provided more consistent protection under severe FORL pressure.

Özet

Fusarium kök ve kökboğazı çürüklüğü (Fusarium oxysporum f. sp. radicis-lycopersici – FORL), domates ve diğer solanaceae türlerinde yıkıcı kayıplara yol açan toprak kökenli bir hastalıktır. Bu iki yıllık çalışmada, çiçeklenme döneminde endofitik bakteri inokülasyonu yoluyla tohum mikrobiyomunun değiştirilmesinin domates ve patlıcan fidelerini FORL'a karşı koruyup koruyamayacağı, geleneksel doğrudan tohum kaplama yöntemiyle karşılaştırılarak değerlendirilmiştir. Çiçeklenmede Pseudomonas fluorescens L5b veya Pseudomonas gessardii L13 ile inoküle edilen ana bitkilerden elde edilen tohumlar (L5-B, L13), aynı suşlarla doğrudan kaplanan tohumlarla (K-L5-B, KL-13) karşılaştırılmıştır. Fideler FORL ile inoküle edilmiş ve altı hafta sonra değerlendirilmiştir. Patlıcanda K-L5-B her iki yılda da en yüksek hastalık azalımını sağlarken (%19,44 ve %24,56), L13 ilk yıl hastalığı baskılayamamıştır. Domateste K-L5-B hastalık şiddetini pozitif kontrolde %64,88'ten %46,40'a düşürmüş (%28,48 azalım), bunu KL-13 (%24,49), L13 (%20,83) ve L5-B (%18,02) izlemiştir. Bakteri uygulamaları FORL kaynaklı gelişme geriliğini kısmen hafifletmiş, özellikle klorofil SPAD değeri ve gövde yaş ağırlığında iyileşme sağlamıştır. Patlıcanda KL-13 gövde yaş ağırlığını 27,15 g'a yükselterek pozitif kontrolü (22,13 g) aşmış ve sağlıklı kontrole (26,19 g) yaklaşmıştır. Temel bileşen analizi, büyüme varyasyonunun %84,3'ünü açıklamış ve uygulamalar arası ayrımda biyokütle özelliklerinin belirleyici olduğunu göstermiştir. Genel olarak, çiçeklenme dönemi inokülasyonu biyolojik açıdan anlamlı koruma sağlayan tohumlar oluşturmuş; ancak şiddetli FORL baskısı altında en tutarlı koruma doğrudan bakteriyel tohum kaplama ile elde edilmiştir.

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Cite This Article

Polat, Z., Orel, D. C., Yıldırım, K. C. (2026). Effects of Modifying the Seed Microbiome via Bacterial Inoculation During the Flowering Stage and Direct Bacterial Seed Coating Against Fusarium Crown and Root Rot in Tomato and Eggplant Seedlings. Bahçe, Cilt: 55 Sayı: 2. https://doi.org/10.53471/bahce.1976658

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References (36)

  1. Abdallah, R. A. B., Jabnoun-Khiareddine, H., Nefzi, A., Ayed, F., & Daami-Remadi, M. (2019). Field suppression of Fusarium wilt and microbial population shifts in tomato rhizosphere following soil treatment with two selected endophytic bacteria. Eurasian Journal of Soil Science, 8(3), 208–220. https://doi.org/10.18393/ejss.556780
  2. Alabouvette, C., Lemanceau, P., & Steinberg, C. (1993). Recent advances in the biological control of Fusarium wilts. Pesticide Science, 37(4), 365–373. https://doi.org/10.1002/ps.2780370409
  3. Altınok, H. H., Can, C., Dikilitaş, M., & Çolak, H. (2012). Türkiye’de açık tarla patlıcan yetiştiriciliğinde Fusarium solgunluk hastalığının yaygınlığı ve izolatların virülenslikleri. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 16(3), 342–352.
