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Journals / Tekirdağ Ziraat Fakültesi Dergisi / 2026 / Cilt: 23 Sayı: 4

Effect of Different Storage Temperatures and Times on the Pollen Quality of Some Old and Modern Garden Rose Genotypes

Bazı Eski ve Modern Bahçe Gül Genotiplerinin Polen Kalitesi Üzerine Farklı Depolama Sıcaklıkları ve Sürelerinin Etkisi

Fatih Gülbağ(YALOVA ATATÜRK BAHÇE KÜLTÜRLERİ MERKEZ ARAŞTIRMA ENSTİTÜSÜ MÜDÜRLÜĞÜ)0000-0002-6504-4325
Serdar Erken(Atatürk Bahçe Kültürleri Merkez Araştırma Enstitüsü)0000-0003-0682-0942
Yalçın Kaya(Atatürk Bahçe Kültürleri Merkez Araştırma Enstitüsü)0000-0001-8148-7153
Zühtü Polat(Atatürk Bahçe Kültürleri Merkez Araştırma Enstitüsü)0000-0002-4630-6940
Pages
1210–1220
Publication Date
2026-09-28
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These findings demonstrate the importance of evaluating pollen quality and optimizing storage conditions for old garden roses to support their effective use as pollen parents in rose breeding programmes.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Roses (Rosa spp.) are among the most important ornamental plants traded worldwide due to their widespread use as cut flowers, potted plants, landscaping, and medicinal-aromatic purposes.
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Key Findings & Data:Pollen was stored at two different temperatures (+4 °C and -20 °C) for 1 and 12 months.
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Conclusion & Impact:These findings demonstrate the importance of evaluating pollen quality and optimizing storage conditions for old garden roses to support their effective use as pollen parents in rose breeding programmes.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Eski bahçe gülleri · Polen çimlenmesi · Polen depolama

Abstract

Roses (Rosa spp.) are among the most important ornamental plants traded worldwide due to their widespread use as cut flowers, potted plants, landscaping, and medicinal-aromatic purposes. Roses are classified into three main groups: wild roses, old garden roses, and modern roses. Among these groups, old garden roses are extensively used for rose oil production. In rose breeding, achieving adequate seed production is a common challenge. Therefore, identifying parents with high pollen quality is one of the most important factors affecting successful seed set. Furthermore, cost-effective pollen storage methods are required to overcome differences in flowering time between parental genotypes. This study evaluated the viability and germination of fresh and stored pollen from five rose genotypes. Pollen was stored at two different temperatures (+4 °C and -20 °C) for 1 and 12 months. Pollen viability was evaluated using three staining methods: safranin, 2,3,5-triphenyl tetrazolium chloride (TTC), and iodine–potassium iodide (IKI), while germination was assessed using the agar plate method. Viability of fresh pollen varied depending on genotype and staining method, ranging from 12.09% (R. alba, TTC test) to 65.56% (R. hybrida cv. 'Jude the Obscure', safranin test). Germination also differed significantly among genotypes, ranging from 5.07% in R. alba to 49.13% in R. damascena Mill. Storage conditions significantly affected pollen germination; pollen of R. hybrida cv. 'Jude the Obscure' (both storage periods), R. alba 'Semiplena' (1 month), and R. damascena (12 months) exhibited higher germination rates at -20 °C than at +4 °C. These findings demonstrate the importance of evaluating pollen quality and optimizing storage conditions for old garden roses to support their effective use as pollen parents in rose breeding programmes.

