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Journals / EurAsian Journal of Oral and Maxillofacial Surgery / 2026 / Volume: 4 Issue: 2

Comparison of Dental Follicle Stem Cells and Dental Pulp Stem Cells in a Translational Bone Tissue Engineering Protocol

Translasyonel Kemik Doku Mühendisliği Protokolünde Dental Folikül Kök Hücreleri ile Dental Pulpa Kök Hücrelerinin Karşılaştırılması

Elif Ozcan0000-0002-4435-5124
Yücel Erbilgin(ISTANBUL UNIVERSITY, AZIZ SANCAR INSTITUTE OF EXPERIMENTAL MEDICINE)0009-0005-3691-188X
Selin Yıldırım0009-0003-3096-2825
Noushin Zibandeh(University of Oxford)0000-0002-4078-8029
Tunç Akkoç(Marmara University)0000-0001-9179-2805
Kamil Goker0009-0005-4600-0721
Pages
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Publication Date
2025-05-29
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This complex is a promising tool for maxillofacial tissue engineering applications.

📌 3 Key Takeaways

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Objective & Hypothesis:Purpose: In this study, it is aimed to establish and refine a translational protocol and compare the osteogenic potential of DPSC and DFSC’s on nano mesh containing (nmPCL) and plain (PCL) polycaprolactone scaffolds in vitro and contribute the translational medicine protocols in bone regeneration.
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Key Findings & Data:Materials and Methods: DPSCs and DFSCs were osteogenically differentiated on PCL and nmPCL scaffolds and four groups were examined for cell proliferation and type I collagen formation rates after two weeks of culture.
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Conclusion & Impact:This complex is a promising tool for maxillofacial tissue engineering applications.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Dental Follicle Stem Cell · Dental Pulp Stem Cell · Polycaprolactone

Abstract

Purpose: In this study, it is aimed to establish and refine a translational protocol and compare the osteogenic potential of DPSC and DFSC’s on nano mesh containing (nmPCL) and plain (PCL) polycaprolactone scaffolds in vitro and contribute the translational medicine protocols in bone regeneration. Materials and Methods: DPSCs and DFSCs were osteogenically differentiated on PCL and nmPCL scaffolds and four groups were examined for cell proliferation and type I collagen formation rates after two weeks of culture. Following immunofluorescence labeling, Nonparametric (Kruskal Wallis) and multiple comparison tests were used to compare the four groups. Results: Among all groups, mean cell counts on scaffolds ranged from 30.8 to 82.6 cells/0.0915 mm2, and total collagen formation ranged from 2.79% to 17,9%. DFSC and nmPCL complex showed significantly higher cell counts (p< 0,01) and collagen formation rates (p< 0,01) in comparison to other groups. Conclusion: DFSC/nmPCL group is found to show superior properties on cell proliferation and bone matrix formation. This complex is a promising tool for maxillofacial tissue engineering applications.

Özet

Amaç: Bu çalışmada, DPSC ve DFSC'lerin nanomesh içeren (nmPCL) ve düz (PCL) polikaprolakton iskeleler üzerindeki osteojenik potansiyellerini in vitro olarak karşılaştırmak ve kemik rejenerasyonunda translasyonel tıp protokollerine katkıda bulunmak amaçlanmıştır. Gereç ve Yöntemler: DPSC'ler ve DFSC'ler PCL ve nmPCL iskeleleri üzerinde osteojenik olarak farklılaştırılmış ve iki haftalık kültür sonrasında dört grup hücre proliferasyonu ve tip I kollajen oluşum oranları açısından incelenmiştir. İmmünofloresan etiketlemenin ardından, dört grubu karşılaştırmak için parametrik olmayan (Kruskal Wallis) ve çoklu karşılaştırma testleri kullanılmıştır. Sonuçlar: Tüm gruplar arasında, iskelelerdeki ortalama hücre sayıları 30.8 ila 82.6 hücre/0.0915 mm2 arasında ve toplam kolajen oluşumu %2.79 ila %17,9 arasında değişmektedir. DFSC ve nmPCL kompleksi diğer gruplara kıyasla anlamlı olarak daha yüksek hücre sayısı (p< 0,01) ve kolajen oluşum oranları (p< 0,01) göstermiştir. Sonuç: DFSC/nmPCL grubunun hücre proliferasyonu ve kemik matriksi oluşumu üzerinde üstün özellikler gösterdiği bulunmuştur. Bu kompleks maksillofasiyal doku mühendisliği uygulamaları için umut verici bir araçtır.

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Cite This Article

Ozcan, E., Erbilgin, Y., Yıldırım, S., Zibandeh, N., Akkoç, T., Goker, K. (2025). Comparison of Dental Follicle Stem Cells and Dental Pulp Stem Cells in a Translational Bone Tissue Engineering Protocol. EurAsian Journal of Oral and Maxillofacial Surgery, Volume: 4 Issue: 2. https://ajindex.com/articles/1629627

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