  4. Ateş, C., Fidan, H., Karacaoğlu, M., & Daşgan, H. (2019). The identification of the resistance levels of Fusarium oxysporum f. sp. radicis-lycopersici and Tomato yellow leaf curl viruses in different tomato genotypes with traditional and molecular methods. Applied Ecology and Environmental Research, 17(2), 2203–2218. https://doi.org/10.15666/aeer/1702_22032218
  5. Bergna, A., Cernava, T., Rändler, M., Grosch, R., Zachow, C., & Berg, G. (2018). Tomato seeds preferably transmit plant beneficial endophytes. Phytobiomes Journal, 2(4), 183–193. https://doi.org/10.1094/PBIOMES-06-18-0029-R
  6. Bolwerk, A., Lagopodi, A. L., Wijfjes, A. H. M., Lamers, G. E. M., Chin-A-Woeng, T. F. C., Lugtenberg, B. J. J., & Bloemberg, G. V. (2003). Interactions in the tomato rhizosphere of two Pseudomonas biocontrol strains with the phytopathogenic fungus Fusarium oxysporum f. sp. radicis-lycopersici. Molecular Plant-Microbe Interactions, 16(11), 983–993. https://doi.org/10.1094/MPMI.2003.16.11.983
  7. Cabral, C., Gonçalves, A., Fonseca, M., Urben, A., Costa, H., Lourenço, V., Boiteux, L., & Reis, A. (2020). First detection of Fusarium oxysporum f. sp. radicis-lycopersici across major tomato-producing regions in Brazil. Phytoparasitica, 48, 545–553. https://doi.org/10.1007/s12600-020-00824-5
  8. Can, C., Yucel, S., Korolev, N., & Katan, T. (2004). First report of Fusarium crown and root rot of tomato caused by Fusarium oxysporum f. sp. radicis-lycopersici in Turkey. Plant Pathology, 53, 814. https://doi.org/10.1111/j.1365-3059.2004.01087.x
  9. Chin-A-Woeng, T. F. C., Bloemberg, G. V., van der Bij, A. J., van der Drift, K. M. G. M., Schripsema, J., Kroon, B., Scheffer, R. J., Keel, C., Bakker, P. A. H. M., Tichy, H. V., de Bruijn, F. J., Thomas-Oates, J. E., & Lugtenberg, B. J. J. (1998). Biocontrol by phenazine-1-carboxamide-producing Pseudomonas chlororaphis PCL1391 of tomato root rot caused by Fusarium oxysporum f. sp. radicis-lycopersici. Molecular Plant-Microbe Interactions, 11(11), 1069–1077. https://doi.org/10.1094/MPMI.1998.11.11.1069
  10. Chin-A-Woeng, T. F. C., Bloemberg, G. V., Mulders, I. H. M., Dekkers, L. C., & Lugtenberg, B. J. J. (2000). Root colonization by phenazine-1-carboxamide-producing bacterium Pseudomonas chlororaphis PCL1391 is essential for biocontrol of tomato foot and root rot. Molecular Plant-Microbe Interactions, 13(12), 1340–1345. https://doi.org/10.1094/MPMI.2000.13.12.1340
  11. Çakır, B., Gül, A., Yolageldi, L., & Özaktan, H. (2014). Response to Fusarium oxysporum f. sp. radicis-lycopersici in tomato roots involves regulation of SA- and ET-responsive gene expressions. European Journal of Plant Pathology, 139, 379–391. https://doi.org/10.1007/s10658-014-0394-9
  12. Çolak, A., & Biçici, M. (2011). Doğu Akdeniz Bölgesi örtü altı domates yetiştiriciliğinde Fusarium oxysporum spesiyal formlarının simptomatolojik ayrımı ile solgunluk ve kök-kök boğazı çürüklüğü hastalıklarının çıkış, şiddet ve yaygınlıklarının belirlenmesi. Bitki Koruma Bülteni, 51(4), 331–345.