Özet

Güller (Rosa spp.), kesme çiçek, saksılı süs bitkisi, peyzaj ve tıbbi-aromatik bitki olarak yaygın şekilde kullanılmaları nedeniyle dünya genelinde ticareti yapılan en önemli süs bitkileri arasında yer almaktadır. Güller üç ana gruba ayrılmaktadır: yabani güller, eski bahçe gülleri ve modern güller. Bu gruplar arasında eski bahçe gülleri özellikle gül yağı üretiminde yaygın olarak kullanılmaktadır. Gül ıslahında yeterli tohum oluşumunun sağlanması yaygın bir zorluktur. Bu nedenle, yüksek polen kalitesine sahip ebeveynlerin belirlenmesi başarılı tohum oluşumunu etkileyen en önemli faktörlerden biridir. Ayrıca, ebeveyn genotipler arasındaki çiçeklenme zamanı farklılıklarını aşabilmek için ekonomik polen depolama yöntemlerine ihtiyaç duyulmaktadır. Bu çalışmada, beş gül genotipine ait taze ve depolanmış polenlerin canlılık ve çimlenme özellikleri değerlendirilmiştir. Polenler iki farklı sıcaklıkta (+4 °C ve −20 °C) 1 ve 12 ay süreyle depolanmıştır. Polen canlılığı: safranin, 2,3,5-trifenil tetrazolyum klorür (TTC) ve iyot–potasyum iyodür (IKI) boyama yöntemleriyle değerlendirilirken, polen çimlenmesi doygun Petri kabı yöntemi kullanılarak belirlenmiştir. Taze polen canlılığı, genotipe ve kullanılan boyama yöntemine bağlı olarak %12,09 (R. alba, TTC testi) ile %65,56 (R. hybrida cv. 'Jude the Obscure', safranin testi) arasında değişmiştir. Polen çimlenmesi de genotipler arasında önemli farklılık göstermiş ve R. alba’da %5,07 ile R. damascena Mill.’de %49,13 arasında bulunmuştur. Depolama koşulları polen çimlenmesini önemli ölçüde etkilemiştir; R. hybrida cv. 'Jude the Obscure' her iki depolama süresinde, R. alba 'Semiplena' 1 aylık depolama sonrasında ve R. damascena 12 aylık depolama sonrasında, +4 °C'ye kıyasla −20 °C'de daha yüksek çimlenme oranları göstermiştir. Bu bulgular, eski bahçe güllerinin gül ıslahında polen ebeveyni olarak etkin kullanımını desteklemek amacıyla polen kalitesinin değerlendirilmesi ve uygun depolama koşullarının belirlenmesinin önemini ortaya koymaktadır.

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Cite This Article

Gülbağ, F., Erken, S., Kaya, Y., Polat, Z. (2026). Effect of Different Storage Temperatures and Times on the Pollen Quality of Some Old and Modern Garden Rose Genotypes. Tekirdağ Ziraat Fakültesi Dergisi, Cilt: 23 Sayı: 4, 1210–1220. https://doi.org/10.33462/jotaf.1662571

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References (38)

