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Journals / Ordu Üniversitesi Bilim ve Teknoloji Dergisi / 2025 / Cilt: 15 Sayı: 2

In Vitro Responses of Different Radish (Raphanus sativus L.) Varieties to Drought Stress Induced by Polyethylene Glycol (PEG)

Polietilen Glikol (PEG) ile Oluşturulan Kuraklık Stresine Karşı Farklı Turp (Raphanus sativus L.) Çeşitlerinin İn Vitro Tepkileri

Ecem Kara(SİVAS BİLİM VE TEKNOLOJİ ÜNİVERSİTESİ)0000-0002-0118-2673
Pages
372–386
Publication Date
2025-12-27
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The results indicated that increasing PEG concentration had a statistically significant negative effect on all evaluated parameters.

📌 3 Key Takeaways

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Objective & Hypothesis:This study was carried out to determine the morphological responses of two different radish varieties (Raphanus sativus L.) to artificial drought stress induced by using different levels of polyethylene glycol (PEG) under in vitro conditions.Kırmızı inci (TP1) and Chery belle (TP2) radish varietirs were used, and four PEG concentrations (0%, 2%, 4%, and 6%) were added to the Murashige and Skoog (MS) basal medium.
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Key Findings & Data:The results indicated that increasing PEG concentration had a statistically significant negative effect on all evaluated parameters.
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Conclusion & Impact:The findings suggest that higher PEG concentrations limit water uptake and cell elongation, leading to reduced metabolic activity in plants, while certain genotypes exhibit a greater tolerance capacity to water deficit conditions.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Raphanus sativus L. · polietilen glikol (PEG) · in vitro

Abstract

This study was carried out to determine the morphological responses of two different radish varieties (Raphanus sativus L.) to artificial drought stress induced by using different levels of polyethylene glycol (PEG) under in vitro conditions.Kırmızı inci (TP1) and Chery belle (TP2) radish varietirs were used, and four PEG concentrations (0%, 2%, 4%, and 6%) were added to the Murashige and Skoog (MS) basal medium. The results indicated that increasing PEG concentration had a statistically significant negative effect on all evaluated parameters. As the PEG level increased, germination rate, root and shoot length, fresh and dry weights decreased. However, significant varietal differences were observed and the Kırmızı inci (TP1) variety exhibited higher values, particularly in shoot and root fresh/dry weight and germination rate, compared to Chery belle (TP2). The findings suggest that higher PEG concentrations limit water uptake and cell elongation, leading to reduced metabolic activity in plants, while certain genotypes exhibit a greater tolerance capacity to water deficit conditions.

Özet

Bu çalışma, iki farklı turp çeşidinin (Raphanus sativus L.) in vitro koşullarda farklı düzeylerde polietilen glikol (PEG) kullanılarak oluşturulan yapay kuraklık stresine verdikleri morfolojik tepkileri belirlemek amacıyla gerçekleştirilmiştir. Araştırmada, Kırmızı inci (TP1) ve Chery belle (TP2) turp çeşitleri kullanılmış olup, PEG’in dört farklı konsantrasyonu (%0, %2, %4 ve %6) Murashige ve Skoog (MS) besin ortamına ilave edilmiştir. Elde edilen sonuçlar, PEG düzeyindeki artışın tüm incelenen parametreler üzerinde istatistiksel olarak anlamlı düzeyde olumsuz etki oluşturduğunu göstermiştir. PEG konsantrasyonu yükseldikçe çimlenme oranı, kök ve gövde uzunluğu ile yaş ve kuru ağırlık değerleri azalmıştır. Bununla birlikte, çeşitler arasında belirgin farklılıklar gözlemlenmiş; Kırmızı inci (TP1) çeşidi, özellikle gövde ve kök yaş/kuru ağırlığı ile çimlenme oranı bakımından Chery belle (TP2) çeşidine kıyasla daha yüksek değerlere ulaşmıştır. Sonuçlar, artan PEG konsantrasyonlarının bitkilerde su alımını ve hücre uzamasını sınırlayarak metabolik faaliyetleri yavaşlattığını, ancak bazı genotiplerin bu strese karşı daha yüksek tolerans kapasitesine sahip olduğunu ortaya koymuştur.

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Cite This Article

Kara, E. (2025). In Vitro Responses of Different Radish (Raphanus sativus L.) Varieties to Drought Stress Induced by Polyethylene Glycol (PEG). Ordu Üniversitesi Bilim ve Teknoloji Dergisi, Cilt: 15 Sayı: 2, 372–386. https://doi.org/10.54370/ordubtd.1804349

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