  13. de Weert, S., Kuiper, I., Lagendijk, E. L., Lamers, G. E. M., & Lugtenberg, B. J. J. (2004). Role of chemotaxis toward fusaric acid in colonization of hyphae of Fusarium oxysporum f. sp. radicis-lycopersici by Pseudomonas fluorescens WCS365. Molecular Plant-Microbe Interactions, 17(11), 1185–1191. https://doi.org/10.1094/MPMI.2004.17.11.1185
  14. Kamilova, F., Kravchenko, L. V., Shaposhnikov, A. I., Azarova, T., Makarova, N., & Lugtenberg, B. (2006). Effects of the tomato pathogen Fusarium oxysporum f. sp. radicis-lycopersici and of the biocontrol bacterium Pseudomonas fluorescens WCS365 on the composition of organic acids and sugars in tomato root exudate. Molecular Plant-Microbe Interactions, 19(10), 1121–1126. https://doi.org/10.1094/MPMI-19-1121
  15. Kamilova, F., Lamers, G., & Lugtenberg, B. (2008). Biocontrol strain Pseudomonas fluorescens WCS365 inhibits germination of Fusarium oxysporum spores in tomato root exudate as well as subsequent formation of new spores. Environmental Microbiology, 10(9), 2455–2461. https://doi.org/10.1111/j.1462-2920.2008.01638.x Kamilova, F., Validov, S., Azarova, T., Mulders, I., & Lugtenberg, B. (2005). Enrichment for enhanced competitive plant root tip colonizers selects for a new class of biocontrol bacteria. Environmental Microbiology, 7(11), 1809–1817. https://doi.org/10.1111/j.1462-2920.2005.00889.x
  16. Kamou, N. N., Karasali, H., Menexes, G., Kasiotis, K. M., Bon, M. C., Papadakis, E. N., Tzelepis, G. D., & Lagopodi, A. L. (2015). Isolation, screening and characterisation of local beneficial rhizobacteria based upon their ability to suppress Fusarium oxysporum f. sp. radicis-lycopersici and tomato foot and root rot. Biocontrol Science and Technology, 25(8), 928–949. https://doi.org/10.1080/09583157.2015.1020762
  17. King, E. O., Ward, M. K., & Raney, D. E. (1954). Two simple media for the demonstration of pyocyanin and fluorescin. Journal of Laboratory and Clinical Medicine, 44(2), 301–307.
  18. Manzo, D., Ferriello, F., Puopolo, G., Zoina, A., D’Esposito, D., Tardella, L., Ferrarini, A., & Ercolano, M. R. (2016). Fusarium oxysporum f. sp. radicis-lycopersici induces distinct transcriptome reprogramming in resistant and susceptible isogenic tomato lines. BMC Plant Biology, 16, Article 53. https://doi.org/10.1186/s12870-016-0740-5 McGovern, R. J. (2015). Management of tomato diseases caused by Fusarium oxysporum. Crop Protection, 73, 78–92. https://doi.org/10.1016/j.cropro.2015.02.021
  19. Mitter, B., Pfaffenbichler, N., Flavell, R., Compant, S., Antonielli, L., Petric, A., Berninger, T., Naveed, M., Sheibani-Tezerji, R., von Maltzahn, G., & Sessitsch, A. (2017). A new approach to modify plant microbiomes and traits by introducing beneficial bacteria at flowering into progeny seeds. Frontiers in Microbiology, 8, Article 11. https://doi.org/10.3389/fmicb.2017.00011
  20. Mohamed, D., Gaston, K., Guy, B., Carine, N., Aicha, K., & Daouda, K. (2021). Efficacy of four essential oils on Fusarium head blight caused by Fusarium oxysporum f. sp. radicis-lycopersici, a fungal telluric pathogen of tomato grown under cover. International Journal of Advanced Research, 9(11), 1018–1030. https://doi.org/10.21474/ijar01/13835
  21. Nefzi, A., Abdallah, R. A. B., Jabnoun-Khiareddine, H., Medimagh-Saidana, S., Haouala, R., & Daami-Remadi, M. (2016). Antifungal activity of aqueous and organic extracts from Withania somnifera L. against Fusarium oxysporum f. sp. radicis-lycopersici. Journal of Microbial & Biochemical Technology, 8(3), 144–150. https://doi.org/10.4172/1948-5948.1000277
  22. Nurlila, R. U., La Fua, J., Abidin, M. S., Sulaeha, S., & Hadju, L. (2022). Effectiveness of endophytic bacteria from local tomato plants against wilt disease caused by Fusarium oxysporum. Caraka Tani: Journal of Sustainable Agriculture, 38(1), 53–64.
  23. Pirayesh, S., Zamanizadeh, H., & Morid, B. (2018). Molecular identification of physiological races of Fusarium oxysporum f. sp. lycopersici and radicis-lycopersici causal agent of Fusarium wilt of tomato in Iran. Journal of Agricultural Science and Technology, 20, 193–202.