  1. Almeida, N. V. D., Saziki, C. Y. N. and Cardoso, J. C. (2019). Characterization of cultivars and low-temperature pollen grain storage in amaryllis (Hippeastrum sp.). Revista Ceres, 66: 451-459.
  2. Bıtrak, O. O. and Hatırlı, S. A. (2025). Analysis of production efficiency in oil-bearing rose cultivation: The case of Isparta Province. Journal of Tekirdag Agricultural Faculty, 22(1): 1-17. https://doi.org/10.33462/jotaf.1259314 (In Turkish)
  3. Borghezan, M., Clauman, A. D., Steinmacher, D. A., Guerra, M. P. and Orth, A. I. (2011). In vitro viability and preservation of pollen grain of kiwi (Actinidia chinensis var. deliciosa (A. Chev.) A. Chev)., Crop Breeding and Applied Biotechnology, 11: 338-344.
  4. Cairns, T. (2003). Horticultural Classification Schemes. In: Encyclopedia of Rose Science, Vol. 1., Ed(s): Roberts A. V., Debener T. and Gudin, S., Elsevier, Amsterdam, Netherlands. https://doi.org/10.1016/B0-12-227620-5/00022-7
  5. Caser M. (2017). Pollen Grains and Tubes. In: Reference Module in Life Sciences, Ed(s): Pentimalli, F., Giordano, A., O'Neill, S., Ranganathan, S., Sharfstein, S., Yelon, D., Dixon, B., Cotter, P. D., Hernandez, O. Z., Zhou, H. X., Roitberg, B., Vitale, I., Elsevier. https://doi.org/10.1016/B978-0-12-809633-8.05077-9
  6. Damayanti, F., Garvita, R. V., Wawangningrum, H. and Rahayu, S. (2021). Flower development, pollen viability and pollen storage test of Aeschynanthus radicans. Biodiversitas Journal of Biological Diversity, 22(4): 1940-1945. https://doi.org/10.13057/biodiv/d220442
  7. Datta, S. K. (2018). Breeding of new ornamental varieties. Current Science, 114(6): 1194-1206.
  8. Du, G., Xu, J., Gao, C., Lu, J., Li, Q., Du, J., Lv M. and Sun, X. (2019). Effect of low storage temperature on pollen viability of fifteen herbaceous peonies. Biotechnology Reports, 21: e00309. https://doi.org/10.1016/j.btre.2019.e00309
  9. Erbaş, S., Alagöz, M. and Baydar, H. (2015). A Research on Flower Morphology and Pollen Viabilty of Oil-bearing Rose (Rosa damascena Mill.). Journal of Agriculture Faculty, 10(2): 40-50. https://doi.org/10.19113/sdufenbed.1021987 (In Turkish)
  10. Ercişli, S. (2007). Determination of pollen viability and in vitro pollen germination of Rosa dumalis and Rosa villosa. Bangladesh Journal of Botany, 36(2): 185-187. https://doi.org/10.3329/bjb.v36i2.1511
  11. Erken, S., Gülbağ, F., Erken, K., Kazaz, S. and Özzambak, M. E. (2018). Development of new rose varieties through hybridization. General Directorate of Agricultural Research, Project Report, Project No: TAGEM/BBAD/14/A09/P08/01-II. Publication No:359. Yalova. (In Turkish)
  12. Eti, S. (1990). A practical method for determining pollen quantity. Journal of the Faculty of Agriculture, Çukurova University, 5(4): 49–58. (In Turkish)
  13. Eti, S. (1991). Determination of pollen viability and germination ability in some fruit species and cultivars using different in vitro tests. Journal of the Faculty of Agriculture, Çukurova University, 6(1): 69–80. (In Turkish)
  14. Farooq, A., Kiani, M., Khan, M. A., Riaz, A., Khan, A. A., Anderson, N. and Byrne, D. H. (2013). Microsatellite analysis of Rosa damascena from Pakistan and Iran. Horticulture, Environment, and Biotechnology, 54: 141-147. https://doi.org/10.1007/s13580-013-0042-x
  15. Fragallah, S. A. D. A., Lin, S., Li, N., Ligate, E. J. and Chen, Y. (2019). Effects of sucrose, boric acid, pH, and incubation time on in vitro germination of pollen and tube growth of Chinese fir (Cunnighamial lanceolata L.). Forests, 10(2): 102. https://doi.org/10.3390/f10020102
  16. Giovannini, A., Macovei, A., Donà, M., Valassi, A., Caser, M., Mansuino, A., Ghione, G. G., Carbonera, D., Scariot, V. and Balestrazzi, A. (2015). Pollen grain preservation at low temperatures in valuable commercial rose cultivars. Acta Horticulturae, 1064: 63-66.
  17. Giovannini, A., Macovei, A., Caser, M., Mansuino, A., Ghione, G. G., Savona, M., Carbonera, D., Scariot, V. and Balestrazzi, A. (2017). Pollen grain preservation and fertility in valuable commercial rose cultivars. Plants, 6(2): 17. https://doi.org/10.3390/plants6020017
  18. Khan, S. A. and Perveen, A. (2014). In vitro pollen germination of five citrus species. Pakistan Journal of Botany, 46(3): 951-956.
  19. Khan, M. F., Hafiz, I. A., Khan, M. A., Abbasi, N. A., Habib, U. and Shah, M. K. N. (2021). Determination of pollen fertility and hybridization success among Rosa hybrida. Pakistan Journal of Botany, 53(5): 1791-1800. https://doi.org/10.30848/PJB2021-5(15)