  24. R Core Team. (2025). R: A language and environment for statistical computing. R Foundation for Statistical Computing. https://www.R-project.org/
  25. Ramachela, K., Mpaneng, M. A., Mathuthu, M., & Zeevaart, J. R. (2021). Responses of Fusarium oxysporum f. sp. radicis-lycopersici (FORL) to ZnNO₃ under in vitro conditions. Pakistan Journal of Phytopathology, 33(1), 29–35.
  26. Ramírez, V., Martínez, J., Bustillos-Cristales, M. D. R., Castañeda-Antonio, D., Munive, J. A., & Baez, A. (2022). Bacillus cereus MH778713 elicits tomato plant protection against Fusarium oxysporum. Journal of Applied Microbiology, 132(1), 470–482. https://doi.org/10.1111/jam.15179
  27. Saikia, B., Bhattacharyya, A., Boro, R. C., & Savani, A. K. (2025). Bioprospecting of bacterial endophytes from Solanum pimpinellifolium with antifungal activity against Fusarium oxysporum f. sp. lycopersici causing vascular wilt. Physiological and Molecular Plant Pathology, 136, Article 102566. https://doi.org/10.1016/j.pmpp.2025.102566
  28. Sankar, P. M., Raguchander, T., & Shanmugabackiam, S. (2017). Seed bacterization: A novel prime tool for growth promotion in tomato (Solanum lycopersicum M.) under in vitro. International Journal of Current Microbiology and Applied Sciences, 6(11), 3076–3080.
  29. Taiebikhah, N., Mirtalebi, M., & Gharin, R. (2026). Antagonistic potential of arugula vermicompost-derived fungi for controlling tomato crown and root rot caused by Fusarium oxysporum f. sp. radicis-lycopersici. Physiological and Molecular Plant Pathology, 141, Article 102982. https://doi.org/10.1016/j.pmpp.2025.102982
  30. Townsend, G. R., & Heuberger, J. W. (1943). Methods for estimating losses caused by diseases in fungicide experiments. Plant Disease Reporter, 27(17), 340–343.
  31. Tunalı, B., Tosun, C., Maldar, B. M., Meyva, G., & Kansu, B. (2018). Bazı antagonist Trichoderma ve endofitik Acremonium izolatlarının Fusarium kök ve kök-boğazı çürüklüğü hastalıklarına karşı etkinliklerinin belirlenmesi. Türk Tarım-Gıda Bilim ve Teknoloji Dergisi, 6(3), 266–272. https://doi.org/10.24925/turjaf.v6i3.266-272.1613
  32. Validov, S., Kamilova, F., Qi, S., Stephan, D., Wang, J. J., Makarova, N., & Lugtenberg, B. (2007). Selection of bacteria able to control Fusarium oxysporum f. sp. radicis-lycopersici in stonewool substrate. Journal of Applied Microbiology, 102(2), 461–471. https://doi.org/10.1111/j.1365-2672.2006.03083.x
  33. Yang, Y., Wang, Y., Gao, J., Shi, Z., Chen, W., Huangfu, H., Li, Z., & Liu, Y. (2024). Characterisation of Fusarium oxysporum f. sp. radicis-lycopersici in infected tomatoes in Inner Mongolia, China. Journal of Fungi, 10(9), Article 622. https://doi.org/10.3390/jof10090622
  34. Yıldırım, K. C., Canik Orel, D., Okyay, H., Gürsan, M. M., & Demir, İ. (2021). Quality of immature and mature pepper (Capsicum annuum L.) seeds in relation to bio-priming with endophytic Pseudomonas and Bacillus spp. Horticulturae, 7(4), Article 75. https://doi.org/10.3390/horticulturae7040075
  35. Yıldırım, K. C., Canik Orel, D., Okyay, H., Gürsan, M. M., & Yıldız, G. (2025). Enhancing seed quality and plant growth through modification of seed microbiome with beneficial endophytic bacteria. Scientia Horticulturae, 354, Article 114531. https://doi.org/10.1016/j.scienta.2025.114531
  36. Zhang, Z., Li, J., Zhang, Z., Liu, Y., & Wei, Y. (2021). Tomato endophytic bacteria composition and mechanism of suppressiveness of wilt disease (Fusarium oxysporum). Frontiers in Microbiology, 12, Article 731764. https://doi.org/10.3389/fmicb.2021.731764