  20. Khosh-Khui, M., Bassiri, A. and Niknejad, M. (1976). Effects of temperature and humidity on pollen viability of six rose species. Canadian Journal of Plant Science. 56 (3): 517-523. https://doi.org/10.4141/cjps76-085
  21. Kılıç, T. (2020). Breeding of fragrant cut roses through hybridization. (Ph.D. Thesis) Ankara University, Graduate School of Natural and Applied Sciences, Ankara, Türkiye. (In Turkish)
  22. Korkut, S. S., Kazaz. S. and Kılıç, T. (2022). Different storage temperatures and times on pollen quality in cut rose varieties. Ornamental Horticulture 28: 202-211. https://doi.org/10.1590/2447-536x.v28i2.2470
  23. Leus, L., Van Laere, K., De Riek, J. and Van Huylenbroeck, J. (2018). Rose. In: Handbook of Plant Breeding, Ornamental Crops, Vol 11., Ed(s): Van Huylenbroeck, J., Springer, Cham. https://doi.org/10.1007/978-3-319-90698-0_27
  24. Martins, E. S., Davide, L. M. C., Miranda, G. J., Barizon, J. O., Souza Junior, F., Carvalho, R. P. and Gonçalves, M. C. (2017). In vitro pollen viability of maize cultivars at different times of collection. Ciencia Rural, 47(02):1-8. https://doi.org/10.1590/0103-8478cr20151077
  25. Mondo, J. M., Agre, P. A., Asiedu, R., Akoroda, M. O. and Asfaw, A. (2021). Optimized protocol for in vitro pollen germination in yam (Dioscorea spp.). Plants, 10(4): 795. https://doi.org/10.3390/plants10040795
  26. Nadeem, M., Akond, M., Riaz, A., Qasim, M., Younis, A. and Farooq, A. (2013). Pollen morphology and viability relates to seed production in hybrid roses. Plant Breeding and Seed Science, 68(1): 25-38. https://doi.org/10.2478/v10129-011-0078-y
  27. Parton, E., Vervaeke, I., Delen, R., Vandenbussche, B., Deroose, R. and De Proft, M. (2002). Viability and storage of bromeliad pollen. Euphytica, 125:155-161.
  28. Paupière, M. J., Müller, F., Li, H., Rieu, I., Tikunov, Y. M., Visser, R. G. and Bovy, A. G. (2017). Untargeted metabolomic analysis of tomato pollen development and heat stress response. Plant Reproduction, 30(2): 81-94. https://doi.org/10.1007/s00497-017-0301-6
  29. Pipino, L., Van Labeke, M. C., Mansuino, A., Scariot, V., Giovannini, A. and Leus, L. (2011). Pollen morphology as fertility predictor in hybrid tea roses. Euphytica, 178: 203-214. https://doi.org/10.1007/s10681-010-0298-5
  30. Schulz, H. (2003). Fragrance and Pigments/Odoriferous Substances and Pigments. In: Encyclopedia of Rose Science, Ed(s): Roberts, A. V., Debener, T. and Gudin, S., Elsevier, Amsterdam, Netherlands. https://doi.org/10.1016/B0-12-227620-5/00070-7
  31. Seyhan, S. (2020). Effects of different storage temperatures and durations on pollen quality and germination capacity of some rose species distributed in the flora of Türkiye. (MSc. Thesis) Ankara University, Graduate School of Natural and Applied Sciences, Ankara, Türkiye. (In Turkish)
  32. Shivanna, K. R. (2003). Pollen Biology and Biotechnology; Science Publishers Inc.: Enfield, NH, U. S. A. Tuinstra, M. R. and Wedel, J. (2000). Estimation of pollen viability in grain sorghum. Crop Science, 40(4): 968-970. https://doi.org/10.2135/cropsci2000.404968x
  33. Vargas, D. P., Souza, S. A. M., Anjos e Silva, S. D. and Bobrowski, V. L. (2009). Analysis of pollen grains from different castor bean (Ricinus communis L., Euphorbiaceae) cultivars: conservation and viability. Arquivos do Instituto Biológico, 76(1): 115-120. https://doi.org/10.1590/1808-1657v76p1152009 (In Portuguese)
  34. Wang, L., Wu, J., Chen, J., Fu, D., Zhang, C., Cai, C. and Ou, L. (2015). A simple pollen collection, dehydration, and long-term storage method for litchi (Litchi chinensis Sonn.). Scientia Horticulturae, 188: 78-83. https://doi.org/10.1016/j.scienta.2015.03.021
  35. Yücel, S. A. and Kıvan, M. (2018). Pest Hemiptera and Hymenoptera species found in the rose garden of Göztepe Park, Istanbul. Journal of Tekirdag Agricultural Faculty, 15(2): 95–100. (In Turkish)
  36. Zambon, C. R., Silva, L. F. D. O. D., Pio, R., Bianchini, F. G. and Oliveira, A. F. D. (2018). Storage of pollen and properties of olive stigma for breeding purposes. Revista Ciência Agronômica, 49: 291-297. https://doi.org/10.5935/1806-6690.20180033
  37. Zlesak, D. C. (2007). Rose, Rosa x hybrida. In: Flower Breeding and Genetics, Ed(s): Anderson, N. O., Springer, Dordrecht, Netherlands. https://doi.org/10.1007/978-1-4020-4428-1_26
  38. Zlesak, D. C., Zuzek, K. and Hokanson, S. C. (2007). Rose pollen viability over time at varying storage temperatures. Acta Horticulturae, (751): 337–341. https://doi.org/10.17660/ActaHortic.2007.751.43